Magnesium in PDB 6z7z: Porcine Insulin in Complex with the Analytical Antibody Oxi-005 Fab

The structure of Porcine Insulin in Complex with the Analytical Antibody Oxi-005 Fab, PDB code: 6z7z was solved by E.Johansson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.99 / 2.40
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	109.67, 171.55, 83.17, 90, 135.89, 90
R / Rfree (%)	21 / 25.9

The binding sites of Magnesium atom in the Porcine Insulin in Complex with the Analytical Antibody Oxi-005 Fab (pdb code 6z7z). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Porcine Insulin in Complex with the Analytical Antibody Oxi-005 Fab, PDB code: 6z7z:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Porcine Insulin in Complex with the Analytical Antibody Oxi-005 Fab


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Porcine Insulin in Complex with the Analytical Antibody Oxi-005 Fab within 5.0Å range: probe atom residue distance (Å) B Occ F:Mg101 b:33.8 occ:1.00 O F:HOH206 3.0 41.1 1.0 N F:CYS7 3.3 40.0 1.0 N F:LEU6 3.4 41.0 1.0 N F:VAL12 3.5 36.4 1.0 N F:LEU11 3.6 45.4 1.0 CB F:CYS7 3.7 39.7 1.0 CG2 F:VAL12 3.7 35.1 1.0 CB F:HIS5 3.7 44.6 1.0 O F:HOH202 3.7 50.6 1.0 CB F:VAL12 3.9 38.9 1.0 CA F:CYS7 4.0 32.8 1.0 CA F:HIS5 4.0 48.7 1.0 SG F:CYS7 4.1 35.2 1.0 C F:HIS5 4.1 47.2 1.0 CA F:LEU6 4.2 40.3 1.0 C F:LEU6 4.2 40.9 1.0 C F:HIS10 4.2 42.5 1.0 CB F:LEU11 4.3 40.9 1.0 O F:CYS7 4.3 46.5 1.0 CA F:LEU11 4.3 40.2 1.0 CA F:HIS10 4.3 39.3 1.0 CA F:VAL12 4.4 33.9 1.0 CB F:LEU6 4.4 40.6 1.0 C F:LEU11 4.4 38.6 1.0 C F:CYS7 4.6 42.3 1.0 CG F:HIS5 4.8 50.4 1.0 N F:HIS10 4.9 37.1 1.0 ND1 F:HIS5 4.9 48.2 1.0

Magnesium binding site 2 out of 2 in the Porcine Insulin in Complex with the Analytical Antibody Oxi-005 Fab


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Porcine Insulin in Complex with the Analytical Antibody Oxi-005 Fab within 5.0Å range: probe atom residue distance (Å) B Occ H:Mg101 b:33.6 occ:1.00 N H:VAL12 3.4 41.2 1.0 N H:CYS7 3.4 37.0 1.0 N H:LEU11 3.4 41.4 1.0 CG2 H:VAL12 3.6 41.8 1.0 N H:LEU6 3.6 45.5 1.0 CB H:CYS7 3.7 42.3 1.0 CB H:HIS5 3.7 46.0 1.0 CB H:VAL12 3.9 39.5 1.0 C H:HIS10 4.1 44.2 1.0 CA H:CYS7 4.1 38.8 1.0 CA H:LEU11 4.1 40.6 1.0 CB H:LEU11 4.1 44.4 1.0 CA H:HIS5 4.2 47.3 1.0 SG H:CYS7 4.2 41.3 1.0 CA H:HIS10 4.2 39.6 1.0 O H:CYS7 4.2 40.9 1.0 C H:LEU11 4.3 35.7 1.0 CA H:VAL12 4.3 39.5 1.0 C H:HIS5 4.3 45.1 1.0 CA H:LEU6 4.3 39.8 1.0 C H:LEU6 4.4 35.9 1.0 CB H:LEU6 4.6 29.9 1.0 C H:CYS7 4.6 42.8 1.0 CG H:HIS5 4.7 54.3 1.0 N H:HIS10 4.8 34.7 1.0 CD2 H:HIS5 4.9 52.6 1.0 O H:HIS10 5.0 43.0 1.0

E.Johansson, X.Wu, B.Yu, Z.Yang, Z.Cao, C.Wiberg, C.B.Jeppesen, F.Poulsen. Insulin Binding to the Analytical Antibody Sandwich Pair Oxi-005 and Hui-018: Epitope Mapping and Binding Properties. Protein Sci. 2020.
ISSN: ESSN 1469-896X
PubMed: 33277949
DOI: 10.1002/PRO.4009