Magnesium in PDB 6wrf: Clpx-Clpp Complex Bound to Gfp-Ssra, Recognition Complex

All present enzymatic activity of Clpx-Clpp Complex Bound to Gfp-Ssra, Recognition Complex:
3.4.21.92;

The binding sites of Magnesium atom in the Clpx-Clpp Complex Bound to Gfp-Ssra, Recognition Complex (pdb code 6wrf). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 5 binding sites of Magnesium where determined in the Clpx-Clpp Complex Bound to Gfp-Ssra, Recognition Complex, PDB code: 6wrf:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5;

Magnesium binding site 1 out of 5 in the Clpx-Clpp Complex Bound to Gfp-Ssra, Recognition Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Clpx-Clpp Complex Bound to Gfp-Ssra, Recognition Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg502 b:30.0 occ:1.00 HG1 B:THR126 1.6 33.8 1.0 OG1 B:THR126 2.0 33.8 1.0 O2G B:AGS501 2.0 32.0 1.0 O2B B:AGS501 2.0 32.0 1.0 OD2 B:ASP184 3.0 28.2 1.0 PG B:AGS501 3.1 32.0 1.0 S1G B:AGS501 3.4 32.0 1.0 PB B:AGS501 3.4 32.0 1.0 CB B:THR126 3.4 33.8 1.0 OD1 B:ASP184 3.4 28.2 1.0 H B:THR126 3.5 33.8 1.0 CG B:ASP184 3.6 28.2 1.0 HB B:THR126 3.7 33.8 1.0 O3B B:AGS501 3.7 32.0 1.0 HE2 B:LYS125 3.8 26.4 1.0 HH22 C:ARG307 3.8 27.9 1.0 HG21 B:THR126 3.9 33.8 1.0 HB2 B:LYS125 4.0 26.4 1.0 O1B B:AGS501 4.1 32.0 1.0 HH B:TYR182 4.1 27.3 1.0 CG2 B:THR126 4.1 33.8 1.0 N B:THR126 4.1 33.8 1.0 OE2 B:GLU185 4.1 30.3 1.0 O1A B:AGS501 4.2 32.0 1.0 HG23 B:THR126 4.3 33.8 1.0 CA B:THR126 4.3 33.8 1.0 O3G B:AGS501 4.4 32.0 1.0 O3A B:AGS501 4.5 32.0 1.0 HA B:THR126 4.5 33.8 1.0 HH12 C:ARG307 4.5 27.9 1.0 OE1 C:GLU216 4.6 30.7 1.0 OH B:TYR182 4.6 27.3 1.0 NH2 C:ARG307 4.7 27.9 1.0 HH11 B:ARG370 4.7 30.4 1.0 CE B:LYS125 4.7 26.4 1.0 HD3 C:LYS213 4.7 31.4 1.0 HZ3 B:LYS125 4.7 26.4 1.0 HZ1 B:LYS125 4.8 26.4 1.0 PA B:AGS501 4.8 32.0 1.0 CD B:GLU185 5.0 30.3 1.0 CB B:LYS125 5.0 26.4 1.0 HD2 C:LYS213 5.0 31.4 1.0 HH12 B:ARG370 5.0 30.4 1.0 NZ B:LYS125 5.0 26.4 1.0

Magnesium binding site 2 out of 5 in the Clpx-Clpp Complex Bound to Gfp-Ssra, Recognition Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Clpx-Clpp Complex Bound to Gfp-Ssra, Recognition Complex within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg502 b:30.0 occ:1.00 HG1 C:THR126 1.6 39.9 1.0 OG1 C:THR126 2.0 39.9 1.0 O2G C:AGS501 2.0 47.0 1.0 O2B C:AGS501 2.4 47.0 1.0 OD2 C:ASP184 2.4 27.9 1.0 CG C:ASP184 3.2 27.9 1.0 OD1 C:ASP184 3.2 27.9 1.0 CB C:THR126 3.4 39.9 1.0 PG C:AGS501 3.5 47.0 1.0 H C:THR126 3.7 39.9 1.0 HG21 C:THR126 3.7 39.9 1.0 HB C:THR126 3.7 39.9 1.0 PB C:AGS501 3.8 47.0 1.0 CG2 C:THR126 4.0 39.9 1.0 OE2 C:GLU185 4.0 28.8 1.0 O3B C:AGS501 4.1 47.0 1.0 HG23 C:THR126 4.1 39.9 1.0 HE2 C:LYS125 4.1 26.9 1.0 HH12 D:ARG307 4.2 28.9 1.0 HB2 C:LYS125 4.2 26.9 1.0 N C:THR126 4.3 39.9 1.0 HH C:TYR182 4.3 26.3 1.0 CA C:THR126 4.3 39.9 1.0 HH22 D:ARG307 4.4 28.9 1.0 S1G C:AGS501 4.4 47.0 1.0 HZ2 D:LYS213 4.4 31.3 1.0 O3G C:AGS501 4.5 47.0 1.0 HA C:THR126 4.5 39.9 1.0 HZ3 D:LYS213 4.6 31.3 1.0 CB C:ASP184 4.6 27.9 1.0 O1A C:AGS501 4.6 47.0 1.0 O1B C:AGS501 4.7 47.0 1.0 HG2 C:GLU185 4.7 28.8 1.0 CD C:GLU185 4.7 28.8 1.0 O3A C:AGS501 4.7 47.0 1.0 OH C:TYR182 4.8 26.3 1.0 HE2 C:TYR182 4.8 26.3 1.0 HH12 C:ARG370 4.8 32.0 1.0 H C:GLU185 4.8 28.8 1.0 HB3 C:ASP184 4.8 27.9 1.0 HG22 C:THR126 4.9 39.9 1.0 NZ D:LYS213 4.9 31.3 1.0 HB2 C:ASP184 4.9 27.9 1.0 NH1 D:ARG307 5.0 28.9 1.0

Magnesium binding site 3 out of 5 in the Clpx-Clpp Complex Bound to Gfp-Ssra, Recognition Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Clpx-Clpp Complex Bound to Gfp-Ssra, Recognition Complex within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg502 b:30.0 occ:1.00 HG1 D:THR126 1.6 34.6 1.0 OG1 D:THR126 2.0 34.6 1.0 O2G D:AGS501 2.0 47.0 1.0 O2B D:AGS501 2.5 47.0 1.0 OD2 D:ASP184 2.6 29.5 1.0 OD1 D:ASP184 3.2 29.5 1.0 CG D:ASP184 3.3 29.5 1.0 CB D:THR126 3.4 34.6 1.0 PG D:AGS501 3.5 47.0 1.0 HG21 D:THR126 3.7 34.6 1.0 H D:THR126 3.8 34.6 1.0 HB D:THR126 3.8 34.6 1.0 PB D:AGS501 3.9 47.0 1.0 OE2 D:GLU185 3.9 30.6 1.0 CG2 D:THR126 4.0 34.6 1.0 HG23 D:THR126 4.0 34.6 1.0 OE1 E:GLU216 4.1 34.8 1.0 HE2 D:LYS125 4.2 28.9 1.0 O3B D:AGS501 4.2 47.0 1.0 HH22 E:ARG307 4.3 30.7 1.0 HD3 E:LYS213 4.3 32.9 1.0 HB2 D:LYS125 4.3 28.9 1.0 N D:THR126 4.3 34.6 1.0 CA D:THR126 4.3 34.6 1.0 HH21 E:ARG307 4.4 30.7 1.0 OE2 E:GLU216 4.4 34.8 1.0 O3G D:AGS501 4.5 47.0 1.0 S1G D:AGS501 4.5 47.0 1.0 HA D:THR126 4.5 34.6 1.0 O1A D:AGS501 4.5 47.0 1.0 O1B D:AGS501 4.6 47.0 1.0 NH2 E:ARG307 4.6 30.7 1.0 CD D:GLU185 4.6 30.6 1.0 OH D:TYR182 4.6 24.8 1.0 CD E:GLU216 4.7 34.8 1.0 HH12 D:ARG370 4.7 26.7 1.0 CB D:ASP184 4.7 29.5 1.0 H D:GLU185 4.7 30.6 1.0 HG2 D:GLU185 4.9 30.6 1.0 HG22 D:THR126 4.9 34.6 1.0 O3A D:AGS501 4.9 47.0 1.0 HE2 D:TYR182 5.0 24.8 1.0 HB3 D:ASP184 5.0 29.5 1.0 HH D:TYR182 5.0 24.8 1.0 HZ3 D:LYS125 5.0 28.9 1.0

Magnesium binding site 4 out of 5 in the Clpx-Clpp Complex Bound to Gfp-Ssra, Recognition Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Clpx-Clpp Complex Bound to Gfp-Ssra, Recognition Complex within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg502 b:30.0 occ:1.00 O2G E:AGS501 2.0 46.9 1.0 OD2 E:ASP184 2.0 38.9 1.0 OD1 E:ASP184 2.1 38.9 1.0 CG E:ASP184 2.3 38.9 1.0 HG1 E:THR126 2.7 37.7 1.0 OG1 E:THR126 3.1 37.7 1.0 PG E:AGS501 3.3 46.9 1.0 O2B E:AGS501 3.3 46.9 1.0 HE2 E:LYS125 3.3 36.6 1.0 HH E:TYR182 3.4 33.2 1.0 S1G E:AGS501 3.6 46.9 1.0 H E:GLU185 3.8 42.4 1.0 CB E:ASP184 3.9 38.9 1.0 OH E:TYR182 4.1 33.2 1.0 HB2 E:LYS125 4.1 36.6 1.0 O3B E:AGS501 4.1 46.9 1.0 HG2 E:GLU185 4.2 42.4 1.0 HB2 E:ASP184 4.2 38.9 1.0 CE E:LYS125 4.2 36.6 1.0 HE2 E:TYR182 4.2 33.2 1.0 H E:THR126 4.3 37.7 1.0 HB3 E:ASP184 4.3 38.9 1.0 OE1 E:GLU185 4.3 42.4 1.0 PB E:AGS501 4.3 46.9 1.0 O3G E:AGS501 4.4 46.9 1.0 HA E:ASP184 4.4 38.9 1.0 CD E:GLU185 4.5 42.4 1.0 CB E:THR126 4.5 37.7 1.0 N E:GLU185 4.5 42.4 1.0 HE3 E:LYS125 4.5 36.6 1.0 HZ3 E:LYS125 4.6 36.6 1.0 HB3 E:LYS125 4.7 36.6 1.0 CA E:ASP184 4.7 38.9 1.0 HZ2 E:LYS125 4.8 36.6 1.0 N E:THR126 4.8 37.7 1.0 HB E:THR126 4.8 37.7 1.0 NZ E:LYS125 4.8 36.6 1.0 O E:GLY248 4.8 31.3 1.0 CG E:GLU185 4.8 42.4 1.0 CB E:LYS125 4.8 36.6 1.0 OE2 E:GLU185 4.9 42.4 1.0 O1B E:AGS501 5.0 46.9 1.0 CE2 E:TYR182 5.0 33.2 1.0

Magnesium binding site 5 out of 5 in the Clpx-Clpp Complex Bound to Gfp-Ssra, Recognition Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Clpx-Clpp Complex Bound to Gfp-Ssra, Recognition Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg502 b:30.0 occ:1.00 OD2 A:ASP184 2.0 44.6 1.0 HG1 A:THR126 2.0 42.1 1.0 OG1 A:THR126 2.0 42.1 1.0 OD1 A:ASP184 2.3 44.6 1.0 S1G A:AGS501 2.4 43.7 1.0 CG A:ASP184 2.4 44.6 1.0 O2B A:AGS501 3.3 43.7 1.0 CB A:THR126 3.4 42.1 1.0 OH A:TYR182 3.5 48.0 1.0 HH A:TYR182 3.7 48.0 1.0 H A:THR126 3.7 42.1 1.0 HG21 A:THR126 3.7 42.1 1.0 OE2 A:GLU185 3.8 42.2 1.0 HE2 A:LYS125 3.9 38.2 1.0 CG2 A:THR126 3.9 42.1 1.0 CB A:ASP184 3.9 44.6 1.0 HG23 A:THR126 3.9 42.1 1.0 HB A:THR126 4.0 42.1 1.0 N A:THR126 4.0 42.1 1.0 HB2 A:LYS125 4.0 38.2 1.0 HA A:THR126 4.1 42.1 1.0 CA A:THR126 4.1 42.1 1.0 HB3 A:LYS125 4.2 38.2 1.0 PG A:AGS501 4.2 43.7 1.0 HB2 A:ASP184 4.3 44.6 1.0 HB3 A:ASP184 4.3 44.6 1.0 H A:GLU185 4.3 42.2 1.0 CD A:GLU185 4.3 42.2 1.0 PB A:AGS501 4.5 43.7 1.0 HG2 A:GLU185 4.5 42.2 1.0 HE2 A:TYR182 4.5 48.0 1.0 CB A:LYS125 4.6 38.2 1.0 HA A:ASP184 4.6 44.6 1.0 O1B A:AGS501 4.6 43.7 1.0 CZ A:TYR182 4.7 48.0 1.0 HA2 A:GLY249 4.7 42.5 1.0 O3G A:AGS501 4.8 43.7 1.0 OE1 A:GLU185 4.8 42.2 1.0 CE A:LYS125 4.9 38.2 1.0 CA A:ASP184 4.9 44.6 1.0 HG22 A:THR126 4.9 42.1 1.0 C A:LYS125 4.9 38.2 1.0 O3B A:AGS501 4.9 43.7 1.0 N A:GLU185 4.9 42.2 1.0

X.Fei, T.A.Bell, S.R.Barkow, T.A.Baker, R.T.Sauer. Structural Basis of Clpxp Recognition and Unfolding of Ssra-Tagged Substrates. Elife V. 9 2020.
ISSN: ESSN 2050-084X
PubMed: 33089779
DOI: 10.7554/ELIFE.61496