Magnesium in PDB 7m0a: Incomplete in Crystallo Incorporation By Dna Polymerase Lambda Bound to Blunt-Ended Dsb Substrate and Incoming Dttp
Enzymatic activity of Incomplete in Crystallo Incorporation By Dna Polymerase Lambda Bound to Blunt-Ended Dsb Substrate and Incoming Dttp
All present enzymatic activity of Incomplete in Crystallo Incorporation By Dna Polymerase Lambda Bound to Blunt-Ended Dsb Substrate and Incoming Dttp:
2.7.7.7;
Protein crystallography data
The structure of Incomplete in Crystallo Incorporation By Dna Polymerase Lambda Bound to Blunt-Ended Dsb Substrate and Incoming Dttp, PDB code: 7m0a
was solved by
A.M.Kaminski,
K.Bebenek,
L.C.Pedersen,
T.A.Kunkel,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
43.71 /
1.83
|
Space group
|
P 21 21 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
55.768,
59.573,
140.726,
90,
90,
90
|
R / Rfree (%)
|
16.9 /
19.3
|
Other elements in 7m0a:
The structure of Incomplete in Crystallo Incorporation By Dna Polymerase Lambda Bound to Blunt-Ended Dsb Substrate and Incoming Dttp also contains other interesting chemical elements:
Magnesium Binding Sites:
The binding sites of Magnesium atom in the Incomplete in Crystallo Incorporation By Dna Polymerase Lambda Bound to Blunt-Ended Dsb Substrate and Incoming Dttp
(pdb code 7m0a). This binding sites where shown within
5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the
Incomplete in Crystallo Incorporation By Dna Polymerase Lambda Bound to Blunt-Ended Dsb Substrate and Incoming Dttp, PDB code: 7m0a:
Jump to Magnesium binding site number:
1;
2;
Magnesium binding site 1 out
of 2 in 7m0a
Go back to
Magnesium Binding Sites List in 7m0a
Magnesium binding site 1 out
of 2 in the Incomplete in Crystallo Incorporation By Dna Polymerase Lambda Bound to Blunt-Ended Dsb Substrate and Incoming Dttp
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 1 of Incomplete in Crystallo Incorporation By Dna Polymerase Lambda Bound to Blunt-Ended Dsb Substrate and Incoming Dttp within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mg703
b:17.3
occ:0.20
|
CA
|
A:CA704
|
0.0
|
17.0
|
0.8
|
OD1
|
A:ASP429
|
2.0
|
16.8
|
1.0
|
OD2
|
A:ASP427
|
2.0
|
26.8
|
1.0
|
OD2
|
A:ASP490
|
2.1
|
20.2
|
1.0
|
OP1
|
P:DT7
|
2.2
|
14.9
|
0.6
|
O1A
|
A:TTP701
|
2.4
|
15.7
|
0.4
|
O
|
A:HOH822
|
2.6
|
35.0
|
1.0
|
O3'
|
P:DC6
|
2.6
|
22.5
|
0.4
|
O3'
|
P:DC6
|
2.7
|
21.7
|
0.6
|
CG
|
A:ASP427
|
2.9
|
27.3
|
1.0
|
P
|
P:DT7
|
3.0
|
17.5
|
0.6
|
CG
|
A:ASP429
|
3.0
|
17.0
|
1.0
|
OD1
|
A:ASP427
|
3.1
|
18.4
|
1.0
|
CG
|
A:ASP490
|
3.1
|
17.5
|
1.0
|
OD2
|
A:ASP429
|
3.4
|
15.0
|
1.0
|
MG
|
A:MG705
|
3.4
|
16.4
|
1.0
|
PA
|
A:TTP701
|
3.6
|
17.3
|
0.4
|
CB
|
A:ASP490
|
3.6
|
16.1
|
1.0
|
C3'
|
P:DC6
|
3.8
|
21.6
|
0.4
|
C3'
|
P:DC6
|
3.9
|
21.4
|
0.6
|
O5'
|
A:TTP701
|
4.0
|
19.4
|
0.4
|
OP2
|
P:DT7
|
4.1
|
14.8
|
0.6
|
O5'
|
P:DT7
|
4.1
|
19.6
|
0.6
|
O2A
|
A:TTP701
|
4.1
|
15.9
|
0.4
|
OD1
|
A:ASP490
|
4.1
|
17.2
|
1.0
|
C5'
|
P:DT7
|
4.2
|
16.1
|
0.6
|
C4'
|
P:DC6
|
4.2
|
21.2
|
0.6
|
C4'
|
P:DC6
|
4.2
|
21.2
|
0.4
|
CB
|
A:ASP427
|
4.3
|
16.5
|
1.0
|
C5'
|
P:DC6
|
4.3
|
20.2
|
0.4
|
C5'
|
A:TTP701
|
4.3
|
15.7
|
0.4
|
CB
|
A:ASP429
|
4.3
|
14.6
|
1.0
|
C5'
|
P:DC6
|
4.5
|
20.2
|
0.6
|
O
|
A:HOH865
|
4.5
|
37.1
|
1.0
|
NH2
|
A:ARG488
|
4.7
|
17.1
|
1.0
|
O
|
A:VAL428
|
4.7
|
15.6
|
1.0
|
O1G
|
A:TTP701
|
4.7
|
22.5
|
0.4
|
O11
|
A:PPV702
|
4.8
|
22.5
|
0.6
|
CA
|
A:ASP429
|
4.8
|
15.8
|
1.0
|
CA
|
A:ASP490
|
4.9
|
14.1
|
1.0
|
CE1
|
A:PHE506
|
4.9
|
18.9
|
1.0
|
C
|
A:VAL428
|
4.9
|
16.8
|
1.0
|
N
|
A:ASP490
|
4.9
|
14.6
|
1.0
|
O2B
|
A:TTP701
|
4.9
|
19.2
|
0.4
|
C2'
|
P:DC6
|
5.0
|
22.8
|
0.6
|
O3A
|
A:TTP701
|
5.0
|
25.1
|
0.4
|
O5'
|
P:DC6
|
5.0
|
23.4
|
0.4
|
N
|
A:ASP429
|
5.0
|
13.8
|
1.0
|
|
Magnesium binding site 2 out
of 2 in 7m0a
Go back to
Magnesium Binding Sites List in 7m0a
Magnesium binding site 2 out
of 2 in the Incomplete in Crystallo Incorporation By Dna Polymerase Lambda Bound to Blunt-Ended Dsb Substrate and Incoming Dttp
Mono view
Stereo pair view
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 2 of Incomplete in Crystallo Incorporation By Dna Polymerase Lambda Bound to Blunt-Ended Dsb Substrate and Incoming Dttp within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mg705
b:16.4
occ:1.00
|
O2B
|
A:TTP701
|
2.0
|
19.2
|
0.4
|
O1A
|
A:TTP701
|
2.0
|
15.7
|
0.4
|
O12
|
A:PPV702
|
2.0
|
19.2
|
0.6
|
OD2
|
A:ASP429
|
2.1
|
15.0
|
1.0
|
OD1
|
A:ASP427
|
2.1
|
18.4
|
1.0
|
O1G
|
A:TTP701
|
2.1
|
22.5
|
0.4
|
O11
|
A:PPV702
|
2.2
|
22.5
|
0.6
|
OP1
|
P:DT7
|
2.2
|
14.9
|
0.6
|
O
|
A:HOH887
|
2.2
|
20.6
|
1.0
|
PB
|
A:TTP701
|
3.0
|
21.3
|
0.4
|
CG
|
A:ASP429
|
3.1
|
17.0
|
1.0
|
PA
|
A:TTP701
|
3.1
|
17.3
|
0.4
|
P2
|
A:PPV702
|
3.1
|
21.5
|
0.6
|
CG
|
A:ASP427
|
3.2
|
27.3
|
1.0
|
O3A
|
A:TTP701
|
3.2
|
25.1
|
0.4
|
O32
|
A:PPV702
|
3.3
|
25.3
|
0.6
|
PG
|
A:TTP701
|
3.4
|
27.4
|
0.4
|
OD1
|
A:ASP429
|
3.4
|
16.8
|
1.0
|
CA
|
A:CA704
|
3.4
|
17.0
|
0.8
|
P1
|
A:PPV702
|
3.4
|
27.6
|
0.6
|
MG
|
A:MG703
|
3.4
|
17.3
|
0.2
|
O3B
|
A:TTP701
|
3.6
|
27.0
|
0.4
|
P
|
P:DT7
|
3.6
|
17.5
|
0.6
|
OPP
|
A:PPV702
|
3.6
|
27.1
|
0.6
|
OD2
|
A:ASP427
|
3.6
|
26.8
|
1.0
|
O
|
A:HOH865
|
3.9
|
37.1
|
1.0
|
C5'
|
A:TTP701
|
4.0
|
15.7
|
0.4
|
O5'
|
P:DT7
|
4.0
|
19.6
|
0.6
|
O31
|
A:PPV702
|
4.0
|
23.7
|
0.6
|
O5'
|
A:TTP701
|
4.1
|
19.4
|
0.4
|
O
|
A:ASP427
|
4.1
|
16.6
|
1.0
|
O3G
|
A:TTP701
|
4.1
|
23.7
|
0.4
|
C5'
|
P:DT7
|
4.2
|
16.1
|
0.6
|
O2A
|
A:TTP701
|
4.2
|
15.9
|
0.4
|
C
|
A:ASP427
|
4.4
|
17.2
|
1.0
|
CB
|
A:ASP429
|
4.4
|
14.6
|
1.0
|
O1B
|
A:TTP701
|
4.4
|
18.9
|
0.4
|
O
|
A:HOH958
|
4.4
|
19.5
|
1.0
|
OP2
|
P:DT7
|
4.4
|
14.8
|
0.6
|
OG
|
A:SER417
|
4.4
|
21.0
|
1.0
|
N
|
A:SER417
|
4.4
|
13.8
|
1.0
|
CB
|
A:ASP427
|
4.4
|
16.5
|
1.0
|
N
|
A:ASP427
|
4.4
|
14.3
|
1.0
|
O
|
A:HOH822
|
4.4
|
35.0
|
1.0
|
O22
|
A:PPV702
|
4.5
|
19.0
|
0.6
|
CA
|
A:GLY416
|
4.5
|
13.5
|
1.0
|
O2G
|
A:TTP701
|
4.6
|
27.9
|
0.4
|
O21
|
A:PPV702
|
4.6
|
28.0
|
0.6
|
O3'
|
P:DC6
|
4.6
|
21.7
|
0.6
|
CA
|
A:ASP427
|
4.7
|
16.5
|
1.0
|
N
|
A:ASP429
|
4.9
|
13.8
|
1.0
|
O3'
|
P:DC6
|
4.9
|
22.5
|
0.4
|
N
|
A:VAL428
|
5.0
|
14.9
|
1.0
|
|
Reference:
A.M.Kaminski,
K.Bebenek,
T.A.Kunkel,
L.C.Pedersen.
Comprehensive Structural Survey of Dna Double-Strand Break Synapsis By Dna Polymerase Lambda To Be Published.
Page generated: Wed Apr 5 13:17:38 2023
|