Magnesium in PDB 6p07: Spastin Hexamer in Complex with Substrate

All present enzymatic activity of Spastin Hexamer in Complex with Substrate:
5.6.1.1;

The binding sites of Magnesium atom in the Spastin Hexamer in Complex with Substrate (pdb code 6p07). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 6 binding sites of Magnesium where determined in the Spastin Hexamer in Complex with Substrate, PDB code: 6p07:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6;

Magnesium binding site 1 out of 6 in the Spastin Hexamer in Complex with Substrate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Spastin Hexamer in Complex with Substrate within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg802 b:76.4 occ:1.00 O2B A:ATP801 2.0 90.6 1.0 OG1 A:THR530 2.5 91.6 1.0 O1A A:ATP801 3.1 90.6 1.0 O3G A:ATP801 3.1 90.6 1.0 PB A:ATP801 3.3 90.6 1.0 NH2 B:ARG641 3.6 79.9 1.0 CB A:THR530 3.7 91.6 1.0 O3B A:ATP801 3.8 90.6 1.0 O3A A:ATP801 3.8 90.6 1.0 PA A:ATP801 4.1 90.6 1.0 PG A:ATP801 4.2 90.6 1.0 NH2 B:ARG640 4.4 81.2 1.0 CG2 A:THR530 4.4 91.6 1.0 OE1 A:GLN583 4.6 94.2 1.0 O1B A:ATP801 4.6 90.6 1.0 N A:THR530 4.7 91.6 1.0 OD2 B:ASP611 4.7 88.1 1.0 CZ B:ARG641 4.8 79.9 1.0 CA A:THR530 4.8 91.6 1.0 O1G A:ATP801 4.9 90.6 1.0 O2A A:ATP801 4.9 90.6 1.0

Magnesium binding site 2 out of 6 in the Spastin Hexamer in Complex with Substrate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Spastin Hexamer in Complex with Substrate within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg802 b:65.5 occ:1.00 O2B B:ATP801 2.0 70.3 1.0 O2G B:ATP801 2.2 70.3 1.0 OG1 B:THR530 2.4 71.5 1.0 OD2 B:ASP582 3.0 72.7 1.0 PB B:ATP801 3.3 70.3 1.0 OD1 B:ASP582 3.4 72.7 1.0 CB B:THR530 3.5 71.5 1.0 PG B:ATP801 3.5 70.3 1.0 O3B B:ATP801 3.6 70.3 1.0 CG B:ASP582 3.6 72.7 1.0 NE2 B:GLN583 4.1 69.3 1.0 CG2 B:THR530 4.1 71.5 1.0 NH2 C:ARG641 4.3 67.2 1.0 O3A B:ATP801 4.4 70.3 1.0 O1B B:ATP801 4.4 70.3 1.0 O1G B:ATP801 4.4 70.3 1.0 O3G B:ATP801 4.6 70.3 1.0 O1A B:ATP801 4.6 70.3 1.0 OD2 C:ASP611 4.6 76.1 1.0 CA B:THR530 4.7 71.5 1.0 N B:THR530 4.8 71.5 1.0

Magnesium binding site 3 out of 6 in the Spastin Hexamer in Complex with Substrate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Spastin Hexamer in Complex with Substrate within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg802 b:50.5 occ:1.00 O2B C:ATP801 2.0 61.0 1.0 OG1 C:THR530 2.3 61.1 1.0 O1G C:ATP801 2.3 61.0 1.0 PB C:ATP801 3.3 61.0 1.0 PG C:ATP801 3.4 61.0 1.0 OD2 C:ASP582 3.5 61.9 1.0 O3G C:ATP801 3.5 61.0 1.0 CB C:THR530 3.5 61.1 1.0 OD1 C:ASP582 3.6 61.9 1.0 O3B C:ATP801 3.8 61.0 1.0 NE2 C:GLN583 3.9 60.6 1.0 CG C:ASP582 4.0 61.9 1.0 O1B C:ATP801 4.1 61.0 1.0 O1A C:ATP801 4.2 61.0 1.0 CG2 C:THR530 4.4 61.1 1.0 N C:THR530 4.5 61.1 1.0 NH2 D:ARG641 4.5 62.8 1.0 O3A C:ATP801 4.5 61.0 1.0 CA C:THR530 4.6 61.1 1.0 O2G C:ATP801 4.7 61.0 1.0 NH2 D:ARG640 4.9 62.2 1.0 PA C:ATP801 4.9 61.0 1.0

Magnesium binding site 4 out of 6 in the Spastin Hexamer in Complex with Substrate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Spastin Hexamer in Complex with Substrate within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg802 b:63.2 occ:1.00 O3G D:ATP801 2.0 62.7 1.0 O1B D:ATP801 2.1 62.7 1.0 OG1 D:THR530 2.2 63.4 1.0 OD2 D:ASP582 3.1 66.3 1.0 CB D:THR530 3.3 63.4 1.0 PB D:ATP801 3.3 62.7 1.0 PG D:ATP801 3.3 62.7 1.0 O3B D:ATP801 3.5 62.7 1.0 O1A D:ATP801 3.5 62.7 1.0 OD2 E:ASP611 3.5 71.7 1.0 OD1 D:ASP582 3.6 66.3 1.0 NH2 E:ARG641 3.6 65.5 1.0 CG D:ASP582 3.7 66.3 1.0 CG2 D:THR530 4.1 63.4 1.0 O1G D:ATP801 4.1 62.7 1.0 O3A D:ATP801 4.2 62.7 1.0 N D:THR530 4.4 63.4 1.0 O2G D:ATP801 4.4 62.7 1.0 PA D:ATP801 4.5 62.7 1.0 CA D:THR530 4.5 63.4 1.0 NE2 D:GLN583 4.5 63.5 1.0 O2B D:ATP801 4.5 62.7 1.0 CG E:ASP611 4.6 71.7 1.0 CZ E:ARG641 4.8 65.5 1.0

Magnesium binding site 5 out of 6 in the Spastin Hexamer in Complex with Substrate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Spastin Hexamer in Complex with Substrate within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg802 b:77.2 occ:1.00 O2B E:ATP801 2.1 92.0 1.0 OG1 E:THR530 2.2 83.5 1.0 OD2 E:ASP582 2.9 80.4 1.0 OD2 F:ASP611 3.1 1.0 1.0 PB E:ATP801 3.3 92.0 1.0 OD1 E:ASP582 3.3 80.4 1.0 CB E:THR530 3.5 83.5 1.0 O1A E:ATP801 3.5 92.0 1.0 O3A E:ATP801 3.5 92.0 1.0 CG E:ASP582 3.5 80.4 1.0 O3B E:ATP801 3.8 92.0 1.0 NH2 F:ARG641 3.9 0.8 1.0 CG2 E:THR530 4.1 83.5 1.0 PA E:ATP801 4.2 92.0 1.0 CG F:ASP611 4.2 1.0 1.0 NE2 E:GLN583 4.3 82.7 1.0 CA E:THR530 4.5 83.5 1.0 N E:THR530 4.6 83.5 1.0 O1B E:ATP801 4.6 92.0 1.0 O3G E:ATP801 4.7 92.0 1.0 PG E:ATP801 4.9 92.0 1.0 OD1 F:ASP611 4.9 1.0 1.0

Magnesium binding site 6 out of 6 in the Spastin Hexamer in Complex with Substrate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of Spastin Hexamer in Complex with Substrate within 5.0Å range: probe atom residue distance (Å) B Occ F:Mg803 b:0.1 occ:1.00 O3B F:ADP802 2.0 0.2 0.5 O1B F:ATP801 2.1 0.0 0.5 OG1 F:THR530 2.2 0.1 1.0 O2A F:ADP802 2.9 0.2 0.5 O3B F:ATP801 3.1 0.0 0.5 PB F:ATP801 3.1 0.0 0.5 PB F:ADP802 3.4 0.2 0.5 CB F:THR530 3.5 0.1 1.0 PA F:ADP802 3.6 0.2 0.5 O1A F:ADP802 3.9 0.2 0.5 O2B F:ADP802 3.9 0.2 0.5 O3G F:ATP801 3.9 0.0 0.5 O3A F:ADP802 4.0 0.2 0.5 O3A F:ATP801 4.1 0.0 0.5 PG F:ATP801 4.1 0.0 0.5 O1A F:ATP801 4.1 0.0 0.5 CG2 F:THR530 4.2 0.1 1.0 O2B F:ATP801 4.3 0.0 0.5 O2G F:ATP801 4.5 0.0 0.5 CA F:THR530 4.6 0.1 1.0 O1B F:ADP802 4.6 0.2 0.5 N F:THR530 4.6 0.1 1.0 PA F:ATP801 4.6 0.0 0.5 NE2 F:GLN583 4.6 0.5 1.0 OD1 F:ASP582 4.6 0.9 1.0 CB F:LYS529 4.9 0.0 1.0 C F:LYS529 4.9 0.0 1.0 CG F:LYS529 5.0 0.0 1.0 CD F:LYS529 5.0 0.0 1.0

C.R.Sandate, A.Szyk, E.A.Zehr, G.C.Lander, A.Roll-Mecak. An Allosteric Network in Spastin Couples Multiple Activities Required For Microtubule Severing. Nat.Struct.Mol.Biol. V. 26 671 2019.
ISSN: ESSN 1545-9985
PubMed: 31285604
DOI: 10.1038/S41594-019-0257-3