Magnesium in PDB 7oge: Gtpase Hras Mutant D33K Under 200 Mpa Pressure

All present enzymatic activity of Gtpase Hras Mutant D33K Under 200 Mpa Pressure:
3.6.5.2;

The structure of Gtpase Hras Mutant D33K Under 200 Mpa Pressure, PDB code: 7oge was solved by N.C.Colloc'h, E.Girard, T.Prange, H.R.Kalbitzer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.10
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	39.568, 39.568, 160.645, 90, 90, 120
R / Rfree (%)	17.3 / 26.3

The binding sites of Magnesium atom in the Gtpase Hras Mutant D33K Under 200 Mpa Pressure (pdb code 7oge). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Gtpase Hras Mutant D33K Under 200 Mpa Pressure, PDB code: 7oge:

Magnesium binding site 1 out of 1 in the Gtpase Hras Mutant D33K Under 200 Mpa Pressure


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Gtpase Hras Mutant D33K Under 200 Mpa Pressure within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg202 b:21.3 occ:1.00 O2G A:GNP201 1.9 18.4 1.0 O2B A:GNP201 2.0 16.7 1.0 O A:HOH302 2.0 15.7 1.0 OG A:SER17 2.2 15.7 1.0 OG1 A:THR35 2.2 23.2 1.0 O A:HOH301 2.2 15.1 1.0 CB A:THR35 3.1 23.4 1.0 PB A:GNP201 3.1 17.7 1.0 CB A:SER17 3.2 16.2 1.0 PG A:GNP201 3.2 18.7 1.0 N3B A:GNP201 3.3 18.4 1.0 N A:THR35 3.8 22.3 1.0 N A:SER17 3.9 15.6 1.0 OD2 A:ASP57 4.1 26.2 1.0 O2A A:GNP201 4.1 18.6 1.0 CA A:SER17 4.1 15.9 1.0 CA A:THR35 4.1 23.8 1.0 O1B A:GNP201 4.1 16.5 1.0 O3A A:GNP201 4.2 17.7 1.0 O1G A:GNP201 4.2 20.2 1.0 CG2 A:THR35 4.2 24.6 1.0 O3G A:GNP201 4.2 17.4 1.0 OD1 A:ASP57 4.4 25.4 1.0 O A:LYS33 4.4 20.2 1.0 O A:THR58 4.5 25.2 1.0 PA A:GNP201 4.5 16.2 1.0 CG A:ASP57 4.6 23.2 1.0 O A:HOH351 4.6 30.6 1.0 O1A A:GNP201 4.7 21.0 1.0 C A:PRO34 4.7 23.2 1.0 CB A:LYS16 4.8 15.2 1.0 CE A:LYS16 4.8 14.6 1.0 CA A:PRO34 5.0 24.1 1.0 C A:LYS16 5.0 15.9 1.0 NZ A:LYS16 5.0 15.4 1.0

E.Girard, P.Lopes, M.Spoerner, A.C.Dhaussy, T.Prange, H.R.Kalbitzer, N.Colloc'h. Equilibria Between Conformational States of the Ras Oncogene Protein Revealed By High Pressure Crystallography. Chem Sci V. 13 2001 2022.
ISSN: ISSN 2041-6520
PubMed: 35308861
DOI: 10.1039/D1SC05488K