Magnesium in PDB 7eip: Crystal Structure of Ligand-Free Chondroitin Abc Lyase I

All present enzymatic activity of Crystal Structure of Ligand-Free Chondroitin Abc Lyase I:
4.2.2.20;

The structure of Crystal Structure of Ligand-Free Chondroitin Abc Lyase I, PDB code: 7eip was solved by M.Takashima, A.Miyanaga, T.Eguchi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.47 / 1.88
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	48.944, 94.239, 229.082, 90, 90, 90
R / Rfree (%)	16.9 / 21.3

The binding sites of Magnesium atom in the Crystal Structure of Ligand-Free Chondroitin Abc Lyase I (pdb code 7eip). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Crystal Structure of Ligand-Free Chondroitin Abc Lyase I, PDB code: 7eip:

Magnesium binding site 1 out of 1 in the Crystal Structure of Ligand-Free Chondroitin Abc Lyase I


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Ligand-Free Chondroitin Abc Lyase I within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1103 b:36.5 occ:1.00 O A:MET70 2.0 35.0 1.0 O A:GLN73 2.3 44.2 1.0 OD1 A:ASP211 2.3 31.6 1.0 O A:HIS43 2.4 36.1 1.0 OD2 A:ASP211 2.4 34.5 1.0 CG A:ASP211 2.7 33.3 1.0 C A:MET70 3.2 35.9 1.0 C A:HIS43 3.4 40.0 1.0 O A:HOH1722 3.4 34.0 1.0 C A:GLN73 3.5 40.3 1.0 CA A:HIS43 3.7 38.2 1.0 CB A:HIS43 3.9 38.9 1.0 N A:MET70 3.9 34.1 1.0 CA A:MET70 4.1 34.7 1.0 N A:GLY71 4.1 39.0 1.0 N A:GLN73 4.2 38.8 1.0 CB A:ASP211 4.2 32.0 1.0 CD2 A:PHE44 4.2 40.8 1.0 CA A:GLY71 4.2 40.9 1.0 CB A:SER68 4.3 36.8 1.0 C A:GLY71 4.3 42.9 1.0 O A:GLY71 4.3 43.4 1.0 N A:SER74 4.4 41.7 1.0 CA A:GLN73 4.4 41.6 1.0 CA A:SER74 4.6 43.0 1.0 N A:PHE44 4.6 39.9 1.0 CB A:MET70 4.6 35.6 1.0 N A:ILE69 4.7 34.1 1.0 CB A:PHE44 4.7 41.6 1.0 N A:ASN72 4.9 43.0 1.0 CG A:PHE44 5.0 42.1 1.0

M.Takashima, I.Watanabe, A.Miyanaga, T.Eguchi. Substrate Specificity of Chondroitinase Abc I Based on Analyses of Biochemical Reactions and Crystal Structures in Complex with Disaccharides. Glycobiology 2021.
ISSN: ESSN 1460-2423
PubMed: 34392362
DOI: 10.1093/GLYCOB/CWAB086