Magnesium in PDB 7ax1: Crystal Structure of the Human CCR4-CAF1 Complex

All present enzymatic activity of Crystal Structure of the Human CCR4-CAF1 Complex:
3.1.13.4;

The structure of Crystal Structure of the Human CCR4-CAF1 Complex, PDB code: 7ax1 was solved by Y.Chen, E.Khazina, O.Weichenrieder, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	71.72 / 3.30
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	149.85, 63.26, 113.07, 90, 106.81, 90
R / Rfree (%)	24.8 / 27.6

The binding sites of Magnesium atom in the Crystal Structure of the Human CCR4-CAF1 Complex (pdb code 7ax1). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Human CCR4-CAF1 Complex, PDB code: 7ax1:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Human CCR4-CAF1 Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Human CCR4-CAF1 Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1001 b:121.0 occ:1.00 OE2 A:GLU240 2.0 107.0 1.0 HE1 A:HIS531 2.6 146.9 1.0 CD A:GLU240 3.0 100.2 1.0 OE1 A:GLU240 3.3 94.4 1.0 OD2 A:ASP530 3.5 92.4 1.0 CE1 A:HIS531 3.5 122.4 1.0 HD21 A:ASN195 3.6 116.1 1.0 HD13 A:LEU197 3.6 114.0 1.0 HD12 A:LEU197 4.0 114.0 1.0 ND2 A:ASN195 4.1 96.7 1.0 OD1 A:ASP530 4.2 96.3 1.0 OD1 A:ASN195 4.2 96.5 1.0 HH A:TYR207 4.2 117.2 1.0 HE2 A:HIS361 4.2 113.5 1.0 CD1 A:LEU197 4.2 95.0 1.0 CG A:ASP530 4.2 98.8 1.0 OH A:TYR207 4.3 97.7 1.0 HD1 A:HIS531 4.3 133.5 1.0 ND1 A:HIS531 4.3 111.3 1.0 HA A:LEU197 4.4 123.1 1.0 CG A:GLU240 4.4 95.3 1.0 NE2 A:HIS531 4.5 105.3 1.0 CG A:ASN195 4.5 97.0 1.0 HG3 A:GLU240 4.5 114.3 1.0 HE1 A:HIS361 4.5 116.0 1.0 HD11 A:LEU197 4.6 114.0 1.0 HD22 A:ASN195 4.7 116.1 1.0 NE2 A:HIS361 4.8 94.6 1.0 HG2 A:GLU240 4.8 114.3 1.0 CE1 A:HIS361 5.0 96.7 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Human CCR4-CAF1 Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Human CCR4-CAF1 Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1001 b:158.1 occ:1.00 OE2 B:GLU42 2.2 173.8 1.0 OD2 B:ASP40 2.7 163.4 1.0 OD1 B:ASP40 2.8 156.5 1.0 CD B:GLU42 3.1 177.4 1.0 CG B:ASP40 3.1 157.3 1.0 OD2 B:ASP230 3.4 185.3 1.0 OE1 B:GLU42 3.6 165.8 1.0 O B:THR41 3.7 154.8 1.0 HG2 B:GLU42 3.9 208.1 1.0 CG B:GLU42 4.1 173.4 1.0 HB3 B:ASP230 4.1 210.1 1.0 HB2 B:ASP230 4.2 210.1 1.0 CG B:ASP230 4.3 184.1 1.0 H B:THR41 4.4 199.6 1.0 CB B:ASP230 4.4 175.0 1.0 C B:THR41 4.4 156.7 1.0 HA B:GLU42 4.5 186.7 1.0 HA B:ALA227 4.6 212.0 1.0 CB B:ASP40 4.6 156.6 1.0 HG3 B:GLU42 4.6 208.1 1.0 N B:THR41 4.8 166.3 1.0 HB3 B:ASP40 4.9 187.9 1.0 HB2 B:ASP40 5.0 187.9 1.0

Y.Chen, E.Khazina, E.Izaurralde, O.Weichenrieder. Crystal Structure and Functional Properties of the Human CCR4-CAF1 Deadenylase Complex Nucleic Acids Res. 2021.
ISSN: ESSN 1362-4962
DOI: 10.1093/NAR/GKAB414