Magnesium in PDB 7js3: Structure of the Class II Fructose-1,6-Bisphophatase From Francisella Tularensis

The structure of Structure of the Class II Fructose-1,6-Bisphophatase From Francisella Tularensis, PDB code: 7js3 was solved by C.Abad-Zapatero, N.M.Wolf, F.Movahedzadeh, H.Gutka, A.Selezneva, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	38.11 / 2.40
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	76.296, 100.169, 92.021, 90.00, 90.00, 90.00
R / Rfree (%)	15.3 / 19.3

The binding sites of Magnesium atom in the Structure of the Class II Fructose-1,6-Bisphophatase From Francisella Tularensis (pdb code 7js3). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Structure of the Class II Fructose-1,6-Bisphophatase From Francisella Tularensis, PDB code: 7js3:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Structure of the Class II Fructose-1,6-Bisphophatase From Francisella Tularensis


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of the Class II Fructose-1,6-Bisphophatase From Francisella Tularensis within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg401 b:31.1 occ:1.00 O A:HOH502 2.0 30.7 1.0 OD1 A:ASP116 2.1 36.0 1.0 OE2 A:GLU214 2.1 29.9 1.0 OE1 A:GLU214 2.3 29.7 1.0 CD A:GLU214 2.6 26.2 1.0 CG A:ASP116 2.8 41.1 1.0 OD2 A:ASP116 2.9 43.2 1.0 CA A:GLY211 3.8 29.8 1.0 CG A:GLU214 4.1 26.0 1.0 CD A:PRO213 4.1 30.1 1.0 CB A:ASP116 4.3 38.5 1.0 O A:HOH537 4.5 29.1 1.0 CG A:PRO213 4.5 31.1 1.0 C A:GLY211 4.6 24.9 1.0 N A:GLY211 4.7 27.5 1.0 O A:HOH540 4.8 34.8 1.0 O A:HOH520 4.8 22.6 1.0 CB A:PRO213 4.9 30.3 1.0 CA A:ASP116 4.9 43.6 1.0 N A:ALA212 4.9 23.9 1.0 N A:GLU214 5.0 26.9 1.0

Magnesium binding site 2 out of 4 in the Structure of the Class II Fructose-1,6-Bisphophatase From Francisella Tularensis


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of the Class II Fructose-1,6-Bisphophatase From Francisella Tularensis within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg402 b:27.1 occ:1.00 OD1 A:ASP84 1.9 26.0 1.0 O A:LEU86 2.0 27.1 1.0 O A:HOH532 2.0 32.8 1.0 O A:HOH504 2.0 33.5 1.0 O A:HOH547 2.1 34.9 1.0 O A:HOH540 2.1 34.8 1.0 CG A:ASP84 2.8 27.3 1.0 C A:LEU86 3.1 24.5 1.0 OD2 A:ASP84 3.1 29.7 1.0 N A:LEU86 3.8 23.3 1.0 N A:GLU87 3.9 21.6 1.0 CA A:LEU86 4.0 21.9 1.0 CA A:GLU87 4.0 24.8 1.0 OE2 A:GLU57 4.1 43.6 1.0 CB A:ASP84 4.1 24.8 1.0 OD2 A:ASP33 4.3 44.0 1.0 OD1 A:ASP33 4.5 29.2 1.0 CA A:ASP84 4.5 27.4 1.0 CB A:LEU86 4.5 21.6 1.0 C A:ASP84 4.7 23.4 1.0 N A:PRO85 4.7 26.5 1.0 CD A:PRO85 4.7 27.9 1.0 CG2 A:THR89 4.7 29.6 1.0 CG A:ASP33 4.7 37.2 1.0 CB A:GLU87 5.0 24.2 1.0 O A:HOH537 5.0 29.1 1.0 C A:PRO85 5.0 22.8 1.0 CD A:GLU57 5.0 38.6 1.0 OE1 A:GLU57 5.0 36.9 1.0

Magnesium binding site 3 out of 4 in the Structure of the Class II Fructose-1,6-Bisphophatase From Francisella Tularensis


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Structure of the Class II Fructose-1,6-Bisphophatase From Francisella Tularensis within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg401 b:39.5 occ:1.00 O B:HOH502 2.1 47.4 1.0 O B:HOH554 2.1 47.5 1.0 OD1 B:ASP84 2.1 40.0 1.0 O B:LEU86 2.2 39.6 1.0 CG B:ASP84 3.0 44.9 1.0 OD2 B:ASP84 3.3 48.0 1.0 C B:LEU86 3.3 35.9 1.0 CA B:GLU87 3.9 38.0 1.0 CG2 B:THR89 4.0 44.6 1.0 N B:GLU87 4.0 36.0 1.0 OD2 B:ASP33 4.2 63.7 1.0 N B:GLY88 4.3 36.6 1.0 OD1 B:ASP33 4.3 55.7 1.0 CA B:LEU86 4.4 36.1 1.0 OE2 B:GLU57 4.4 60.6 1.0 CB B:ASP84 4.5 43.4 1.0 N B:LEU86 4.5 38.0 1.0 CG B:ASP33 4.6 53.6 1.0 C B:GLU87 4.6 35.4 1.0 CB B:LEU86 4.9 38.0 1.0 N B:THR89 5.0 39.9 1.0 CB B:GLU87 5.0 36.9 1.0

Magnesium binding site 4 out of 4 in the Structure of the Class II Fructose-1,6-Bisphophatase From Francisella Tularensis


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Structure of the Class II Fructose-1,6-Bisphophatase From Francisella Tularensis within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg401 b:68.6 occ:1.00 OE2 C:GLU87 2.1 74.0 0.7 O C:LEU86 2.1 86.5 1.0 OD2 C:ASP33 3.2 0.2 1.0 C C:LEU86 3.2 84.0 1.0 CD C:GLU87 3.4 78.2 0.7 OD1 C:ASP84 3.4 0.0 1.0 OE1 C:GLU57 3.6 0.3 1.0 CG C:ASP84 3.8 0.4 1.0 CA C:GLU87 3.8 85.7 0.7 CA C:GLU87 3.8 82.6 0.3 N C:GLU87 3.9 81.3 1.0 OE2 C:GLU57 4.0 0.0 1.0 CG C:ASP33 4.0 0.5 1.0 OD1 C:ASP33 4.1 0.7 1.0 OE1 C:GLU87 4.2 74.5 0.7 N C:LEU86 4.2 84.4 1.0 CB C:ASP84 4.2 0.6 1.0 CD C:GLU57 4.2 0.6 1.0 CB C:GLU87 4.3 85.0 0.7 CA C:LEU86 4.3 83.0 1.0 CG C:GLU87 4.4 81.0 0.7 OD2 C:ASP84 4.4 0.2 1.0 CB C:GLU87 4.5 79.4 0.3 CA C:ASP84 4.8 95.4 1.0 C C:ASP84 5.0 95.7 1.0

A.I.Selezneva, H.J.Gutka, N.M.Wolf, F.Qurratulain, F.Movahedzadeh, C.Abad-Zapatero. Structural and Biochemical Characterization of the Class II Fructose-1,6-Bisphosphatase From Francisella Tularensis. Acta Crystallogr.,Sect.F V. 76 524 2020.
ISSN: ESSN 2053-230X
PubMed: 33135671
DOI: 10.1107/S2053230X20013370