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Magnesium in PDB 7f4b: The Crystal Structure of the Immature Apo-Enzyme of Homoserine Dehydrogenase From the Hyperthermophilic Archaeon Sulfurisphaera Tokodaii.

Enzymatic activity of The Crystal Structure of the Immature Apo-Enzyme of Homoserine Dehydrogenase From the Hyperthermophilic Archaeon Sulfurisphaera Tokodaii.

All present enzymatic activity of The Crystal Structure of the Immature Apo-Enzyme of Homoserine Dehydrogenase From the Hyperthermophilic Archaeon Sulfurisphaera Tokodaii.:
1.1.1.3;

Protein crystallography data

The structure of The Crystal Structure of the Immature Apo-Enzyme of Homoserine Dehydrogenase From the Hyperthermophilic Archaeon Sulfurisphaera Tokodaii., PDB code: 7f4b was solved by E.Kurihara, T.Kubota, K.Watanabe, K.Ogata, R.Kaneko, T.Oshima, K.Yoshimune, M.Goto, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 48.59 / 2.05
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 57.098, 78.305, 65.551, 90, 106.17, 90
R / Rfree (%) 23.1 / 28.1

Magnesium Binding Sites:

The binding sites of Magnesium atom in the The Crystal Structure of the Immature Apo-Enzyme of Homoserine Dehydrogenase From the Hyperthermophilic Archaeon Sulfurisphaera Tokodaii. (pdb code 7f4b). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the The Crystal Structure of the Immature Apo-Enzyme of Homoserine Dehydrogenase From the Hyperthermophilic Archaeon Sulfurisphaera Tokodaii., PDB code: 7f4b:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 7f4b

Go back to Magnesium Binding Sites List in 7f4b
Magnesium binding site 1 out of 2 in the The Crystal Structure of the Immature Apo-Enzyme of Homoserine Dehydrogenase From the Hyperthermophilic Archaeon Sulfurisphaera Tokodaii.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of The Crystal Structure of the Immature Apo-Enzyme of Homoserine Dehydrogenase From the Hyperthermophilic Archaeon Sulfurisphaera Tokodaii. within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg501

b:41.7
occ:1.00
O A:LEU43 2.1 40.0 1.0
O A:HOH607 2.2 28.6 1.0
O A:HOH619 2.5 34.8 1.0
C A:LEU43 3.3 39.5 1.0
O A:GLY33 3.9 34.7 1.0
CA A:GLN44 3.9 37.4 1.0
N A:GLN44 4.0 36.3 1.0
O A:HOH668 4.3 34.3 1.0
CA A:LEU43 4.4 40.2 1.0
O A:ILE32 4.5 35.5 1.0
CB A:LEU43 4.6 39.5 1.0
C A:GLY33 4.7 35.4 1.0
CG A:GLN44 4.7 37.5 1.0
CB A:GLN44 4.8 37.1 1.0
CA A:GLY33 4.8 35.5 1.0
C A:GLN44 4.9 39.3 1.0

Magnesium binding site 2 out of 2 in 7f4b

Go back to Magnesium Binding Sites List in 7f4b
Magnesium binding site 2 out of 2 in the The Crystal Structure of the Immature Apo-Enzyme of Homoserine Dehydrogenase From the Hyperthermophilic Archaeon Sulfurisphaera Tokodaii.


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of The Crystal Structure of the Immature Apo-Enzyme of Homoserine Dehydrogenase From the Hyperthermophilic Archaeon Sulfurisphaera Tokodaii. within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg502

b:50.2
occ:1.00
O A:HOH662 2.0 43.6 1.0
O A:HOH613 2.1 47.0 1.0
O A:HOH608 2.1 35.4 1.0
O A:HOH602 4.2 31.4 1.0
OE2 A:GLU81 4.2 41.9 1.0
O A:ASN79 4.3 29.6 1.0
CD2 A:LEU84 4.3 31.9 1.0
O A:HOH655 4.4 45.2 1.0
CB A:ASN79 4.8 27.4 1.0

Reference:

T.Kubota, E.Kurihara, K.Watanabe, K.Ogata, R.Kaneko, M.Goto, T.Ohshima, K.Yoshimune. Conformational Changes in the Catalytic Region Are Responsible For Heat-Induced Activation of Hyperthermophilic Homoserine Dehydrogenase. Commun Biol V. 5 704 2022.
ISSN: ESSN 2399-3642
PubMed: 35835834
DOI: 10.1038/S42003-022-03656-7
Page generated: Wed Oct 2 21:10:01 2024

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