Magnesium in PDB 6svp: Crystal Structure of Human Gfat-1 in Complex with Glucose-6-Phosphate, L-Glu, and Udp-Glcnac

All present enzymatic activity of Crystal Structure of Human Gfat-1 in Complex with Glucose-6-Phosphate, L-Glu, and Udp-Glcnac:
2.6.1.16;

The structure of Crystal Structure of Human Gfat-1 in Complex with Glucose-6-Phosphate, L-Glu, and Udp-Glcnac, PDB code: 6svp was solved by S.Ruegenberg, M.Horn, C.Pichlo, K.Allmeroth, U.Baumann, M.S.Denzel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.36 / 2.53
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	152.628, 152.628, 166.526, 90.00, 90.00, 90.00
R / Rfree (%)	19.7 / 22.4

The binding sites of Magnesium atom in the Crystal Structure of Human Gfat-1 in Complex with Glucose-6-Phosphate, L-Glu, and Udp-Glcnac (pdb code 6svp). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Human Gfat-1 in Complex with Glucose-6-Phosphate, L-Glu, and Udp-Glcnac, PDB code: 6svp:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Human Gfat-1 in Complex with Glucose-6-Phosphate, L-Glu, and Udp-Glcnac


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Human Gfat-1 in Complex with Glucose-6-Phosphate, L-Glu, and Udp-Glcnac within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg702 b:41.9 occ:1.00 O A:ARG456 2.6 57.0 1.0 O A:THR458 2.6 53.0 1.0 O A:SER455 2.6 49.8 1.0 O2 A:UD1701 2.7 54.4 1.0 HA A:ARG456 3.1 70.7 1.0 C A:ARG456 3.3 56.5 1.0 C2 A:UD1701 3.5 53.8 1.0 CA A:ARG456 3.6 58.9 1.0 H1B A:UD1701 3.7 65.0 1.0 C A:SER455 3.7 54.0 1.0 C A:THR458 3.8 52.1 1.0 H A:THR458 3.9 63.1 1.0 HG1 A:THR458 3.9 63.1 1.0 OG1 A:THR458 4.0 52.5 1.0 N A:THR458 4.1 52.5 1.0 N A:ARG456 4.2 55.7 1.0 HN3 A:UD1701 4.2 64.3 1.0 N3 A:UD1701 4.3 53.6 1.0 N1 A:UD1701 4.4 55.6 1.0 N A:GLU457 4.4 53.0 1.0 C1B A:UD1701 4.4 54.1 1.0 CA A:THR458 4.4 52.8 1.0 HA A:CYS460 4.4 60.3 1.0 O A:ASP459 4.5 59.9 1.0 C A:ASP459 4.6 54.3 1.0 C A:GLU457 4.7 50.9 1.0 CB A:THR458 4.8 51.0 1.0 N A:CYS460 4.8 51.2 1.0 N A:ASP459 4.8 49.7 1.0 HA A:ASP459 4.9 58.4 1.0 O4B A:UD1701 5.0 54.3 1.0 CB A:ARG456 5.0 65.1 1.0 H A:GLU457 5.0 63.7 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Human Gfat-1 in Complex with Glucose-6-Phosphate, L-Glu, and Udp-Glcnac


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Human Gfat-1 in Complex with Glucose-6-Phosphate, L-Glu, and Udp-Glcnac within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg702 b:54.8 occ:1.00 O2 B:UD1701 2.4 66.0 1.0 O B:THR458 2.5 61.7 1.0 O B:SER455 2.6 63.8 1.0 O B:ARG456 2.8 65.3 1.0 C2 B:UD1701 3.3 71.2 1.0 HA B:ARG456 3.3 79.9 1.0 C B:ARG456 3.5 66.1 1.0 H1B B:UD1701 3.6 88.8 1.0 C B:THR458 3.7 58.3 1.0 C B:SER455 3.8 63.9 1.0 CA B:ARG456 3.8 66.6 1.0 OG1 B:THR458 3.9 57.4 1.0 HG1 B:THR458 3.9 68.8 1.0 H B:THR458 4.0 68.2 1.0 HN3 B:UD1701 4.0 87.7 1.0 N3 B:UD1701 4.1 73.1 1.0 N B:THR458 4.2 56.8 1.0 O B:ASP459 4.2 68.0 1.0 N1 B:UD1701 4.2 73.8 1.0 N B:ARG456 4.3 64.8 1.0 C1B B:UD1701 4.3 74.0 1.0 HA B:CYS460 4.3 73.4 1.0 C B:ASP459 4.4 63.2 1.0 CA B:THR458 4.4 55.5 1.0 N B:GLU457 4.5 63.9 1.0 N B:ASP459 4.7 59.0 1.0 HA B:ASP459 4.7 74.6 1.0 CB B:THR458 4.7 52.3 1.0 C B:GLU457 4.7 59.8 1.0 N B:CYS460 4.8 60.9 1.0 CA B:ASP459 4.9 62.2 1.0 O4B B:UD1701 4.9 73.0 1.0 HA B:SER455 4.9 75.4 1.0 CA B:CYS460 5.0 61.1 1.0 HB B:THR458 5.0 62.8 1.0

S.Ruegenberg, M.Horn, C.Pichlo, K.Allmeroth, U.Baumann, M.S.Denzel. Loss of Gfat-1 Feedback Regulation Activates the Hexosamine Pathway That Modulates Protein Homeostasis To Be Published.