Magnesium in PDB 7m7r: Human Dna Pol Eta S113A with Ra-Ended Primer and Dampnpp

All present enzymatic activity of Human Dna Pol Eta S113A with Ra-Ended Primer and Dampnpp:
2.7.7.7;

The structure of Human Dna Pol Eta S113A with Ra-Ended Primer and Dampnpp, PDB code: 7m7r was solved by M.T.Gregory, Y.Gao, W.Yang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	23.37 / 1.81
Space group	P 61
Cell size a, b, c (Å), α, β, γ (°)	98.648, 98.648, 81.744, 90, 90, 120
R / Rfree (%)	18.3 / 22.1

The binding sites of Magnesium atom in the Human Dna Pol Eta S113A with Ra-Ended Primer and Dampnpp (pdb code 7m7r). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Human Dna Pol Eta S113A with Ra-Ended Primer and Dampnpp, PDB code: 7m7r:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Human Dna Pol Eta S113A with Ra-Ended Primer and Dampnpp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Human Dna Pol Eta S113A with Ra-Ended Primer and Dampnpp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg502 b:8.9 occ:1.00 OD1 A:ASP13 2.0 12.1 1.0 OD2 A:ASP115 2.1 7.9 1.0 O1G A:DZ4501 2.1 9.3 1.0 O A:MET14 2.1 7.1 1.0 O1B A:DZ4501 2.1 8.9 1.0 O1A A:DZ4501 2.3 11.1 1.0 CG A:ASP13 3.1 8.8 1.0 PB A:DZ4501 3.1 8.5 1.0 CG A:ASP115 3.2 10.6 1.0 PG A:DZ4501 3.3 11.2 1.0 PA A:DZ4501 3.3 13.3 1.0 C A:MET14 3.3 6.6 1.0 OD2 A:ASP13 3.4 16.4 1.0 N3A A:DZ4501 3.5 10.5 1.0 O3B A:DZ4501 3.5 9.1 1.0 MG A:MG503 3.5 10.1 1.0 OD1 A:ASP115 3.7 9.7 1.0 O A:HOH709 3.8 14.9 1.0 NZ A:LYS231 3.8 6.7 1.0 N A:MET14 3.9 9.5 1.0 O2G A:DZ4501 4.0 9.4 1.0 C5' A:DZ4501 4.1 11.5 1.0 CA A:MET14 4.1 8.2 1.0 O5' A:DZ4501 4.2 11.3 1.0 C A:ASP13 4.2 8.9 1.0 CB A:ASP13 4.3 8.9 1.0 N A:ASP15 4.4 7.9 1.0 CB A:ASP115 4.5 6.2 1.0 O A:HOH700 4.5 18.4 1.0 N A:CYS16 4.5 9.8 1.0 O2B A:DZ4501 4.5 9.4 1.0 O3G A:DZ4501 4.5 9.6 1.0 CA A:ASP15 4.5 8.2 1.0 CE A:LYS231 4.6 13.6 1.0 O2A A:DZ4501 4.6 14.2 1.0 CB A:MET14 4.6 9.5 1.0 O A:ASP13 4.6 9.3 1.0 C A:ASP15 4.7 12.3 1.0 N A:PHE17 4.7 5.8 1.0 CA A:ASP13 4.7 10.7 1.0 CB A:PHE17 4.8 7.3 1.0 O A:ASP115 4.9 10.7 1.0 O3' P:A9 5.0 17.6 1.0

Magnesium binding site 2 out of 2 in the Human Dna Pol Eta S113A with Ra-Ended Primer and Dampnpp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Human Dna Pol Eta S113A with Ra-Ended Primer and Dampnpp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg503 b:10.1 occ:1.00 O3' P:A9 2.1 17.6 1.0 OD2 A:ASP13 2.1 16.4 1.0 OD1 A:ASP115 2.1 9.7 1.0 O A:HOH700 2.1 18.4 1.0 O1A A:DZ4501 2.2 11.1 1.0 OE2 A:GLU116 2.3 19.0 1.0 CG A:ASP115 3.1 10.6 1.0 CG A:ASP13 3.1 8.8 1.0 OD2 A:ASP115 3.3 7.9 1.0 PA A:DZ4501 3.3 13.3 1.0 C3' P:A9 3.4 21.3 1.0 CD A:GLU116 3.4 21.4 1.0 OD1 A:ASP13 3.5 12.1 1.0 MG A:MG502 3.5 8.9 1.0 O2A A:DZ4501 3.7 14.2 1.0 O5' A:DZ4501 3.8 11.3 1.0 O A:HOH709 4.0 14.9 1.0 C4' P:A9 4.0 25.0 1.0 C5' A:DZ4501 4.1 11.5 1.0 CB A:GLU116 4.2 12.7 1.0 OE1 A:GLU116 4.2 22.5 1.0 CG A:GLU116 4.2 15.1 1.0 C5' P:A9 4.3 22.0 1.0 CB A:ASP13 4.4 8.9 1.0 NZ A:LYS224 4.4 21.1 1.0 O2' P:A9 4.5 20.8 1.0 CB A:ASP115 4.5 6.2 1.0 C2' P:A9 4.5 22.4 1.0 C A:ASP115 4.6 11.0 1.0 O A:ASP115 4.6 10.7 1.0 O1G A:DZ4501 4.7 9.3 1.0 N3A A:DZ4501 4.8 10.5 1.0 N A:GLU116 4.8 10.7 1.0 O1B A:DZ4501 4.9 8.9 1.0 CA A:ASP115 5.0 8.4 1.0

M.T.Gregory, Y.Gao, Q.Cui, W.Yang. Multiple Deprotonation Paths of the Nucleophile 3'-Oh in the Dna Synthesis Reaction Proc.Natl.Acad.Sci.Usa 2021.
ISSN: ESSN 1091-6490