Magnesium in PDB 6tmu: Crystal Structure of the Chaperonin GP146 From the Bacteriophage El 2 (Pseudomonas Aeruginosa) in Presence of Atp-Befx, Crystal Form II

The structure of Crystal Structure of the Chaperonin GP146 From the Bacteriophage El 2 (Pseudomonas Aeruginosa) in Presence of Atp-Befx, Crystal Form II, PDB code: 6tmu was solved by A.Bracher, S.S.Paul, H.Wang, N.Wischnewski, F.U.Hartl, M.Hayer-Hartl, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 3.54
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	145.516, 151.461, 261.347, 90.00, 90.00, 90.00
R / Rfree (%)	25 / 27.5

The structure of Crystal Structure of the Chaperonin GP146 From the Bacteriophage El 2 (Pseudomonas Aeruginosa) in Presence of Atp-Befx, Crystal Form II also contains other interesting chemical elements:

Potassium

(K)

4 atoms

The binding sites of Magnesium atom in the Crystal Structure of the Chaperonin GP146 From the Bacteriophage El 2 (Pseudomonas Aeruginosa) in Presence of Atp-Befx, Crystal Form II (pdb code 6tmu). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Crystal Structure of the Chaperonin GP146 From the Bacteriophage El 2 (Pseudomonas Aeruginosa) in Presence of Atp-Befx, Crystal Form II, PDB code: 6tmu:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Crystal Structure of the Chaperonin GP146 From the Bacteriophage El 2 (Pseudomonas Aeruginosa) in Presence of Atp-Befx, Crystal Form II


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Chaperonin GP146 From the Bacteriophage El 2 (Pseudomonas Aeruginosa) in Presence of Atp-Befx, Crystal Form II within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg601 b:0.7 occ:1.00 O1B A:ATP603 2.2 0.0 1.0 O2A A:ATP603 2.5 0.8 1.0 OD2 A:ASP86 3.5 0.9 1.0 O3G A:ATP603 3.5 0.4 1.0 C5' A:ATP603 3.5 0.3 1.0 PB A:ATP603 3.6 0.4 1.0 PA A:ATP603 3.7 0.8 1.0 OD1 A:ASP86 3.8 0.3 1.0 O5' A:ATP603 4.0 0.0 1.0 CG A:ASP86 4.1 0.3 1.0 O3A A:ATP603 4.1 0.8 1.0 O3B A:ATP603 4.4 0.1 1.0 PG A:ATP603 4.7 0.4 1.0 O2B A:ATP603 4.8 0.4 1.0 C4' A:ATP603 4.9 0.5 1.0

Magnesium binding site 2 out of 4 in the Crystal Structure of the Chaperonin GP146 From the Bacteriophage El 2 (Pseudomonas Aeruginosa) in Presence of Atp-Befx, Crystal Form II


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Chaperonin GP146 From the Bacteriophage El 2 (Pseudomonas Aeruginosa) in Presence of Atp-Befx, Crystal Form II within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg601 b:0.5 occ:1.00 O2A C:ATP603 2.1 0.3 1.0 O1B C:ATP603 2.3 0.2 1.0 O3G C:ATP603 2.6 0.4 1.0 OD1 C:ASP86 3.1 0.3 1.0 OD2 C:ASP86 3.4 0.9 1.0 PA C:ATP603 3.4 0.2 1.0 PB C:ATP603 3.5 0.4 1.0 CG C:ASP86 3.7 0.4 1.0 O3A C:ATP603 3.7 0.9 1.0 PG C:ATP603 3.9 0.8 1.0 O3B C:ATP603 3.9 0.2 1.0 C5' C:ATP603 4.0 0.6 1.0 O5' C:ATP603 4.1 0.5 1.0 O1A C:ATP603 4.6 0.5 1.0 O2G C:ATP603 4.8 0.9 1.0 O2B C:ATP603 4.8 0.1 1.0 O1G C:ATP603 4.9 0.3 1.0

Magnesium binding site 3 out of 4 in the Crystal Structure of the Chaperonin GP146 From the Bacteriophage El 2 (Pseudomonas Aeruginosa) in Presence of Atp-Befx, Crystal Form II


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of the Chaperonin GP146 From the Bacteriophage El 2 (Pseudomonas Aeruginosa) in Presence of Atp-Befx, Crystal Form II within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg601 b:0.5 occ:1.00 O3G E:ATP603 2.1 1.0 1.0 O2A E:ATP603 2.4 0.7 1.0 OD1 E:ASP86 2.7 0.3 1.0 O1B E:ATP603 2.9 0.6 1.0 OD2 E:ASP86 3.4 0.9 1.0 CG E:ASP86 3.4 0.9 1.0 PG E:ATP603 3.5 0.8 1.0 PA E:ATP603 3.7 0.1 1.0 PB E:ATP603 3.7 0.1 1.0 O3B E:ATP603 3.8 0.2 1.0 O3A E:ATP603 3.9 1.0 1.0 O2G E:ATP603 4.4 0.8 1.0 O1G E:ATP603 4.5 0.7 1.0 O1A E:ATP603 4.7 0.3 1.0 O5' E:ATP603 4.8 0.3 1.0 C5' E:ATP603 4.8 0.8 1.0 CB E:ASP86 4.9 0.7 1.0

Magnesium binding site 4 out of 4 in the Crystal Structure of the Chaperonin GP146 From the Bacteriophage El 2 (Pseudomonas Aeruginosa) in Presence of Atp-Befx, Crystal Form II


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Crystal Structure of the Chaperonin GP146 From the Bacteriophage El 2 (Pseudomonas Aeruginosa) in Presence of Atp-Befx, Crystal Form II within 5.0Å range: probe atom residue distance (Å) B Occ G:Mg601 b:0.3 occ:1.00 O2A G:ATP603 1.9 0.2 1.0 O1B G:ATP603 2.8 0.0 1.0 O3G G:ATP603 3.0 0.5 1.0 PA G:ATP603 3.4 0.6 1.0 OD1 G:ASP86 3.6 0.6 1.0 OD2 G:ASP86 3.7 0.7 1.0 C5' G:ATP603 3.8 0.5 1.0 PB G:ATP603 3.9 0.6 1.0 O3A G:ATP603 4.0 0.2 1.0 CG G:ASP86 4.1 0.9 1.0 O5' G:ATP603 4.1 0.4 1.0 PG G:ATP603 4.3 0.8 1.0 O3B G:ATP603 4.4 0.2 1.0 O1A G:ATP603 4.5 0.6 1.0

A.Bracher, S.S.Paul, H.Wang, N.Wischnewski, F.U.Hartl, M.Hayer-Hartl. Structure and Conformational Cycle of A Bacteriophage-Encoded Chaperonin Plos One 2020.
ISSN: ESSN 1932-6203