Magnesium in PDB 6ow3: X-Ray Crystal Structure of A Bacterial Reiterative Transcription Complex of Pyrg Promoter Variant -1T

All present enzymatic activity of X-Ray Crystal Structure of A Bacterial Reiterative Transcription Complex of Pyrg Promoter Variant -1T:
2.7.7.6;

The structure of X-Ray Crystal Structure of A Bacterial Reiterative Transcription Complex of Pyrg Promoter Variant -1T, PDB code: 6ow3 was solved by Y.Shin, K.S.Murakami, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.87 / 2.77
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	186.187, 101.833, 295.755, 90.00, 98.64, 90.00
R / Rfree (%)	21.7 / 25.5

The structure of X-Ray Crystal Structure of A Bacterial Reiterative Transcription Complex of Pyrg Promoter Variant -1T also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Magnesium atom in the X-Ray Crystal Structure of A Bacterial Reiterative Transcription Complex of Pyrg Promoter Variant -1T (pdb code 6ow3). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the X-Ray Crystal Structure of A Bacterial Reiterative Transcription Complex of Pyrg Promoter Variant -1T, PDB code: 6ow3:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the X-Ray Crystal Structure of A Bacterial Reiterative Transcription Complex of Pyrg Promoter Variant -1T


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of X-Ray Crystal Structure of A Bacterial Reiterative Transcription Complex of Pyrg Promoter Variant -1T within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg1601 b:71.1 occ:1.00 O D:LYS840 2.0 75.8 1.0 OE2 B:GLU154 2.6 89.0 1.0 C D:LYS840 3.1 72.4 1.0 CD B:GLU154 3.7 91.9 1.0 CA D:LYS840 3.8 70.2 1.0 O D:LEU839 3.9 74.5 1.0 OD2 B:ASP168 4.0 69.9 1.0 OE1 B:GLU154 4.1 91.6 1.0 OE1 B:GLU64 4.2 0.8 1.0 N D:TYR841 4.3 74.8 1.0 OD1 B:ASP168 4.3 74.0 1.0 CG B:ASP168 4.6 74.5 1.0 OE2 B:GLU64 4.7 97.7 1.0 N D:LYS840 4.7 70.2 1.0 C D:LEU839 4.7 74.0 1.0 CA D:TYR841 4.7 67.5 1.0 CG D:LYS840 4.8 61.3 1.0 CB D:LYS840 4.8 61.1 1.0 CD B:GLU64 4.9 0.5 1.0 CG B:GLU154 4.9 90.6 1.0 N D:VAL842 5.0 64.4 1.0

Magnesium binding site 2 out of 2 in the X-Ray Crystal Structure of A Bacterial Reiterative Transcription Complex of Pyrg Promoter Variant -1T


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of X-Ray Crystal Structure of A Bacterial Reiterative Transcription Complex of Pyrg Promoter Variant -1T within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg1603 b:50.4 occ:1.00 OD1 D:ASP739 1.9 65.2 1.0 O3' I:G5 2.0 61.3 1.0 OD1 D:ASP743 2.2 51.0 1.0 OD1 D:ASP741 2.3 54.4 1.0 OD2 D:ASP741 2.7 71.2 1.0 CG D:ASP741 2.9 61.5 1.0 CG D:ASP743 3.0 52.3 1.0 OD2 D:ASP743 3.0 55.8 1.0 CG D:ASP739 3.1 61.0 1.0 C3' I:G5 3.3 52.9 1.0 O2' I:G5 3.6 55.6 1.0 CB D:ASP739 3.7 60.8 1.0 C4' I:G5 3.8 55.0 1.0 C2' I:G5 4.0 54.5 1.0 OD2 D:ASP739 4.1 57.7 1.0 N D:ASP739 4.2 55.1 1.0 CA D:ASP739 4.3 51.7 1.0 CB D:ASP741 4.4 55.2 1.0 C5' I:G5 4.5 51.1 1.0 NH2 D:ARG704 4.5 53.8 1.0 CB D:ASP743 4.5 43.7 1.0 C D:ASP739 4.7 58.9 1.0 N D:ASP741 4.7 54.1 1.0 N D:ASP743 4.8 49.9 1.0 O D:ASP739 4.9 55.7 1.0 CA D:ASP741 5.0 51.4 1.0

K.S.Murakami, Y.Shin. Structural Basis of Reiterative Transcription From the Pyrg Promoter To Be Published.