Magnesium in PDB 7f1n: Beta-Glucosidase

The structure of Beta-Glucosidase, PDB code: 7f1n was solved by C.Anke, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	111.21 / 2.14
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	76.72, 62.21, 112.45, 90, 98.51, 90
R / Rfree (%)	21 / 25.3

The binding sites of Magnesium atom in the Beta-Glucosidase (pdb code 7f1n). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Beta-Glucosidase, PDB code: 7f1n:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Beta-Glucosidase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Beta-Glucosidase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg601 b:11.4 occ:1.00 OD1 A:ASP258 2.3 22.5 1.0 O A:VAL202 2.3 19.7 1.0 O A:VAL260 2.3 19.8 1.0 O A:GLY199 2.4 22.3 1.0 O A:ALA269 2.5 20.6 1.0 OD1 A:ASP203 2.5 21.7 1.0 OD2 A:ASP258 2.7 20.7 1.0 CG A:ASP258 2.9 24.5 1.0 C A:VAL202 3.4 19.0 1.0 C A:GLY199 3.4 23.7 1.0 C A:VAL260 3.6 21.6 1.0 C A:ALA269 3.6 24.8 1.0 CG A:ASP203 3.7 22.2 1.0 CA A:ASP203 3.8 19.7 1.0 CA A:GLY199 3.9 17.1 1.0 N A:ASP203 4.0 18.8 1.0 CA A:ALA269 4.2 20.3 1.0 N A:ALA262 4.2 26.0 1.0 CB A:ASP203 4.3 16.6 1.0 CB A:ASP258 4.4 20.4 1.0 N A:VAL260 4.4 29.2 1.0 CA A:VAL260 4.4 25.3 1.0 CB A:ALA269 4.5 19.7 1.0 N A:VAL202 4.5 19.6 1.0 N A:ARG261 4.5 22.1 1.0 CA A:VAL202 4.6 18.4 1.0 N A:ASN200 4.6 23.5 1.0 CA A:ARG261 4.6 25.4 1.0 NE1 A:TRP205 4.6 20.0 1.0 N A:ALA270 4.7 16.8 1.0 CB A:VAL260 4.7 24.7 1.0 OD2 A:ASP203 4.7 23.9 1.0 CB A:ALA262 4.8 30.5 1.0 C A:ARG261 4.9 25.1 1.0 C A:ASP203 5.0 20.0 1.0 CA A:ASN200 5.0 24.8 1.0

Magnesium binding site 2 out of 2 in the Beta-Glucosidase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Beta-Glucosidase within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg601 b:9.2 occ:1.00 O B:VAL202 2.3 23.6 1.0 O B:VAL260 2.3 22.4 1.0 O B:ALA269 2.3 19.5 1.0 OD1 B:ASP203 2.4 18.6 1.0 OD1 B:ASP258 2.4 17.4 1.0 O B:GLY199 2.4 19.3 1.0 OD2 B:ASP258 2.6 17.9 1.0 CG B:ASP258 2.8 20.2 1.0 C B:VAL202 3.3 20.4 1.0 C B:GLY199 3.4 21.5 1.0 C B:ALA269 3.5 19.9 1.0 C B:VAL260 3.5 23.5 1.0 CG B:ASP203 3.5 21.0 1.0 CA B:ASP203 3.7 17.9 1.0 CA B:GLY199 3.9 18.8 1.0 N B:ASP203 3.9 19.1 1.0 CA B:ALA269 4.1 22.3 1.0 CB B:ASP203 4.1 21.7 1.0 N B:ALA262 4.2 24.5 1.0 CB B:ASP258 4.3 18.0 1.0 N B:VAL260 4.4 19.9 1.0 CA B:VAL260 4.4 20.6 1.0 N B:ARG261 4.4 19.3 1.0 CA B:ARG261 4.5 24.3 1.0 CB B:ALA269 4.5 22.4 1.0 N B:ALA270 4.5 17.4 1.0 OD2 B:ASP203 4.5 26.0 1.0 N B:VAL202 4.6 23.7 1.0 CA B:VAL202 4.6 18.8 1.0 N B:ASN200 4.6 19.4 1.0 NE1 B:TRP205 4.6 13.4 1.0 CB B:VAL260 4.7 24.7 1.0 CA B:ALA270 4.8 23.0 1.0 C B:ARG261 4.9 29.1 1.0 C B:ASP203 4.9 22.4 1.0 CA B:ASN200 5.0 22.2 1.0 CB B:ALA262 5.0 28.5 1.0

A.Chen, D.Wang, R.Ji, J.Li, S.Gu, R.Tang, C.Ji. Structural and Catalytic Characterization of Tsbgl, A Beta-Glucosidase From Thermofilum Sp. EX4484_79. Front Microbiol V. 12 23678 2021.
ISSN: ESSN 1664-302X
PubMed: 34659150
DOI: 10.3389/FMICB.2021.723678