Magnesium in PDB 7obl: Crystal Structure of 14-3-3 Sigma in Complex with Pkr Phosphopeptide and Stabilizer Fusicoccin-A

All present enzymatic activity of Crystal Structure of 14-3-3 Sigma in Complex with Pkr Phosphopeptide and Stabilizer Fusicoccin-A:
2.7.10.2; 2.7.11.1;

The structure of Crystal Structure of 14-3-3 Sigma in Complex with Pkr Phosphopeptide and Stabilizer Fusicoccin-A, PDB code: 7obl was solved by F.Centorrino, B.Andlovic, C.Ottmann, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	34.48 / 1.80
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	82.625, 112.306, 62.616, 90, 90, 90
R / Rfree (%)	21.8 / 23.9

The binding sites of Magnesium atom in the Crystal Structure of 14-3-3 Sigma in Complex with Pkr Phosphopeptide and Stabilizer Fusicoccin-A (pdb code 7obl). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of 14-3-3 Sigma in Complex with Pkr Phosphopeptide and Stabilizer Fusicoccin-A, PDB code: 7obl:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of 14-3-3 Sigma in Complex with Pkr Phosphopeptide and Stabilizer Fusicoccin-A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of 14-3-3 Sigma in Complex with Pkr Phosphopeptide and Stabilizer Fusicoccin-A within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:6.5 occ:0.50 O A:HOH536 2.2 17.4 1.0 OE1 A:GLU2 2.5 8.6 1.0 O A:HOH430 2.6 8.8 1.0 O A:HOH711 2.6 19.4 1.0 CD A:GLU2 3.5 8.2 1.0 OE2 A:GLU2 3.8 6.8 1.0 O A:HOH672 4.0 18.5 1.0 HA A:GLU2 4.0 4.2 1.0 H A:ARG3 4.2 6.9 1.0 O A:HOH588 4.4 8.9 1.0 O A:HOH528 4.6 4.6 1.0 HB3 A:GLU2 4.7 4.8 1.0 CG A:GLU2 4.7 3.1 1.0 CA A:GLU2 4.9 3.5 1.0 HG2 A:GLU2 4.9 3.7 1.0 N A:ARG3 5.0 5.7 1.0 CB A:GLU2 5.0 4.0 1.0 O A:HOH526 5.0 7.3 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of 14-3-3 Sigma in Complex with Pkr Phosphopeptide and Stabilizer Fusicoccin-A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of 14-3-3 Sigma in Complex with Pkr Phosphopeptide and Stabilizer Fusicoccin-A within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg303 b:10.2 occ:1.00 O A:HOH468 2.2 21.3 1.0 OE2 A:GLU35 2.3 19.0 1.0 O A:GLU110 2.3 14.4 1.0 O A:HOH654 2.7 27.7 1.0 CD A:GLU35 2.9 24.8 1.0 OE1 A:GLU35 2.9 25.4 1.0 H A:GLY112 3.5 21.4 1.0 C A:GLU110 3.5 12.9 1.0 HB3 A:GLU110 3.8 23.7 1.0 HA A:ALA111 3.9 19.0 1.0 N A:GLY112 4.1 17.9 1.0 HA A:GLU110 4.3 23.0 1.0 CG A:GLU35 4.3 18.1 1.0 O A:HOH427 4.4 21.8 1.0 CA A:GLU110 4.4 19.1 1.0 N A:ALA111 4.4 14.4 1.0 HG2 A:GLU35 4.5 21.7 1.0 CA A:ALA111 4.5 15.8 1.0 HA3 A:GLY112 4.5 20.8 1.0 CB A:GLU110 4.5 19.8 1.0 O A:HOH594 4.6 17.9 1.0 OE2 A:GLU110 4.7 21.4 1.0 C A:ALA111 4.7 15.7 1.0 HG3 A:GLU35 4.7 21.7 1.0 CA A:GLY112 4.9 17.4 1.0 HB2 A:GLU110 5.0 23.7 1.0

B.Andlovic, G.Heilmann, S.Ninck, S.Andrei, F.Centorrino, Y.Higuchi, N.Kato, L.Brunsveld, S.Menninger, A.Choidas, A.Wolf, M.Kaiser, J.Eickhoff, C.Ottmann. Inf Alpha Primes Ovarian Cancer Cells For Fusicoccin-Induced Cell Death Via Stabilization of 14-3-3 Protein-Protein Interactions To Be Published.