Magnesium in PDB 7bdk: Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Adp

All present enzymatic activity of Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Adp:
3.6.4.13;

The structure of Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Adp, PDB code: 7bdk was solved by K.Vester, K.F.Santos, E.Absmeier, M.C.Wahl, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.09 / 2.52
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	99.532, 118.62, 187.15, 90, 90, 90
R / Rfree (%)	22.8 / 28.4

The binding sites of Magnesium atom in the Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Adp (pdb code 7bdk). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Adp, PDB code: 7bdk:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Adp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Adp within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg2203 b:38.1 occ:1.00 O1B B:ADP2201 2.0 37.1 1.0 O B:HOH2373 2.1 31.8 1.0 O B:HOH2399 2.2 34.3 1.0 O B:HOH2336 2.2 33.5 1.0 O B:HOH2412 2.3 41.4 1.0 O B:HOH2354 2.3 34.0 1.0 O2A B:ADP2201 3.2 43.5 1.0 PB B:ADP2201 3.2 43.3 1.0 O3B B:ADP2201 3.4 41.0 1.0 OG1 B:THR510 3.7 34.9 1.0 OD2 B:ASP615 4.0 42.1 1.0 O B:HOH2390 4.0 32.3 1.0 OD1 B:ASP615 4.2 32.6 1.0 O3A B:ADP2201 4.2 42.6 1.0 PA B:ADP2201 4.2 38.4 1.0 O2B B:ADP2201 4.3 39.9 1.0 CG B:ASP615 4.5 36.2 1.0 OE2 B:GLU616 4.6 37.7 1.0 OE1 B:GLU616 4.6 33.8 1.0 CD B:GLU616 4.6 35.2 1.0 CE B:MET551 4.7 41.0 1.0 O1A B:ADP2201 4.7 41.5 1.0

Magnesium binding site 2 out of 2 in the Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Adp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Adp within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg2204 b:47.6 occ:1.00 O B:HOH2419 2.1 35.1 1.0 O B:HOH2323 2.1 41.1 1.0 O B:HOH2375 2.1 41.1 1.0 O B:HOH2342 2.1 38.2 1.0 O1B B:ADP2202 2.4 38.9 1.0 PB B:ADP2202 3.6 38.9 1.0 O2B B:ADP2202 3.7 47.6 1.0 O2A B:ADP2202 3.8 55.2 1.0 OE1 B:GLU1455 3.9 35.6 1.0 OD2 B:ASP1454 3.9 35.7 1.0 CD B:GLU1455 4.0 42.1 1.0 OG1 B:THR1357 4.1 48.3 1.0 OD1 B:ASP1454 4.1 36.2 1.0 CG B:GLU1455 4.3 40.3 1.0 CG B:ASP1454 4.3 35.1 1.0 OE2 B:GLU1455 4.4 42.1 1.0 O3B B:ADP2202 4.6 37.0 1.0 O3A B:ADP2202 4.8 41.3 1.0 O B:HOH2386 4.8 40.9 1.0 PA B:ADP2202 4.9 43.8 1.0

E.Absmeier, K.Vester, T.Ghane, D.Burakovskiy, P.Milon, P.Imhof, M.V.Rodnina, K.F.Santos, M.C.Wahl. Long-Range Allostery Mediates Cooperative Adenine Nucleotide Binding By the SKI2-Like Rna Helicase BRR2. J.Biol.Chem. 00829 2021.
ISSN: ESSN 1083-351X
PubMed: 34048711
DOI: 10.1016/J.JBC.2021.100829