Magnesium in PDB 7btd: Crystal Structure of Rheb Y35N Mutant Bound to Gppnhp

The structure of Crystal Structure of Rheb Y35N Mutant Bound to Gppnhp, PDB code: 7btd was solved by C.Zhang, T.Zhang, J.Ding, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.00
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	97.637, 102.962, 47.715, 90.00, 90.00, 90.00
R / Rfree (%)	16.6 / 20.8

The binding sites of Magnesium atom in the Crystal Structure of Rheb Y35N Mutant Bound to Gppnhp (pdb code 7btd). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Crystal Structure of Rheb Y35N Mutant Bound to Gppnhp, PDB code: 7btd:

Magnesium binding site 1 out of 1 in the Crystal Structure of Rheb Y35N Mutant Bound to Gppnhp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Rheb Y35N Mutant Bound to Gppnhp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg201 b:32.1 occ:1.00 O2B A:GNP202 1.9 25.4 1.0 O2G A:GNP202 2.0 29.2 1.0 OG1 A:THR38 2.1 28.8 1.0 O A:HOH321 2.1 32.2 1.0 O A:HOH319 2.2 33.5 1.0 OG A:SER20 2.2 27.6 1.0 CB A:THR38 3.1 28.9 1.0 PB A:GNP202 3.2 28.9 1.0 PG A:GNP202 3.2 31.5 1.0 CB A:SER20 3.2 27.8 1.0 N3B A:GNP202 3.4 29.1 1.0 N A:THR38 3.8 29.5 1.0 N A:SER20 3.9 27.3 1.0 CA A:THR38 4.1 30.2 1.0 CA A:SER20 4.1 28.3 1.0 OD2 A:ASP60 4.1 33.3 1.0 O2A A:GNP202 4.1 30.6 1.0 O3G A:GNP202 4.1 31.0 1.0 O1B A:GNP202 4.2 27.4 1.0 CG2 A:THR38 4.2 28.9 1.0 O A:HOH324 4.2 33.7 1.0 O3A A:GNP202 4.3 28.9 1.0 O1G A:GNP202 4.3 27.9 1.0 OD1 A:ASP60 4.3 36.7 1.0 O A:ASP36 4.4 33.5 1.0 O A:THR61 4.5 29.0 1.0 PA A:GNP202 4.5 29.9 1.0 CG A:ASP60 4.6 32.6 1.0 O1A A:GNP202 4.6 29.4 1.0 C A:PRO37 4.8 32.7 1.0 CB A:LYS19 4.8 28.6 1.0 C A:LYS19 4.9 28.6 1.0 NZ A:LYS19 5.0 26.2 1.0

C.Zhang, Y.Liu, Y.Zhang, X.Wang, T.Zhang, J.Ding. Molecular Basis For the Functions of Dominantly Active Y35N and Inactive D60K Rheb Mutants in MTORC1 Signaling. J Mol Cell Biol 2020.
ISSN: ESSN 1759-4685
PubMed: 32470140
DOI: 10.1093/JMCB/MJAA025