Magnesium in PDB 7mxk: Crystal Structure of the S/T Protein Kinase Pkng From Corynebacterium Glutamicum (Residues 130-433) in Complex with Amp-Pnp, Isoform 2

The structure of Crystal Structure of the S/T Protein Kinase Pkng From Corynebacterium Glutamicum (Residues 130-433) in Complex with Amp-Pnp, Isoform 2, PDB code: 7mxk was solved by M.N.Lisa, P.M.Alzari, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.77 / 1.99
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	37.807, 54.594, 146.492, 90, 90, 90
R / Rfree (%)	20.9 / 23.7

The binding sites of Magnesium atom in the Crystal Structure of the S/T Protein Kinase Pkng From Corynebacterium Glutamicum (Residues 130-433) in Complex with Amp-Pnp, Isoform 2 (pdb code 7mxk). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the S/T Protein Kinase Pkng From Corynebacterium Glutamicum (Residues 130-433) in Complex with Amp-Pnp, Isoform 2, PDB code: 7mxk:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the S/T Protein Kinase Pkng From Corynebacterium Glutamicum (Residues 130-433) in Complex with Amp-Pnp, Isoform 2


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the S/T Protein Kinase Pkng From Corynebacterium Glutamicum (Residues 130-433) in Complex with Amp-Pnp, Isoform 2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg501 b:21.9 occ:1.00 OD2 A:ASP301 1.9 20.2 1.0 OD2 A:ASP318 2.0 22.4 1.0 O A:HOH628 2.0 27.5 1.0 O3G A:MAP502 2.0 22.6 1.0 O A:HOH615 2.0 21.5 1.0 O A:HOH640 2.2 20.0 1.0 CG A:ASP318 3.0 25.2 1.0 CG A:ASP301 3.1 22.1 1.0 PG A:MAP502 3.3 25.8 1.0 OD1 A:ASP318 3.5 28.1 1.0 O2G A:MAP502 3.7 24.1 1.0 O A:HOH624 3.8 29.4 1.0 CB A:ASP301 3.8 19.1 1.0 N3B A:MAP502 3.9 27.4 1.0 OD1 A:ASP301 4.1 19.8 1.0 ND2 A:ASN306 4.2 21.2 1.0 O1A A:MAP502 4.3 32.2 1.0 CB A:ASP318 4.3 23.4 1.0 O A:TYR332 4.3 26.6 1.0 O1G A:MAP502 4.5 29.8 1.0 CE A:LYS303 4.5 24.5 1.0 NZ A:LYS303 4.7 25.3 1.0 O A:HOH740 4.7 36.7 1.0 CB A:ALA321 4.7 25.9 1.0 NZ A:LYS205 4.9 32.8 1.0 O A:ASP301 5.0 20.9 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the S/T Protein Kinase Pkng From Corynebacterium Glutamicum (Residues 130-433) in Complex with Amp-Pnp, Isoform 2


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the S/T Protein Kinase Pkng From Corynebacterium Glutamicum (Residues 130-433) in Complex with Amp-Pnp, Isoform 2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg502 b:29.2 occ:1.00 MG A:MAP502 0.0 29.2 1.0 O1B A:MAP502 1.9 28.9 1.0 O2A A:MAP502 2.0 25.4 1.0 O2G A:MAP502 2.0 24.1 1.0 OE2 A:GLU305 2.1 30.7 1.0 O A:HOH688 2.2 31.3 1.0 O A:HOH672 2.2 31.7 1.0 PB A:MAP502 2.9 30.0 1.0 PA A:MAP502 3.1 30.1 1.0 PG A:MAP502 3.2 25.8 1.0 CD A:GLU305 3.2 33.6 1.0 O3A A:MAP502 3.3 28.8 1.0 N3B A:MAP502 3.4 27.4 1.0 O1G A:MAP502 3.8 29.8 1.0 O A:HOH640 3.9 20.0 1.0 OE1 A:GLU305 4.0 41.4 1.0 NZ A:LYS303 4.0 25.3 1.0 O5' A:MAP502 4.1 33.9 1.0 C5' A:MAP502 4.1 35.1 1.0 CG A:GLU305 4.1 30.9 1.0 O1A A:MAP502 4.2 32.2 1.0 O2B A:MAP502 4.3 37.9 1.0 CE A:LYS303 4.4 24.5 1.0 O3G A:MAP502 4.5 22.6 1.0 N A:GLY185 4.5 37.1 1.0 O A:HOH695 4.6 38.6 1.0 CA A:GLY184 4.8 39.3 1.0 O A:HOH664 4.9 29.3 1.0

M.N.Lisa, A.Sogues, N.Barilone, M.Baumgart, M.Gil, M.Grana, R.Duran, R.M.Biondi, M.Bellinzoni, M.Bott, P.M.Alzari. A Tetratricopeptide Repeat Scaffold Couples Signal Detection to Odhi Phosphorylation in Metabolic Control By the Protein Kinase Pkng Mbio 2021.
ISSN: ESSN 2150-7511
PubMed: 34607462
DOI: 10.1128/MBIO.01717-21