Magnesium in PDB 6q21: Molecular Switch For Signal Transduction: Structural Differences Between Active and Inactive Forms of Protooncogenic Ras Proteins
Protein crystallography data
The structure of Molecular Switch For Signal Transduction: Structural Differences Between Active and Inactive Forms of Protooncogenic Ras Proteins, PDB code: 6q21
was solved by
S.-H.Kim,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
N/A /
1.95
|
Space group
|
P 1 1 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
41.980,
79.120,
128.500,
90.00,
90.00,
117.48
|
R / Rfree (%)
|
19 /
n/a
|
Magnesium Binding Sites:
The binding sites of Magnesium atom in the Molecular Switch For Signal Transduction: Structural Differences Between Active and Inactive Forms of Protooncogenic Ras Proteins
(pdb code 6q21). This binding sites where shown within
5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the
Molecular Switch For Signal Transduction: Structural Differences Between Active and Inactive Forms of Protooncogenic Ras Proteins, PDB code: 6q21:
Jump to Magnesium binding site number:
1;
2;
3;
4;
Magnesium binding site 1 out
of 4 in 6q21
Go back to
Magnesium Binding Sites List in 6q21
Magnesium binding site 1 out
of 4 in the Molecular Switch For Signal Transduction: Structural Differences Between Active and Inactive Forms of Protooncogenic Ras Proteins
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 1 of Molecular Switch For Signal Transduction: Structural Differences Between Active and Inactive Forms of Protooncogenic Ras Proteins within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mg173
b:34.1
occ:1.00
|
O
|
A:HOH801
|
1.9
|
26.8
|
1.0
|
O1G
|
A:GCP180
|
2.0
|
45.4
|
1.0
|
O
|
A:HOH800
|
2.1
|
35.5
|
1.0
|
O1B
|
A:GCP180
|
2.1
|
42.1
|
1.0
|
OG1
|
A:THR35
|
2.3
|
66.5
|
1.0
|
OG
|
A:SER17
|
2.4
|
28.7
|
1.0
|
PG
|
A:GCP180
|
3.1
|
56.1
|
1.0
|
C3B
|
A:GCP180
|
3.2
|
50.0
|
1.0
|
CB
|
A:THR35
|
3.2
|
65.6
|
1.0
|
PB
|
A:GCP180
|
3.2
|
42.6
|
1.0
|
CB
|
A:SER17
|
3.4
|
36.7
|
1.0
|
O2G
|
A:GCP180
|
3.8
|
51.0
|
1.0
|
O
|
A:THR58
|
3.9
|
35.1
|
1.0
|
N
|
A:SER17
|
4.0
|
41.5
|
1.0
|
OD2
|
A:ASP57
|
4.0
|
36.1
|
1.0
|
OD1
|
A:ASP57
|
4.0
|
50.8
|
1.0
|
CG2
|
A:THR35
|
4.1
|
68.5
|
1.0
|
NZ
|
A:LYS16
|
4.1
|
26.9
|
1.0
|
O2B
|
A:GCP180
|
4.2
|
32.5
|
1.0
|
N
|
A:THR35
|
4.2
|
59.9
|
1.0
|
CA
|
A:SER17
|
4.2
|
35.1
|
1.0
|
O3G
|
A:GCP180
|
4.3
|
50.1
|
1.0
|
O3A
|
A:GCP180
|
4.3
|
26.2
|
1.0
|
CG
|
A:ASP57
|
4.4
|
39.6
|
1.0
|
O1A
|
A:GCP180
|
4.4
|
29.8
|
1.0
|
CB
|
A:LYS16
|
4.4
|
30.5
|
1.0
|
CA
|
A:THR35
|
4.5
|
61.4
|
1.0
|
O2A
|
A:GCP180
|
4.6
|
41.2
|
1.0
|
PA
|
A:GCP180
|
4.7
|
38.7
|
1.0
|
O
|
A:ASP33
|
4.7
|
66.9
|
1.0
|
CB
|
A:ALA59
|
4.7
|
47.3
|
1.0
|
CA
|
A:ALA59
|
5.0
|
57.7
|
1.0
|
|
Magnesium binding site 2 out
of 4 in 6q21
Go back to
Magnesium Binding Sites List in 6q21
Magnesium binding site 2 out
of 4 in the Molecular Switch For Signal Transduction: Structural Differences Between Active and Inactive Forms of Protooncogenic Ras Proteins
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 2 of Molecular Switch For Signal Transduction: Structural Differences Between Active and Inactive Forms of Protooncogenic Ras Proteins within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mg173
b:30.1
occ:1.00
|
O
|
B:HOH804
|
1.9
|
26.3
|
0.9
|
OG
|
B:SER17
|
2.0
|
23.3
|
1.0
|
O2G
|
B:GCP180
|
2.1
|
34.2
|
1.0
|
O1B
|
B:GCP180
|
2.1
|
32.2
|
1.0
|
O
|
B:HOH803
|
2.3
|
17.3
|
0.9
|
OG1
|
B:THR35
|
2.3
|
30.0
|
1.0
|
CB
|
B:SER17
|
3.1
|
23.6
|
1.0
|
PB
|
B:GCP180
|
3.3
|
30.6
|
1.0
|
CB
|
B:THR35
|
3.4
|
29.1
|
1.0
|
PG
|
B:GCP180
|
3.4
|
33.2
|
1.0
|
C3B
|
B:GCP180
|
3.6
|
38.0
|
1.0
|
N
|
B:SER17
|
3.7
|
25.9
|
1.0
|
OD2
|
B:ASP57
|
3.9
|
27.7
|
1.0
|
CA
|
B:SER17
|
3.9
|
29.1
|
1.0
|
O1A
|
B:GCP180
|
4.1
|
33.6
|
1.0
|
CG2
|
B:THR35
|
4.1
|
19.2
|
1.0
|
OD1
|
B:ASP57
|
4.2
|
33.8
|
1.0
|
O
|
B:HOH904
|
4.2
|
31.1
|
1.0
|
O2B
|
B:GCP180
|
4.3
|
31.4
|
1.0
|
O3A
|
B:GCP180
|
4.3
|
19.2
|
1.0
|
O
|
B:THR58
|
4.3
|
23.4
|
1.0
|
O1G
|
B:GCP180
|
4.4
|
36.3
|
1.0
|
CG
|
B:ASP57
|
4.4
|
21.9
|
1.0
|
O3G
|
B:GCP180
|
4.5
|
29.1
|
1.0
|
PA
|
B:GCP180
|
4.6
|
26.8
|
1.0
|
N
|
B:THR35
|
4.6
|
35.8
|
1.0
|
CB
|
B:LYS16
|
4.6
|
23.5
|
1.0
|
CA
|
B:THR35
|
4.6
|
16.9
|
1.0
|
O2A
|
B:GCP180
|
4.6
|
38.8
|
1.0
|
O
|
B:ASP33
|
4.7
|
39.4
|
1.0
|
C
|
B:PRO34
|
4.7
|
39.8
|
1.0
|
C
|
B:LYS16
|
4.8
|
24.5
|
1.0
|
O
|
B:HOH900
|
4.9
|
38.8
|
0.9
|
O
|
B:PRO34
|
4.9
|
51.2
|
1.0
|
|
Magnesium binding site 3 out
of 4 in 6q21
Go back to
Magnesium Binding Sites List in 6q21
Magnesium binding site 3 out
of 4 in the Molecular Switch For Signal Transduction: Structural Differences Between Active and Inactive Forms of Protooncogenic Ras Proteins
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 3 of Molecular Switch For Signal Transduction: Structural Differences Between Active and Inactive Forms of Protooncogenic Ras Proteins within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Mg173
b:25.7
occ:1.00
|
O3G
|
C:GCP180
|
2.0
|
15.2
|
1.0
|
OG
|
C:SER17
|
2.1
|
15.4
|
1.0
|
O
|
C:HOH807
|
2.1
|
17.8
|
0.9
|
O1B
|
C:GCP180
|
2.2
|
23.8
|
1.0
|
OG1
|
C:THR35
|
2.2
|
17.3
|
1.0
|
O
|
C:HOH806
|
2.3
|
21.4
|
1.0
|
CB
|
C:THR35
|
3.1
|
12.5
|
1.0
|
PB
|
C:GCP180
|
3.2
|
22.2
|
1.0
|
PG
|
C:GCP180
|
3.2
|
17.6
|
1.0
|
CB
|
C:SER17
|
3.3
|
14.4
|
1.0
|
C3B
|
C:GCP180
|
3.5
|
24.7
|
1.0
|
N
|
C:SER17
|
3.9
|
21.0
|
1.0
|
N
|
C:THR35
|
3.9
|
30.9
|
1.0
|
OD1
|
C:ASP57
|
4.1
|
30.2
|
1.0
|
CA
|
C:SER17
|
4.1
|
11.4
|
1.0
|
CG2
|
C:THR35
|
4.1
|
3.6
|
1.0
|
CA
|
C:THR35
|
4.2
|
20.8
|
1.0
|
O1G
|
C:GCP180
|
4.2
|
13.5
|
1.0
|
O3A
|
C:GCP180
|
4.2
|
12.9
|
1.0
|
OD2
|
C:ASP57
|
4.2
|
22.7
|
1.0
|
O2G
|
C:GCP180
|
4.2
|
28.9
|
1.0
|
O1A
|
C:GCP180
|
4.3
|
30.4
|
1.0
|
O
|
C:THR58
|
4.3
|
18.8
|
1.0
|
O2B
|
C:GCP180
|
4.4
|
25.4
|
1.0
|
CG
|
C:ASP57
|
4.5
|
18.3
|
1.0
|
PA
|
C:GCP180
|
4.6
|
19.4
|
1.0
|
CB
|
C:LYS16
|
4.6
|
10.4
|
1.0
|
O
|
C:ASP33
|
4.6
|
31.5
|
1.0
|
O2A
|
C:GCP180
|
4.7
|
16.0
|
1.0
|
O
|
C:HOH910
|
4.8
|
46.3
|
1.0
|
CE
|
C:LYS16
|
4.8
|
20.4
|
1.0
|
NZ
|
C:LYS16
|
4.8
|
28.4
|
1.0
|
C
|
C:LYS16
|
4.9
|
15.5
|
1.0
|
C
|
C:PRO34
|
4.9
|
27.3
|
1.0
|
|
Magnesium binding site 4 out
of 4 in 6q21
Go back to
Magnesium Binding Sites List in 6q21
Magnesium binding site 4 out
of 4 in the Molecular Switch For Signal Transduction: Structural Differences Between Active and Inactive Forms of Protooncogenic Ras Proteins
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 4 of Molecular Switch For Signal Transduction: Structural Differences Between Active and Inactive Forms of Protooncogenic Ras Proteins within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Mg173
b:25.3
occ:1.00
|
O3G
|
D:GCP180
|
1.9
|
37.6
|
1.0
|
O
|
D:HOH810
|
1.9
|
27.8
|
1.0
|
O1B
|
D:GCP180
|
2.0
|
31.2
|
1.0
|
O
|
D:HOH812
|
2.0
|
10.1
|
1.0
|
OG
|
D:SER17
|
2.1
|
25.7
|
1.0
|
O
|
D:HOH809
|
2.1
|
24.0
|
1.0
|
PG
|
D:GCP180
|
3.1
|
45.9
|
1.0
|
CB
|
D:SER17
|
3.2
|
19.2
|
1.0
|
PB
|
D:GCP180
|
3.2
|
31.2
|
1.0
|
C3B
|
D:GCP180
|
3.4
|
38.0
|
1.0
|
O1G
|
D:GCP180
|
3.6
|
46.8
|
1.0
|
N
|
D:SER17
|
3.9
|
26.7
|
1.0
|
OD2
|
D:ASP57
|
3.9
|
33.3
|
1.0
|
O1A
|
D:GCP180
|
4.0
|
22.9
|
1.0
|
CA
|
D:SER17
|
4.0
|
30.4
|
1.0
|
O2B
|
D:GCP180
|
4.1
|
32.4
|
1.0
|
CD2
|
D:TYR32
|
4.2
|
49.9
|
1.0
|
OD1
|
D:ASP57
|
4.2
|
31.8
|
1.0
|
O3A
|
D:GCP180
|
4.3
|
19.6
|
1.0
|
O2G
|
D:GCP180
|
4.4
|
47.2
|
1.0
|
CE2
|
D:TYR32
|
4.4
|
58.2
|
1.0
|
O
|
D:ASP33
|
4.5
|
55.9
|
1.0
|
CG
|
D:ASP57
|
4.5
|
22.0
|
1.0
|
O
|
D:THR58
|
4.5
|
24.7
|
1.0
|
CA
|
D:PRO34
|
4.6
|
53.7
|
1.0
|
PA
|
D:GCP180
|
4.6
|
22.1
|
1.0
|
C
|
D:ASP33
|
4.6
|
63.0
|
1.0
|
N
|
D:PRO34
|
4.7
|
60.0
|
1.0
|
CB
|
D:LYS16
|
4.8
|
16.9
|
1.0
|
O2A
|
D:GCP180
|
4.8
|
32.7
|
1.0
|
O
|
D:HOH813
|
4.8
|
59.2
|
0.9
|
CE
|
D:LYS16
|
4.9
|
29.7
|
1.0
|
C
|
D:LYS16
|
4.9
|
35.5
|
1.0
|
O
|
D:HOH872
|
5.0
|
44.5
|
0.9
|
NZ
|
D:LYS16
|
5.0
|
33.6
|
1.0
|
|
Reference:
M.V.Milburn,
L.Tong,
A.M.Devos,
A.Brunger,
Z.Yamaizumi,
S.Nishimura,
S.H.Kim.
Molecular Switch For Signal Transduction: Structural Differences Between Active and Inactive Forms of Protooncogenic Ras Proteins. Science V. 247 939 1990.
ISSN: ISSN 0036-8075
PubMed: 2406906
Page generated: Tue Oct 1 15:22:40 2024
|