Magnesium in PDB 7bdl: Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Mant-Adp

All present enzymatic activity of Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Mant-Adp:
3.6.4.13;

The structure of Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Mant-Adp, PDB code: 7bdl was solved by K.Vester, K.F.Santos, E.Absmeier, M.C.Wahl, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.44 / 2.69
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	99.934, 118.783, 187.817, 90, 90, 90
R / Rfree (%)	23.2 / 29.2

The binding sites of Magnesium atom in the Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Mant-Adp (pdb code 7bdl). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Mant-Adp, PDB code: 7bdl:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Mant-Adp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Mant-Adp within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg2203 b:34.1 occ:1.00 O B:HOH2350 2.0 37.3 1.0 O B:HOH2467 2.1 40.8 1.0 O B:HOH2329 2.1 24.2 1.0 O2B B:TG82201 2.2 35.3 1.0 O B:HOH2460 2.3 39.6 1.0 O B:HOH2331 2.7 32.4 1.0 PB B:TG82201 3.5 41.4 1.0 O1B B:TG82201 3.7 40.7 1.0 OG1 B:THR510 3.7 27.4 1.0 OD2 B:ASP615 3.8 43.7 1.0 OD1 B:ASP615 3.9 36.7 1.0 O3A B:TG82201 4.3 32.3 1.0 CG B:ASP615 4.3 35.0 1.0 O3B B:TG82201 4.3 36.5 1.0 OE2 B:GLU616 4.3 34.7 1.0 CD B:GLU616 4.3 36.0 1.0 OE1 B:GLU616 4.4 39.0 1.0 O1A B:TG82201 4.7 44.1 1.0 O2A B:TG82201 4.7 32.7 1.0 PA B:TG82201 4.9 33.1 1.0 CG B:GLU616 5.0 32.2 1.0 CE B:LYS509 5.0 28.4 1.0

Magnesium binding site 2 out of 2 in the Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Mant-Adp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Human BRR2 Helicase Region in Complex with C-Tail Deleted JAB1 and Mant-Adp within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg2204 b:38.4 occ:1.00 O3B B:TG82202 2.0 36.2 1.0 O B:HOH2363 2.1 35.4 1.0 O B:HOH2321 2.1 37.5 1.0 O B:HOH2330 2.1 34.5 1.0 O B:HOH2325 2.5 36.4 1.0 O B:HOH2510 2.6 33.6 1.0 O2B B:TG82202 3.0 52.5 1.0 PB B:TG82202 3.1 46.9 1.0 O2A B:TG82202 3.1 43.8 1.0 OG1 B:THR1357 3.8 44.9 1.0 NZ B:LYS1356 4.1 40.3 1.0 O1A B:TG82202 4.1 42.4 1.0 O1B B:TG82202 4.1 42.6 1.0 PA B:TG82202 4.1 43.1 1.0 OE2 B:GLU1455 4.2 35.4 1.0 OD2 B:ASP1454 4.4 28.5 1.0 CD B:GLU1455 4.5 34.4 1.0 OD1 B:ASP1454 4.6 32.0 1.0 O3A B:TG82202 4.6 52.6 1.0 O B:HOH2395 4.8 26.5 1.0 O B:HOH2468 4.8 41.4 1.0 CG B:ASP1454 4.9 32.0 1.0 OE1 B:GLU1455 4.9 39.6 1.0 N B:THR1357 4.9 40.3 1.0

E.Absmeier, K.Vester, T.Ghane, D.Burakovskiy, P.Milon, P.Imhof, M.V.Rodnina, K.F.Santos, M.C.Wahl. Long-Range Allostery Mediates Cooperative Adenine Nucleotide Binding By the SKI2-Like Rna Helicase BRR2. J.Biol.Chem. 00829 2021.
ISSN: ESSN 1083-351X
PubMed: 34048711
DOI: 10.1016/J.JBC.2021.100829