Magnesium in PDB 7p2c: F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 26KEV)

The structure of F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 26KEV), PDB code: 7p2c was solved by A.G.Gabdulkhakov, G.K.Selikhanov, S.Guenther, A.Meents, T.Y.Fufina, L.G.Vasilieva, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.05 / 2.04
Space group	P 42 21 2
Cell size a, b, c (Å), α, β, γ (°)	102.5, 102.5, 237.4, 90, 90, 90
R / Rfree (%)	20.1 / 22.9

The structure of F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 26KEV) also contains other interesting chemical elements:

Iron

(Fe)

1 atom

The binding sites of Magnesium atom in the F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 26KEV) (pdb code 7p2c). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 26KEV), PDB code: 7p2c:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 26KEV)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 26KEV) within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg304 b:29.1 occ:1.00 MG L:BCL304 0.0 29.1 1.0 ND L:BCL304 2.0 41.9 1.0 NE2 L:HIS173 2.1 30.7 1.0 NB L:BCL304 2.1 35.3 1.0 NC L:BCL304 2.2 42.4 1.0 NA L:BCL304 2.2 28.9 1.0 CE1 L:HIS173 2.8 33.4 1.0 C4D L:BCL304 3.0 40.9 1.0 C1B L:BCL304 3.1 33.4 1.0 C4B L:BCL304 3.1 37.9 1.0 C1C L:BCL304 3.2 45.0 1.0 C1D L:BCL304 3.2 43.1 1.0 C4A L:BCL304 3.2 36.1 1.0 C4C L:BCL304 3.2 40.5 1.0 C1A L:BCL304 3.3 38.4 1.0 CD2 L:HIS173 3.3 26.9 1.0 CHB L:BCL304 3.4 27.5 1.0 CHC L:BCL304 3.4 40.8 1.0 CBB M:BCL405 3.4 33.4 1.0 CHD L:BCL304 3.5 41.0 1.0 CHA L:BCL304 3.5 39.9 1.0 ND1 L:HIS173 4.0 31.7 1.0 CAB M:BCL405 4.2 32.6 1.0 CG L:HIS173 4.3 36.5 1.0 C3D L:BCL304 4.3 43.2 1.0 C2B L:BCL304 4.3 36.3 1.0 C2D L:BCL304 4.3 43.5 1.0 C3B L:BCL304 4.4 38.2 1.0 C2C L:BCL304 4.5 41.7 1.0 C3A L:BCL304 4.5 31.7 1.0 C3C L:BCL304 4.5 35.3 1.0 C2A L:BCL304 4.6 29.3 1.0 C3B M:BCL405 4.7 33.5 1.0 OBB M:BCL405 4.9 37.9 1.0 CD2 L:PHE167 4.9 43.2 1.0

Magnesium binding site 2 out of 4 in the F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 26KEV)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 26KEV) within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg305 b:34.3 occ:1.00 MG L:BCL305 0.0 34.3 1.0 ND L:BCL305 2.0 30.1 1.0 NB L:BCL305 2.1 33.7 1.0 NC L:BCL305 2.1 27.6 1.0 NE2 L:HIS153 2.2 43.7 1.0 NA L:BCL305 2.2 37.3 1.0 C4D L:BCL305 3.0 30.5 1.0 C4B L:BCL305 3.1 31.6 1.0 C1B L:BCL305 3.1 34.8 1.0 C1C L:BCL305 3.1 30.1 1.0 CE1 L:HIS153 3.1 43.8 1.0 CD2 L:HIS153 3.1 44.4 1.0 C1D L:BCL305 3.2 35.8 1.0 C4A L:BCL305 3.2 33.1 1.0 C4C L:BCL305 3.2 33.1 1.0 C1A L:BCL305 3.2 31.0 1.0 CHC L:BCL305 3.4 33.2 1.0 CHB L:BCL305 3.4 38.0 1.0 CHA L:BCL305 3.5 34.7 1.0 CHD L:BCL305 3.5 32.1 1.0 C3D L:BCL305 4.2 37.2 1.0 ND1 L:HIS153 4.2 36.7 1.0 CG L:HIS153 4.3 43.1 1.0 C2B L:BCL305 4.3 31.3 1.0 C2D L:BCL305 4.3 29.3 1.0 C3B L:BCL305 4.3 33.0 1.0 C2C L:BCL305 4.4 36.3 1.0 C3C L:BCL305 4.5 32.4 1.0 C3A L:BCL305 4.5 33.0 1.0 CBB L:BPH306 4.5 32.1 1.0 C2A L:BCL305 4.5 35.0 1.0 CD1 L:LEU154 4.9 37.1 1.0 CE1 M:TYR210 4.9 27.1 1.0 CBD L:BCL305 4.9 38.1 1.0 C19 L:BPH306 5.0 38.6 1.0 CBC L:BCL305 5.0 35.9 1.0

Magnesium binding site 3 out of 4 in the F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 26KEV)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 26KEV) within 5.0Å range: probe atom residue distance (Å) B Occ M:Mg404 b:33.5 occ:1.00 MG M:BCL404 0.0 33.5 1.0 ND M:BCL404 2.0 37.4 1.0 NB M:BCL404 2.1 33.3 1.0 NC M:BCL404 2.1 33.4 1.0 NA M:BCL404 2.2 29.0 1.0 NE2 M:HIS182 2.3 38.9 1.0 C4D M:BCL404 3.0 34.4 1.0 C1B M:BCL404 3.1 33.8 1.0 C4B M:BCL404 3.1 33.0 1.0 C1D M:BCL404 3.1 35.4 1.0 C1C M:BCL404 3.1 40.9 1.0 C4A M:BCL404 3.1 37.5 1.0 C4C M:BCL404 3.2 35.1 1.0 C1A M:BCL404 3.2 30.5 1.0 CE1 M:HIS182 3.3 41.1 1.0 CD2 M:HIS182 3.3 40.4 1.0 CHB M:BCL404 3.4 36.1 1.0 CHC M:BCL404 3.4 32.5 1.0 CHA M:BCL404 3.5 34.3 1.0 CHD M:BCL404 3.5 36.0 1.0 C3D M:BCL404 4.2 36.6 1.0 C2D M:BCL404 4.3 36.4 1.0 C2B M:BCL404 4.3 33.7 1.0 C3B M:BCL404 4.3 34.4 1.0 ND1 M:HIS182 4.4 36.9 1.0 CG M:HIS182 4.4 40.0 1.0 C3A M:BCL404 4.4 37.8 1.0 C2C M:BCL404 4.4 37.5 1.0 C3C M:BCL404 4.5 34.5 1.0 C2A M:BCL404 4.5 36.4 1.0 CBB M:BPH406 4.6 34.6 1.0 CBD M:BCL404 4.9 39.2 1.0

Magnesium binding site 4 out of 4 in the F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 26KEV)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of F(M197)H Mutant Structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides Strain Rv By Fixed-Target Serial Synchrotron Crystallography (Room Temperature, 26KEV) within 5.0Å range: probe atom residue distance (Å) B Occ M:Mg405 b:31.4 occ:1.00 MG M:BCL405 0.0 31.4 1.0 ND M:BCL405 2.0 31.8 1.0 NB M:BCL405 2.1 31.9 1.0 NC M:BCL405 2.1 35.8 1.0 NA M:BCL405 2.2 35.8 1.0 NE2 M:HIS202 2.2 26.7 1.0 C4D M:BCL405 3.0 33.8 1.0 C4B M:BCL405 3.1 33.9 1.0 C1B M:BCL405 3.1 31.4 1.0 C1C M:BCL405 3.1 32.2 1.0 C1D M:BCL405 3.2 39.6 1.0 C4A M:BCL405 3.2 31.3 1.0 C4C M:BCL405 3.2 31.8 1.0 CE1 M:HIS202 3.2 27.2 1.0 C1A M:BCL405 3.2 35.7 1.0 CD2 M:HIS202 3.3 26.8 1.0 CHC M:BCL405 3.4 31.8 1.0 CHB M:BCL405 3.4 29.3 1.0 CHA M:BCL405 3.4 31.8 1.0 CHD M:BCL405 3.5 29.2 1.0 CBB L:BCL304 3.9 34.7 1.0 CAB L:BCL304 4.0 38.1 1.0 OBB L:BCL304 4.2 34.2 1.0 C3D M:BCL405 4.2 36.5 1.0 C2B M:BCL405 4.3 27.9 1.0 ND1 M:HIS202 4.3 25.8 1.0 C2D M:BCL405 4.3 40.9 1.0 C3B M:BCL405 4.3 33.5 1.0 CG M:HIS202 4.4 29.4 1.0 C2C M:BCL405 4.4 30.6 1.0 C3C M:BCL405 4.5 30.7 1.0 C3A M:BCL405 4.5 30.9 1.0 C2A M:BCL405 4.5 28.6 1.0 C3B L:BCL304 4.6 38.2 1.0 CBD M:BCL405 4.9 30.3 1.0 CD1 M:ILE206 5.0 28.7 1.0

G.Selikhanov, T.Fufina, S.Guenther, A.Meents, A.Gabdulkhakov, L.Vasilieva. X-Ray Structure of the Rhodobacter Sphaeroides Reaction Center with An M197 Phe→His Substitution Clarifies the Properties of the Mutant Complex. Iucrj V. 9 261 2022.
ISSN: ESSN 2052-2525
PubMed: 35371503
DOI: 10.1107/S2052252521013178