Magnesium in PDB 7bdi: Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Atpgammas

All present enzymatic activity of Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Atpgammas:
3.6.4.13;

The structure of Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Atpgammas, PDB code: 7bdi was solved by K.Vester, K.F.Santos, E.Absmeier, M.C.Wahl, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.31 / 2.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	99.729, 118.65, 187.202, 90, 90, 90
R / Rfree (%)	23.7 / 29.7

The binding sites of Magnesium atom in the Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Atpgammas (pdb code 7bdi). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Atpgammas, PDB code: 7bdi:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Atpgammas


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Atpgammas within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg2203 b:53.2 occ:1.00 OD1 B:ASP1454 2.2 31.3 1.0 O3G B:AGS2202 2.2 45.5 1.0 O2G B:AGS2202 2.4 50.9 1.0 OE2 B:GLU1455 2.6 33.7 1.0 NZ B:LYS1356 2.8 44.1 1.0 PG B:AGS2202 2.8 55.9 1.0 CG B:ASP1454 3.1 32.2 1.0 CD B:GLU1455 3.2 39.8 1.0 CG B:GLU1455 3.2 40.4 1.0 OD2 B:ASP1454 3.5 36.9 1.0 N B:GLU1455 3.6 33.4 1.0 O3B B:AGS2202 3.8 51.8 1.0 O B:HOH2331 4.0 28.9 1.0 CA B:GLU1455 4.1 35.0 1.0 CB B:GLU1455 4.2 36.2 1.0 CB B:ASP1454 4.2 31.0 1.0 CE B:LYS1356 4.2 38.2 1.0 OE1 B:GLU1455 4.2 45.5 1.0 S1G B:AGS2202 4.4 61.8 1.0 C B:ASP1454 4.5 35.3 1.0 CB B:LEU1490 4.6 41.6 1.0 CA B:ASP1454 4.6 36.5 1.0 O B:LEU1490 4.7 43.6 1.0 CB B:LYS1356 4.8 39.4 1.0 O1B B:AGS2202 4.8 35.4 1.0 PB B:AGS2202 4.8 37.5 1.0 C B:LEU1490 4.9 40.2 1.0 CD B:LYS1356 5.0 32.8 1.0

Magnesium binding site 2 out of 2 in the Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Atpgammas


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Atpgammas within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg2204 b:46.7 occ:1.00 OE1 B:GLU616 2.1 36.1 1.0 O3G B:AGS2201 2.1 43.1 1.0 O2G B:AGS2201 2.2 39.7 1.0 PG B:AGS2201 2.6 55.6 1.0 OD1 B:ASP615 2.7 40.0 1.0 CD B:GLU616 2.8 37.7 1.0 O B:HOH2336 3.3 38.7 1.0 CG B:GLU616 3.4 37.4 1.0 CG B:ASP615 3.6 35.9 1.0 OE2 B:GLU616 3.6 45.0 1.0 O3B B:AGS2201 3.7 49.2 1.0 OD2 B:ASP615 3.7 47.0 1.0 CE B:LYS509 3.8 35.3 1.0 N B:GLU616 4.0 33.1 1.0 O1B B:AGS2201 4.1 42.2 1.0 S1G B:AGS2201 4.2 77.9 1.0 NZ B:LYS509 4.3 28.6 1.0 CA B:GLU616 4.4 34.5 1.0 PB B:AGS2201 4.4 36.3 1.0 CB B:GLU616 4.5 30.7 1.0 CB B:LEU651 4.6 28.2 1.0 O2B B:AGS2201 4.8 38.0 1.0 CB B:ASP615 4.9 32.5 1.0 O B:HOH2312 4.9 35.4 1.0 O B:LEU651 4.9 34.0 1.0 C B:ASP615 5.0 26.6 1.0

E.Absmeier, K.Vester, T.Ghane, D.Burakovskiy, P.Milon, P.Imhof, M.V.Rodnina, K.F.Santos, M.C.Wahl. Long-Range Allostery Mediates Cooperative Adenine Nucleotide Binding By the SKI2-Like Rna Helicase BRR2. J.Biol.Chem. 00829 2021.
ISSN: ESSN 1083-351X
PubMed: 34048711
DOI: 10.1016/J.JBC.2021.100829