Magnesium in PDB 6r4g: Crystal Structure of Human Gfat-1 in Complex with Udp-Glcnac

All present enzymatic activity of Crystal Structure of Human Gfat-1 in Complex with Udp-Glcnac:
2.6.1.16;

The structure of Crystal Structure of Human Gfat-1 in Complex with Udp-Glcnac, PDB code: 6r4g was solved by S.Ruegenberg, M.Horn, C.Pichlo, K.Allmeroth, U.Baumann, M.S.Denzel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.36 / 2.50
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	152.432, 152.432, 169.268, 90.00, 90.00, 90.00
R / Rfree (%)	19 / 21.7

The binding sites of Magnesium atom in the Crystal Structure of Human Gfat-1 in Complex with Udp-Glcnac (pdb code 6r4g). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Human Gfat-1 in Complex with Udp-Glcnac, PDB code: 6r4g:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Human Gfat-1 in Complex with Udp-Glcnac


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Human Gfat-1 in Complex with Udp-Glcnac within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg702 b:42.1 occ:1.00 O A:THR458 2.4 56.3 1.0 O2 A:UD1701 2.5 52.2 1.0 O A:SER455 2.6 59.9 1.0 O A:ARG456 2.8 54.0 1.0 HA A:ARG456 3.3 65.6 1.0 C A:ARG456 3.4 54.1 1.0 C2 A:UD1701 3.4 53.1 1.0 C A:THR458 3.6 52.9 1.0 C A:SER455 3.8 57.9 1.0 H1B A:UD1701 3.8 68.2 1.0 CA A:ARG456 3.8 54.7 1.0 HG1 A:THR458 3.9 61.9 1.0 OG1 A:THR458 3.9 51.5 1.0 H A:THR458 3.9 64.9 1.0 HN3 A:UD1701 4.0 61.7 1.0 N A:THR458 4.0 54.1 1.0 N3 A:UD1701 4.1 51.4 1.0 O A:ASP459 4.2 55.3 1.0 N A:ARG456 4.3 52.5 1.0 HA A:CYS460 4.3 63.3 1.0 CA A:THR458 4.3 52.3 1.0 C A:ASP459 4.3 50.6 1.0 N A:GLU457 4.4 54.3 1.0 N1 A:UD1701 4.4 55.1 1.0 C1B A:UD1701 4.5 56.8 1.0 C A:GLU457 4.6 51.8 1.0 N A:ASP459 4.6 47.7 1.0 HA A:ASP459 4.6 61.3 1.0 CB A:THR458 4.7 50.7 1.0 N A:CYS460 4.7 53.0 1.0 CA A:ASP459 4.8 51.1 1.0 CA A:CYS460 4.9 52.7 1.0 H A:GLU457 4.9 65.2 1.0 HB A:THR458 4.9 60.8 1.0 CA A:GLU457 4.9 54.6 1.0 HA A:SER455 4.9 67.7 1.0 HA A:GLU457 5.0 65.5 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Human Gfat-1 in Complex with Udp-Glcnac


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Human Gfat-1 in Complex with Udp-Glcnac within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg702 b:50.0 occ:1.00 O2 B:UD1701 2.4 75.9 1.0 O B:THR458 2.5 64.5 1.0 O B:SER455 2.6 66.5 1.0 O B:ARG456 2.8 65.7 1.0 C2 B:UD1701 3.3 78.1 1.0 HA B:ARG456 3.4 82.5 1.0 C B:ARG456 3.5 64.8 1.0 H1B B:UD1701 3.6 90.8 1.0 C B:THR458 3.7 61.5 1.0 C B:SER455 3.8 67.3 1.0 CA B:ARG456 3.9 68.7 1.0 OG1 B:THR458 3.9 57.4 1.0 HG1 B:THR458 3.9 68.8 1.0 H B:THR458 3.9 71.6 1.0 HN3 B:UD1701 4.0 96.0 1.0 N3 B:UD1701 4.1 80.0 1.0 N B:THR458 4.1 59.7 1.0 O B:ASP459 4.2 66.1 1.0 N1 B:UD1701 4.3 79.4 1.0 HA B:CYS460 4.3 81.0 1.0 N B:ARG456 4.3 65.5 1.0 C B:ASP459 4.4 64.7 1.0 C1B B:UD1701 4.4 75.7 1.0 CA B:THR458 4.4 58.0 1.0 N B:GLU457 4.5 67.2 1.0 HA B:ASP459 4.7 76.3 1.0 N B:ASP459 4.7 58.5 1.0 CB B:THR458 4.7 56.7 1.0 N B:CYS460 4.7 67.2 1.0 C B:GLU457 4.7 61.9 1.0 CA B:ASP459 4.8 63.5 1.0 CA B:CYS460 4.9 67.5 1.0 O4B B:UD1701 4.9 73.8 1.0 HA B:SER455 4.9 77.6 1.0 HB B:THR458 4.9 68.1 1.0

S.Ruegenberg, M.Horn, C.Pichlo, K.Allmeroth, U.Baumann, M.S.Denzel. Loss of Gfat-1 Feedback Regulation Activates the Hexosamine Pathway That Modulates Protein Homeostasis To Be Published.