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Magnesium in PDB 6uok: Y271G Dna Polymerase Beta Substrate Complex with Templating Cytosine and Incoming R8-Oxo-Gtp

Enzymatic activity of Y271G Dna Polymerase Beta Substrate Complex with Templating Cytosine and Incoming R8-Oxo-Gtp

All present enzymatic activity of Y271G Dna Polymerase Beta Substrate Complex with Templating Cytosine and Incoming R8-Oxo-Gtp:
2.7.7.7;

Protein crystallography data

The structure of Y271G Dna Polymerase Beta Substrate Complex with Templating Cytosine and Incoming R8-Oxo-Gtp, PDB code: 6uok was solved by M.R.Smith, B.D.Freudenthal, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 24.14 / 2.55
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 54.687, 79.957, 100.832, 90.00, 97.64, 90.00
R / Rfree (%) 22.4 / 27.9

Other elements in 6uok:

The structure of Y271G Dna Polymerase Beta Substrate Complex with Templating Cytosine and Incoming R8-Oxo-Gtp also contains other interesting chemical elements:

Manganese (Mn) 6 atoms
Chlorine (Cl) 1 atom
Sodium (Na) 6 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Y271G Dna Polymerase Beta Substrate Complex with Templating Cytosine and Incoming R8-Oxo-Gtp (pdb code 6uok). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Y271G Dna Polymerase Beta Substrate Complex with Templating Cytosine and Incoming R8-Oxo-Gtp, PDB code: 6uok:

Magnesium binding site 1 out of 1 in 6uok

Go back to Magnesium Binding Sites List in 6uok
Magnesium binding site 1 out of 1 in the Y271G Dna Polymerase Beta Substrate Complex with Templating Cytosine and Incoming R8-Oxo-Gtp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Y271G Dna Polymerase Beta Substrate Complex with Templating Cytosine and Incoming R8-Oxo-Gtp within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Mg101

b:31.5
occ:1.00
N7 D:DG4 2.2 28.4 1.0
C8 D:DG4 3.2 29.3 1.0
C5 D:DG4 3.2 27.0 1.0
O6 D:DG4 3.4 30.1 1.0
C6 D:DG4 3.6 31.9 1.0
N9 D:DG4 4.3 28.8 1.0
C4 D:DG4 4.4 25.5 1.0
O6 D:DG5 4.4 34.0 1.0
N4 T:DC1 4.4 44.0 1.0
C2' D:DC3 4.6 33.9 1.0
C6 D:DC3 4.8 22.5 1.0
C5 D:DC3 5.0 18.4 1.0

Reference:

M.R.Smith, K.S.Alnajjar, N.M.Hoitsma, J.B.Sweasy, B.D.Freudenthal. Molecular Characterization of Oxidized Ribonucleotide Insertion By Human Dna Polymerase Beta J.Biol.Chem. 2020.
ISSN: ESSN 1083-351X
Page generated: Tue Oct 1 21:09:42 2024

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