Magnesium in PDB 7kt8: Dna Polymerase Mu, 8-Oxodgtp:at Product State Ternary Complex, 50 Mm MG2+ (180MIN)

All present enzymatic activity of Dna Polymerase Mu, 8-Oxodgtp:at Product State Ternary Complex, 50 Mm MG2+ (180MIN):
2.7.7.7;

The structure of Dna Polymerase Mu, 8-Oxodgtp:at Product State Ternary Complex, 50 Mm MG2+ (180MIN), PDB code: 7kt8 was solved by J.A.Jamsen, S.H.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.76 / 1.70
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	59.891, 68.503, 110.793, 90, 90, 90
R / Rfree (%)	16.8 / 19.1

The structure of Dna Polymerase Mu, 8-Oxodgtp:at Product State Ternary Complex, 50 Mm MG2+ (180MIN) also contains other interesting chemical elements:

Sodium	(Na)	1 atom
Chlorine	(Cl)	1 atom

The binding sites of Magnesium atom in the Dna Polymerase Mu, 8-Oxodgtp:at Product State Ternary Complex, 50 Mm MG2+ (180MIN) (pdb code 7kt8). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Dna Polymerase Mu, 8-Oxodgtp:at Product State Ternary Complex, 50 Mm MG2+ (180MIN), PDB code: 7kt8:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Dna Polymerase Mu, 8-Oxodgtp:at Product State Ternary Complex, 50 Mm MG2+ (180MIN)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Dna Polymerase Mu, 8-Oxodgtp:at Product State Ternary Complex, 50 Mm MG2+ (180MIN) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg505 b:26.9 occ:1.00 O21 A:PPV508 2.1 19.6 0.7 OD1 A:ASP330 2.1 28.8 1.0 OD2 A:ASP332 2.1 24.7 1.0 O32 A:PPV508 2.1 26.4 0.6 O A:HOH654 2.1 32.9 1.0 OP1 P:8OG5 2.2 28.8 1.0 P1 A:PPV508 3.0 29.9 0.7 CG A:ASP332 3.1 29.0 1.0 CG A:ASP330 3.1 56.7 1.0 O31 A:PPV508 3.2 35.6 0.7 P2 A:PPV508 3.3 27.2 0.4 OD1 A:ASP332 3.4 24.1 1.0 MG A:MG506 3.5 23.0 1.0 OD2 A:ASP330 3.5 45.1 1.0 OPP A:PPV508 3.6 32.9 0.7 P P:8OG5 3.6 32.6 1.0 O12 A:PPV508 3.9 33.7 0.7 O A:ASP330 4.1 25.6 1.0 O5' P:8OG5 4.1 20.7 1.0 OP2 P:8OG5 4.2 30.8 1.0 O A:HOH619 4.2 21.5 1.0 C5' P:8OG5 4.3 22.6 1.0 N A:GLY320 4.3 20.6 1.0 C A:ASP330 4.3 26.3 1.0 O11 A:PPV508 4.4 24.0 0.8 O A:HOH612 4.4 30.4 1.0 CB A:ASP332 4.4 17.9 1.0 CB A:ASP330 4.5 48.6 1.0 CA A:GLY319 4.5 19.3 1.0 O22 A:PPV508 4.5 26.4 0.4 N A:ASP332 4.8 18.3 1.0 O3' P:DA4 4.8 21.0 1.0 CA A:ASP330 4.8 26.1 1.0 N A:VAL331 4.8 21.6 1.0 N A:ASP330 4.9 21.9 1.0 C A:GLY319 5.0 22.0 1.0 C A:VAL331 5.0 20.5 1.0

Magnesium binding site 2 out of 2 in the Dna Polymerase Mu, 8-Oxodgtp:at Product State Ternary Complex, 50 Mm MG2+ (180MIN)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Dna Polymerase Mu, 8-Oxodgtp:at Product State Ternary Complex, 50 Mm MG2+ (180MIN) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg506 b:23.0 occ:1.00 OD2 A:ASP330 1.8 45.1 1.0 OP1 P:8OG5 2.2 28.8 1.0 OD2 A:ASP418 2.2 23.8 1.0 OD1 A:ASP332 2.2 24.1 1.0 O A:HOH612 2.3 30.4 1.0 O3' P:DA4 2.7 21.0 1.0 P P:8OG5 2.8 32.6 1.0 CG A:ASP330 2.9 56.7 1.0 OD1 A:ASP330 3.2 28.8 1.0 CG A:ASP332 3.2 29.0 1.0 CG A:ASP418 3.2 21.8 1.0 MG A:MG505 3.5 26.9 1.0 OD2 A:ASP332 3.6 24.7 1.0 OP2 P:8OG5 3.6 30.8 1.0 C3' P:DA4 3.8 18.0 1.0 CB A:ASP418 3.9 18.6 1.0 C5' P:DA4 4.0 19.9 1.0 C4' P:DA4 4.0 18.8 1.0 O5' P:8OG5 4.1 20.7 1.0 OD1 A:ASP418 4.2 23.5 1.0 CB A:ASP330 4.2 48.6 1.0 C5' P:8OG5 4.5 22.6 1.0 NH2 A:ARG416 4.5 20.1 1.0 CB A:ASP332 4.5 17.9 1.0 O32 A:PPV508 4.6 26.4 0.6 OP1 P:DA4 4.7 21.7 1.0 CZ3 A:TRP434 4.7 21.3 1.0 O5' P:DA4 4.8 21.1 1.0 O A:VAL331 4.8 19.7 1.0

J.A.Jamsen, A.Sassa, D.D.Shock, W.A.Beard, S.H.Wilson. Dna Polymerase Mu, 8-Oxodgtp:at Product State Ternary Complex, 50 Mm MG2+ (180MIN) To Be Published.