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Magnesium in PDB 7owf: Klentaq Dna Polymerase in A Ternary Complex with Primer/Template and the Fluorescent Nucleotide Analog Bfdutp
Enzymatic activity of Klentaq Dna Polymerase in A Ternary Complex with Primer/Template and the Fluorescent Nucleotide Analog Bfdutp
All present enzymatic activity of Klentaq Dna Polymerase in A Ternary Complex with Primer/Template and the Fluorescent Nucleotide Analog Bfdutp:
2.7.7.7;
2.7.7.7;
Protein crystallography data
The structure of Klentaq Dna Polymerase in A Ternary Complex with Primer/Template and the Fluorescent Nucleotide Analog Bfdutp, PDB code: 7owf
was solved by
K.Betz,
S.G.Srivatsan,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
| Resolution Low / High (Å) | 47.71 / 1.91 |
| Space group | P 31 2 1 |
| Cell size a, b, c (Å), α, β, γ (°) | 110.175, 110.175, 91.296, 90, 90, 120 |
| R / Rfree (%) | 19.5 / 24.1 |
Magnesium Binding Sites:
The binding sites of Magnesium atom in the Klentaq Dna Polymerase in A Ternary Complex with Primer/Template and the Fluorescent Nucleotide Analog Bfdutp
(pdb code 7owf). This binding sites where shown within
5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Klentaq Dna Polymerase in A Ternary Complex with Primer/Template and the Fluorescent Nucleotide Analog Bfdutp, PDB code: 7owf:
Jump to Magnesium binding site number: 1; 2; 3;
In total 3 binding sites of Magnesium where determined in the Klentaq Dna Polymerase in A Ternary Complex with Primer/Template and the Fluorescent Nucleotide Analog Bfdutp, PDB code: 7owf:
Jump to Magnesium binding site number: 1; 2; 3;
Magnesium binding site 1 out of 3 in 7owf
Go back to
Magnesium Binding Sites List in 7owf
Magnesium binding site 1 out
of 3 in the Klentaq Dna Polymerase in A Ternary Complex with Primer/Template and the Fluorescent Nucleotide Analog Bfdutp
Mono view
Stereo pair view
Mono view
Stereo pair view
A full contact list of Magnesium with other atoms in the Mg binding
site number 1 of Klentaq Dna Polymerase in A Ternary Complex with Primer/Template and the Fluorescent Nucleotide Analog Bfdutp within 5.0Å range:
|
Magnesium binding site 2 out of 3 in 7owf
Go back to
Magnesium Binding Sites List in 7owf
Magnesium binding site 2 out
of 3 in the Klentaq Dna Polymerase in A Ternary Complex with Primer/Template and the Fluorescent Nucleotide Analog Bfdutp
Mono view
Stereo pair view
Mono view
Stereo pair view
A full contact list of Magnesium with other atoms in the Mg binding
site number 2 of Klentaq Dna Polymerase in A Ternary Complex with Primer/Template and the Fluorescent Nucleotide Analog Bfdutp within 5.0Å range:
|
Magnesium binding site 3 out of 3 in 7owf
Go back to
Magnesium Binding Sites List in 7owf
Magnesium binding site 3 out
of 3 in the Klentaq Dna Polymerase in A Ternary Complex with Primer/Template and the Fluorescent Nucleotide Analog Bfdutp
Mono view
Stereo pair view
Mono view
Stereo pair view
A full contact list of Magnesium with other atoms in the Mg binding
site number 3 of Klentaq Dna Polymerase in A Ternary Complex with Primer/Template and the Fluorescent Nucleotide Analog Bfdutp within 5.0Å range:
|
Reference:
P.Ghosh,
H.M.Kropp,
K.Betz,
S.Ludmann,
K.Diederichs,
A.Marx,
S.G.Srivatsan.
Microenvironment-Sensitive Fluorescent Nucleotide Probes From Benzofuran, Benzothiophene, and Selenophene As Substrates For Dna Polymerases. J.Am.Chem.Soc. V. 144 10556 2022.
ISSN: ESSN 1520-5126
PubMed: 35666775
DOI: 10.1021/JACS.2C03454
Page generated: Thu Apr 6 18:27:29 2023
ISSN: ESSN 1520-5126
PubMed: 35666775
DOI: 10.1021/JACS.2C03454
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