Magnesium in PDB 7m0b: Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Bound Mismatched Dsb and Incoming Dumpnpp

All present enzymatic activity of Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Bound Mismatched Dsb and Incoming Dumpnpp:
2.7.7.7;

The structure of Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Bound Mismatched Dsb and Incoming Dumpnpp, PDB code: 7m0b was solved by A.M.Kaminski, K.Bebenek, L.C.Pedersen, T.A.Kunkel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.40 / 2.00
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	56.368, 59.961, 139.406, 90, 90, 90
R / Rfree (%)	17.1 / 20

The structure of Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Bound Mismatched Dsb and Incoming Dumpnpp also contains other interesting chemical elements:

Chlorine	(Cl)	4 atoms
Sodium	(Na)	2 atoms

The binding sites of Magnesium atom in the Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Bound Mismatched Dsb and Incoming Dumpnpp (pdb code 7m0b). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Bound Mismatched Dsb and Incoming Dumpnpp, PDB code: 7m0b:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Bound Mismatched Dsb and Incoming Dumpnpp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Bound Mismatched Dsb and Incoming Dumpnpp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg702 b:17.0 occ:0.64 OD2 A:ASP427 2.0 14.8 1.0 OD1 A:ASP429 2.2 14.2 1.0 OD2 A:ASP490 2.2 16.3 1.0 O3' P:DC6 2.3 17.9 0.6 O A:HOH937 2.3 17.5 1.0 O2A A:DUP701 2.3 8.1 1.0 CG A:ASP427 3.0 16.2 1.0 CG A:ASP490 3.2 19.1 1.0 CG A:ASP429 3.2 13.4 1.0 PA A:DUP701 3.3 12.9 1.0 O3' P:DC6 3.3 16.5 0.4 C3' P:DC6 3.3 15.1 0.4 C3' P:DC6 3.4 14.8 0.6 OD1 A:ASP427 3.4 10.9 1.0 O5' A:DUP701 3.6 13.7 1.0 OD2 A:ASP429 3.6 12.2 1.0 O1A A:DUP701 3.6 14.0 1.0 MG A:MG703 3.7 10.6 1.0 CB A:ASP490 3.8 12.8 1.0 C5' A:DUP701 4.0 12.1 1.0 C4' P:DC6 4.2 15.8 0.6 OD1 A:ASP490 4.2 16.4 1.0 O A:HOH904 4.2 19.1 1.0 C4' P:DC6 4.3 15.8 0.4 C5' P:DC6 4.3 14.7 0.4 C5' P:DC6 4.3 14.7 0.6 CB A:ASP427 4.3 13.5 1.0 C2' P:DC6 4.4 14.9 0.4 O5' P:DC6 4.5 15.4 0.4 NH2 A:ARG488 4.5 17.8 1.0 O5' P:DC6 4.5 15.6 0.6 CB A:ASP429 4.5 13.8 1.0 C2' P:DC6 4.6 14.3 0.6 O3G A:DUP701 4.7 12.4 1.0 N3A A:DUP701 4.8 11.1 1.0 OP1 P:DC6 5.0 16.1 0.6 CE1 A:PHE506 5.0 15.3 1.0 OP1 P:DC6 5.0 16.0 0.4

Magnesium binding site 2 out of 2 in the Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Bound Mismatched Dsb and Incoming Dumpnpp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Pre-Catalytic Quaternary Complex of Dna Polymerase Lambda with Bound Mismatched Dsb and Incoming Dumpnpp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg703 b:10.6 occ:1.00 O2B A:DUP701 2.0 8.5 1.0 O3G A:DUP701 2.0 12.4 1.0 OD1 A:ASP427 2.1 10.9 1.0 O2A A:DUP701 2.1 8.1 1.0 OD2 A:ASP429 2.1 12.2 1.0 O A:HOH913 2.2 12.2 1.0 CG A:ASP429 3.1 13.4 1.0 PB A:DUP701 3.1 13.2 1.0 CG A:ASP427 3.1 16.2 1.0 PA A:DUP701 3.3 12.9 1.0 PG A:DUP701 3.3 14.1 1.0 OD1 A:ASP429 3.5 14.2 1.0 N3A A:DUP701 3.5 11.1 1.0 O3B A:DUP701 3.5 11.8 1.0 OD2 A:ASP427 3.6 14.8 1.0 MG A:MG702 3.7 17.0 0.6 O A:HOH904 3.9 19.1 1.0 O2G A:DUP701 4.0 12.6 1.0 O A:ASP427 4.1 17.0 1.0 C5' A:DUP701 4.1 12.1 1.0 OG A:SER417 4.2 16.4 1.0 O5' A:DUP701 4.2 13.7 1.0 N A:SER417 4.3 9.8 1.0 N A:ASP427 4.3 11.4 1.0 C A:ASP427 4.4 14.5 1.0 O A:HOH853 4.4 12.2 1.0 CB A:ASP427 4.4 13.5 1.0 O1A A:DUP701 4.4 14.0 1.0 O1B A:DUP701 4.4 12.1 1.0 CA A:GLY416 4.4 12.4 1.0 O1G A:DUP701 4.4 12.9 1.0 CB A:ASP429 4.5 13.8 1.0 CA A:ASP427 4.6 14.6 1.0 O A:HOH937 4.6 17.5 1.0 N A:ASP429 4.9 12.1 1.0 C A:GLY416 5.0 12.7 1.0 N A:VAL428 5.0 12.7 1.0 CB A:SER417 5.0 15.9 1.0

A.M.Kaminski, K.Bebenek, T.A.Kunkel, L.C.Pedersen. Comprehensive Structural Survey of Dna Double-Strand Break Synapsis By Dna Polymerase Lambda To Be Published.