Magnesium in PDB 7p17: F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 12KEV)

The structure of F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 12KEV), PDB code: 7p17 was solved by A.G.Gabdulkhakov, G.K.Selikhanov, S.Guenther, A.Meents, T.Y.Fufina, L.G.Vasilieva, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.05 / 2.22
Space group	P 42 21 2
Cell size a, b, c (Å), α, β, γ (°)	102.5, 102.5, 237.4, 90, 90, 90
R / Rfree (%)	17.7 / 21.5

The structure of F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 12KEV) also contains other interesting chemical elements:

Iron

(Fe)

1 atom

The binding sites of Magnesium atom in the F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 12KEV) (pdb code 7p17). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 12KEV), PDB code: 7p17:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 12KEV)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 12KEV) within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg504 b:34.5 occ:1.00 MG L:BCL504 0.0 34.5 1.0 ND L:BCL504 2.0 40.4 1.0 NB L:BCL504 2.1 38.2 1.0 NE2 L:HIS173 2.2 32.4 1.0 NC L:BCL504 2.2 38.4 1.0 NA L:BCL504 2.2 32.8 1.0 CE1 L:HIS173 2.9 35.4 1.0 C4D L:BCL504 3.0 39.1 1.0 C4B L:BCL504 3.1 37.0 1.0 C1B L:BCL504 3.1 35.0 1.0 C1C L:BCL504 3.2 43.0 1.0 C4A L:BCL504 3.2 36.6 1.0 C1D L:BCL504 3.2 38.5 1.0 C4C L:BCL504 3.2 39.0 1.0 C1A L:BCL504 3.2 41.7 1.0 CD2 L:HIS173 3.3 36.0 1.0 CHC L:BCL504 3.4 39.3 1.0 CHB L:BCL504 3.4 40.0 1.0 CBB M:BCL406 3.5 36.9 1.0 CHA L:BCL504 3.5 38.9 1.0 CHD L:BCL504 3.5 39.2 1.0 ND1 L:HIS173 4.1 34.3 1.0 CAB M:BCL406 4.2 40.9 1.0 C3D L:BCL504 4.3 40.8 1.0 C2B L:BCL504 4.3 40.2 1.0 CG L:HIS173 4.3 36.8 1.0 C2D L:BCL504 4.3 42.0 1.0 C3B L:BCL504 4.4 40.5 1.0 C3A L:BCL504 4.5 41.1 1.0 C2C L:BCL504 4.5 40.5 1.0 C3C L:BCL504 4.5 37.8 1.0 C2A L:BCL504 4.6 36.1 1.0 CD2 L:PHE167 4.7 49.5 1.0 C3B M:BCL406 4.8 37.7 1.0 OBB M:BCL406 4.8 36.6 1.0 CBD L:BCL504 5.0 43.2 1.0 CMA L:BCL504 5.0 33.2 1.0

Magnesium binding site 2 out of 4 in the F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 12KEV)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 12KEV) within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg505 b:37.5 occ:1.00 MG L:BCL505 0.0 37.5 1.0 ND L:BCL505 2.0 33.9 1.0 NB L:BCL505 2.1 29.9 1.0 NC L:BCL505 2.1 30.0 1.0 NA L:BCL505 2.2 31.1 1.0 NE2 L:HIS153 2.3 44.1 1.0 C4D L:BCL505 3.0 34.6 1.0 C1C L:BCL505 3.1 33.3 1.0 CD2 L:HIS153 3.1 41.8 1.0 C4B L:BCL505 3.1 33.7 1.0 C1B L:BCL505 3.1 31.0 1.0 C1D L:BCL505 3.1 34.3 1.0 C4A L:BCL505 3.2 37.1 1.0 C4C L:BCL505 3.2 34.3 1.0 C1A L:BCL505 3.2 32.0 1.0 CE1 L:HIS153 3.4 38.9 1.0 CHC L:BCL505 3.4 32.3 1.0 CHB L:BCL505 3.4 33.9 1.0 CHA L:BCL505 3.4 35.1 1.0 CHD L:BCL505 3.5 32.7 1.0 C3D L:BCL505 4.2 35.9 1.0 C2D L:BCL505 4.3 31.2 1.0 C2B L:BCL505 4.3 31.7 1.0 CG L:HIS153 4.3 40.2 1.0 C3B L:BCL505 4.3 32.8 1.0 CBB L:BPH507 4.4 34.6 1.0 C2C L:BCL505 4.4 35.3 1.0 ND1 L:HIS153 4.4 44.5 1.0 C3C L:BCL505 4.5 30.8 1.0 C3A L:BCL505 4.5 32.7 1.0 C2A L:BCL505 4.5 34.6 1.0 CE2 M:TYR210 4.8 32.2 1.0 CD1 L:LEU154 4.9 41.6 1.0 CBD L:BCL505 4.9 38.1 1.0

Magnesium binding site 3 out of 4 in the F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 12KEV)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 12KEV) within 5.0Å range: probe atom residue distance (Å) B Occ M:Mg405 b:38.6 occ:1.00 MG M:BCL405 0.0 38.6 1.0 ND M:BCL405 2.0 35.6 1.0 NB M:BCL405 2.1 33.7 1.0 NC M:BCL405 2.1 28.2 1.0 NA M:BCL405 2.2 40.4 1.0 NE2 M:HIS182 2.3 41.7 1.0 C4D M:BCL405 3.0 38.8 1.0 C1B M:BCL405 3.1 35.2 1.0 C4B M:BCL405 3.1 34.4 1.0 CD2 M:HIS182 3.1 35.7 1.0 C1C M:BCL405 3.1 38.0 1.0 C4A M:BCL405 3.1 38.9 1.0 C1D M:BCL405 3.1 29.8 1.0 C4C M:BCL405 3.2 39.0 1.0 C1A M:BCL405 3.2 35.4 1.0 CE1 M:HIS182 3.4 38.2 1.0 CHC M:BCL405 3.4 31.3 1.0 CHB M:BCL405 3.4 37.8 1.0 CHA M:BCL405 3.5 34.2 1.0 CHD M:BCL405 3.5 35.7 1.0 C3D M:BCL405 4.2 38.5 1.0 C2B M:BCL405 4.3 38.6 1.0 C2D M:BCL405 4.3 38.1 1.0 CG M:HIS182 4.3 40.9 1.0 C3B M:BCL405 4.4 40.0 1.0 ND1 M:HIS182 4.4 42.2 1.0 C3A M:BCL405 4.4 42.8 1.0 C2C M:BCL405 4.4 37.8 1.0 C3C M:BCL405 4.5 34.0 1.0 C2A M:BCL405 4.5 44.6 1.0 CBB L:BPH506 4.6 40.6 1.0 CBD M:BCL405 4.9 39.9 1.0 CM5 M:SPN404 4.9 53.1 1.0 CBA M:BCL405 5.0 39.3 1.0

Magnesium binding site 4 out of 4 in the F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 12KEV)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 12KEV) within 5.0Å range: probe atom residue distance (Å) B Occ M:Mg406 b:36.6 occ:1.00 MG M:BCL406 0.0 36.6 1.0 ND M:BCL406 2.0 38.8 1.0 NB M:BCL406 2.1 39.4 1.0 NC M:BCL406 2.1 41.2 1.0 NA M:BCL406 2.2 33.2 1.0 NE2 M:HIS202 2.2 37.0 1.0 C4D M:BCL406 3.0 37.6 1.0 C4B M:BCL406 3.1 39.0 1.0 CE1 M:HIS202 3.1 36.9 1.0 C1B M:BCL406 3.1 37.0 1.0 C1C M:BCL406 3.1 37.3 1.0 C1D M:BCL406 3.1 44.3 1.0 C4A M:BCL406 3.2 36.7 1.0 C4C M:BCL406 3.2 39.0 1.0 C1A M:BCL406 3.2 38.2 1.0 CD2 M:HIS202 3.3 31.6 1.0 CHC M:BCL406 3.4 36.1 1.0 CHB M:BCL406 3.4 33.7 1.0 CHA M:BCL406 3.4 39.5 1.0 CBB L:BCL504 3.5 40.3 1.0 CHD M:BCL406 3.5 35.1 1.0 CAB L:BCL504 3.9 42.7 1.0 OBB L:BCL504 4.1 34.7 1.0 C3D M:BCL406 4.2 39.9 1.0 ND1 M:HIS202 4.3 34.0 1.0 C2D M:BCL406 4.3 37.8 1.0 C2B M:BCL406 4.3 35.9 1.0 C3B M:BCL406 4.3 37.7 1.0 C2C M:BCL406 4.4 36.9 1.0 CG M:HIS202 4.4 36.6 1.0 C3C M:BCL406 4.5 37.8 1.0 C3A M:BCL406 4.5 34.8 1.0 C2A M:BCL406 4.5 37.0 1.0 C3B L:BCL504 4.6 40.5 1.0 CBD M:BCL406 4.9 41.5 1.0 CMC M:BCL406 4.9 35.8 1.0

A.G.Gabdulkhakov, G.K.Selikhanov, S.Guenther, A.Meents, T.Y.Fufina, L.G.Vasilieva. X-Ray Structure of Rhodobacter Sphaeroides Reaction Center with M197 Phe-His Substitution Clarifies Properties of the Mutant Complex To Be Published.