Magnesium in PDB 7f1a: Odinarchaeota Tubulin (Odintubulin) H393D Mutant, in A Protofilament Arrangement, Bound 78% Gtp/22% Gdp 1 K+, 1 MG2+

The structure of Odinarchaeota Tubulin (Odintubulin) H393D Mutant, in A Protofilament Arrangement, Bound 78% Gtp/22% Gdp 1 K+, 1 MG2+, PDB code: 7f1a was solved by R.C.Robinson, C.Akil, L.T.Tran, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.20 / 1.90
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	40.416, 92.835, 102.516, 90, 90, 90
R / Rfree (%)	18 / 22

The structure of Odinarchaeota Tubulin (Odintubulin) H393D Mutant, in A Protofilament Arrangement, Bound 78% Gtp/22% Gdp 1 K+, 1 MG2+ also contains other interesting chemical elements:

Potassium

(K)

1 atom

The binding sites of Magnesium atom in the Odinarchaeota Tubulin (Odintubulin) H393D Mutant, in A Protofilament Arrangement, Bound 78% Gtp/22% Gdp 1 K+, 1 MG2+ (pdb code 7f1a). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Odinarchaeota Tubulin (Odintubulin) H393D Mutant, in A Protofilament Arrangement, Bound 78% Gtp/22% Gdp 1 K+, 1 MG2+, PDB code: 7f1a:

Magnesium binding site 1 out of 1 in the Odinarchaeota Tubulin (Odintubulin) H393D Mutant, in A Protofilament Arrangement, Bound 78% Gtp/22% Gdp 1 K+, 1 MG2+


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Odinarchaeota Tubulin (Odintubulin) H393D Mutant, in A Protofilament Arrangement, Bound 78% Gtp/22% Gdp 1 K+, 1 MG2+ within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg503 b:28.5 occ:0.86 O B:HOH675 1.9 34.3 1.0 O B:HOH663 2.0 29.1 1.0 O2G B:GTP501 2.2 28.1 0.8 O B:HOH658 2.2 30.8 1.0 O2B B:GTP501 2.2 26.5 0.8 O1B B:GDP502 2.3 28.2 0.2 PG B:GTP501 3.3 30.6 0.8 PB B:GTP501 3.3 30.1 0.8 K B:K504 3.4 32.3 0.8 PB B:GDP502 3.5 30.0 0.2 O3B B:GTP501 3.6 30.7 0.8 O3G B:GTP501 3.8 28.4 0.8 O3A B:GDP502 3.8 30.4 0.2 CB B:GLN13 3.9 33.5 1.0 O3A B:GTP501 3.9 30.8 0.8 O3B B:GDP502 3.9 30.4 0.2 OE1 B:GLU71 4.1 38.2 1.0 CG B:GLU71 4.1 39.7 1.0 N B:GLN13 4.2 36.6 1.0 OE1 B:GLN13 4.2 35.1 1.0 OD1 B:ASP69 4.3 35.1 1.0 O2A B:GTP501 4.3 27.4 0.8 OD2 B:ASP69 4.3 34.8 1.0 O2A B:GDP502 4.5 28.3 0.2 CD B:GLU71 4.5 38.9 1.0 O1B B:GTP501 4.6 30.9 0.8 O1G B:GTP501 4.6 26.4 0.8 CA B:GLN13 4.6 36.8 1.0 PA B:GTP501 4.6 28.8 0.8 PA B:GDP502 4.7 29.0 0.2 O2B B:GDP502 4.7 30.8 0.2 CG B:ASP69 4.8 34.7 1.0 O B:HOH706 4.9 29.8 1.0

C.Akil, S.Ali, L.T.Tran, J.Gaillard, W.Li, K.Hayashida, M.Hirose, T.Kato, A.Oshima, K.Fujishima, L.Blanchoin, A.Narita, R.C.Robinson. Structure and Dynamics of Odinarchaeota Tubulin and the Implications For Eukaryotic Microtubule Evolution. Sci Adv V. 8 M2225 2022.
ISSN: ESSN 2375-2548
PubMed: 35333570
DOI: 10.1126/SCIADV.ABM2225