Magnesium in PDB 6svm: Crystal Structure of Human Gfat-1 in Complex with Glucose-6-Phosphate, L-Glu, and Udp-Galnac

All present enzymatic activity of Crystal Structure of Human Gfat-1 in Complex with Glucose-6-Phosphate, L-Glu, and Udp-Galnac:
2.6.1.16;

The structure of Crystal Structure of Human Gfat-1 in Complex with Glucose-6-Phosphate, L-Glu, and Udp-Galnac, PDB code: 6svm was solved by S.Ruegenberg, M.Horn, C.Pichlo, K.Allmeroth, U.Baumann, M.S.Denzel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.15 / 2.48
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	151.995, 151.995, 165.803, 90.00, 90.00, 90.00
R / Rfree (%)	18.9 / 22.2

The binding sites of Magnesium atom in the Crystal Structure of Human Gfat-1 in Complex with Glucose-6-Phosphate, L-Glu, and Udp-Galnac (pdb code 6svm). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Human Gfat-1 in Complex with Glucose-6-Phosphate, L-Glu, and Udp-Galnac, PDB code: 6svm:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Human Gfat-1 in Complex with Glucose-6-Phosphate, L-Glu, and Udp-Galnac


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Human Gfat-1 in Complex with Glucose-6-Phosphate, L-Glu, and Udp-Galnac within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg704 b:44.1 occ:1.00 O A:SER455 2.5 50.5 1.0 O A:THR458 2.6 53.0 1.0 O A:ARG456 2.7 55.7 1.0 O2 A:UD2703 2.7 52.1 1.0 HA A:ARG456 3.2 61.5 1.0 C A:ARG456 3.3 51.6 1.0 C2 A:UD2703 3.6 54.5 1.0 C A:SER455 3.6 50.0 1.0 CA A:ARG456 3.7 51.2 1.0 C A:THR458 3.7 51.8 1.0 H1B A:UD2703 3.8 68.8 1.0 H A:THR458 3.9 59.6 1.0 HG1 A:THR458 3.9 64.6 1.0 OG1 A:THR458 4.0 53.8 1.0 HN3 A:UD2703 4.0 67.0 1.0 N A:THR458 4.1 49.6 1.0 N A:ARG456 4.1 49.3 1.0 N3 A:UD2703 4.2 55.8 1.0 HA A:CYS460 4.4 58.0 1.0 N A:GLU457 4.4 49.2 1.0 CA A:THR458 4.4 50.4 1.0 O A:ASP459 4.5 59.9 1.0 C A:ASP459 4.5 53.6 1.0 N1 A:UD2703 4.5 55.8 1.0 C1B A:UD2703 4.6 57.3 1.0 N A:CYS460 4.7 49.7 1.0 C A:GLU457 4.7 49.3 1.0 CB A:THR458 4.8 50.1 1.0 N A:ASP459 4.8 49.7 1.0 HA A:SER455 4.8 64.4 1.0 HA A:ASP459 4.8 62.3 1.0 CA A:SER455 4.9 53.6 1.0 H A:GLU457 4.9 59.1 1.0 CA A:CYS460 5.0 48.3 1.0 H A:ARG456 5.0 59.3 1.0 CA A:ASP459 5.0 51.8 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Human Gfat-1 in Complex with Glucose-6-Phosphate, L-Glu, and Udp-Galnac


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Human Gfat-1 in Complex with Glucose-6-Phosphate, L-Glu, and Udp-Galnac within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg703 b:59.3 occ:1.00 O2 B:UD2702 2.4 64.0 1.0 O B:SER455 2.5 65.5 1.0 O B:THR458 2.7 62.6 1.0 O B:ARG456 2.8 61.6 1.0 HA B:ARG456 3.2 76.2 1.0 C B:ARG456 3.4 63.0 1.0 C2 B:UD2702 3.4 70.0 1.0 C B:SER455 3.6 65.0 1.0 H1B B:UD2702 3.6 84.9 1.0 CA B:ARG456 3.7 63.5 1.0 C B:THR458 3.8 60.3 1.0 H B:THR458 3.9 70.0 1.0 HN3 B:UD2702 3.9 83.8 1.0 HG1 B:THR458 4.0 70.0 1.0 OG1 B:THR458 4.0 58.2 1.0 N B:ARG456 4.1 63.2 1.0 N3 B:UD2702 4.1 69.8 1.0 N B:THR458 4.2 58.3 1.0 N1 B:UD2702 4.4 71.9 1.0 HA B:CYS460 4.4 72.0 1.0 N B:GLU457 4.4 60.8 1.0 C1B B:UD2702 4.5 70.7 1.0 CA B:THR458 4.5 58.4 1.0 O B:ASP459 4.6 62.8 1.0 C B:ASP459 4.6 61.9 1.0 HA B:SER455 4.8 82.4 1.0 C B:GLU457 4.8 59.6 1.0 N B:CYS460 4.8 60.5 1.0 CB B:THR458 4.8 57.6 1.0 HA B:ASP459 4.8 76.2 1.0 N B:ASP459 4.8 62.0 1.0 CA B:SER455 4.9 68.6 1.0 H B:GLU457 5.0 73.0 1.0 H B:ARG456 5.0 75.9 1.0

S.Ruegenberg, M.Horn, C.Pichlo, K.Allmeroth, U.Baumann, M.S.Denzel. Loss of Gfat-1 Feedback Regulation Activates the Hexosamine Pathway That Modulates Protein Homeostasis To Be Published.