Magnesium in PDB 7mxj: Crystal Structure of the S/T Protein Kinase Pkng From Corynebacterium Glutamicum (Residues 130-433) in Complex with Amp-Pnp, Isoform 1

The structure of Crystal Structure of the S/T Protein Kinase Pkng From Corynebacterium Glutamicum (Residues 130-433) in Complex with Amp-Pnp, Isoform 1, PDB code: 7mxj was solved by M.N.Lisa, P.M.Alzari, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.52 / 1.92
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	37.616, 55.936, 123.945, 90, 90, 90
R / Rfree (%)	19.9 / 22.5

The binding sites of Magnesium atom in the Crystal Structure of the S/T Protein Kinase Pkng From Corynebacterium Glutamicum (Residues 130-433) in Complex with Amp-Pnp, Isoform 1 (pdb code 7mxj). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the S/T Protein Kinase Pkng From Corynebacterium Glutamicum (Residues 130-433) in Complex with Amp-Pnp, Isoform 1, PDB code: 7mxj:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the S/T Protein Kinase Pkng From Corynebacterium Glutamicum (Residues 130-433) in Complex with Amp-Pnp, Isoform 1


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the S/T Protein Kinase Pkng From Corynebacterium Glutamicum (Residues 130-433) in Complex with Amp-Pnp, Isoform 1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg501 b:24.7 occ:1.00 OD2 A:ASP301 2.0 19.6 1.0 OD2 A:ASP318 2.0 23.6 1.0 O A:HOH750 2.0 25.2 1.0 O1G A:MAP502 2.1 26.6 1.0 O A:HOH719 2.2 23.6 1.0 O A:HOH624 2.2 29.5 1.0 CG A:ASP318 3.1 26.2 1.0 CG A:ASP301 3.1 20.5 1.0 PG A:MAP502 3.4 25.0 1.0 OD1 A:ASP318 3.6 26.8 1.0 CB A:ASP301 3.7 18.8 1.0 O A:HOH687 3.7 24.5 1.0 O2G A:MAP502 3.9 25.9 1.0 O A:HOH798 3.9 36.8 1.0 O A:HOH804 4.0 36.4 1.0 N3B A:MAP502 4.1 30.9 1.0 OD1 A:ASP301 4.2 18.1 1.0 ND2 A:ASN306 4.2 18.3 1.0 CB A:ASP318 4.3 25.0 1.0 CE A:LYS303 4.5 21.8 1.0 O3G A:MAP502 4.6 30.0 1.0 O A:HOH668 4.6 28.9 1.0 O1A A:MAP502 4.6 33.3 1.0 CB A:ALA321 4.6 25.8 1.0 O A:TYR332 4.7 23.1 1.0 NZ A:LYS303 4.7 23.8 1.0 O A:ASP301 4.9 17.6 1.0 O2A A:MAP502 4.9 28.5 1.0 OG1 A:THR334 5.0 20.2 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the S/T Protein Kinase Pkng From Corynebacterium Glutamicum (Residues 130-433) in Complex with Amp-Pnp, Isoform 1


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the S/T Protein Kinase Pkng From Corynebacterium Glutamicum (Residues 130-433) in Complex with Amp-Pnp, Isoform 1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg502 b:25.3 occ:1.00 MG A:MAP502 0.0 25.3 1.0 O1B A:MAP502 1.9 28.4 1.0 O2G A:MAP502 1.9 25.9 1.0 O2A A:MAP502 2.0 28.5 1.0 O A:HOH699 2.1 29.4 1.0 OE2 A:GLU305 2.1 25.7 1.0 O A:HOH646 2.3 27.6 1.0 PB A:MAP502 2.8 31.2 1.0 PA A:MAP502 3.1 30.5 1.0 PG A:MAP502 3.2 25.0 1.0 O3A A:MAP502 3.2 29.7 1.0 CD A:GLU305 3.3 26.4 1.0 N3B A:MAP502 3.3 30.9 1.0 OE1 A:GLU305 3.7 29.9 1.0 O A:HOH719 3.9 23.6 1.0 C5' A:MAP502 3.9 34.6 1.0 O5' A:MAP502 4.0 33.9 1.0 O3G A:MAP502 4.0 30.0 1.0 O A:HOH716 4.0 26.0 1.0 O1A A:MAP502 4.2 33.3 1.0 O2B A:MAP502 4.3 35.1 1.0 O1G A:MAP502 4.3 26.6 1.0 NZ A:LYS303 4.3 23.8 1.0 O A:HOH784 4.3 34.8 1.0 CG A:GLU305 4.5 24.6 1.0 O3' A:MAP502 4.7 35.4 1.0 CE A:LYS303 4.7 21.8 1.0 C3' A:MAP502 4.8 33.4 1.0 C4' A:MAP502 5.0 33.2 1.0

M.N.Lisa, A.Sogues, N.Barilone, M.Baumgart, M.Gil, M.Grana, R.Duran, R.M.Biondi, M.Bellinzoni, M.Bott, P.M.Alzari. A Tetratricopeptide Repeat Scaffold Couples Signal Detection to Odhi Phosphorylation in Metabolic Control By the Protein Kinase Pkng Mbio 2021.
ISSN: ESSN 2150-7511
PubMed: 34607462
DOI: 10.1128/MBIO.01717-21