Magnesium in PDB 7q1e: Cpap:Tubulin:IIH5 Alpharep Complex

The structure of Cpap:Tubulin:IIH5 Alpharep Complex, PDB code: 7q1e was solved by V.Campanacci, B.Gigant, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.84 / 2.70
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	53.59, 68.05, 420.92, 90, 90, 90
R / Rfree (%)	20 / 25.4

The binding sites of Magnesium atom in the Cpap:Tubulin:IIH5 Alpharep Complex (pdb code 7q1e). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Cpap:Tubulin:IIH5 Alpharep Complex, PDB code: 7q1e:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Cpap:Tubulin:IIH5 Alpharep Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cpap:Tubulin:IIH5 Alpharep Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg502 b:82.7 occ:1.00 O A:HOH605 1.9 72.2 1.0 O1B A:GTP501 1.9 87.9 1.0 O A:HOH610 1.9 84.2 1.0 O2G A:GTP501 2.1 89.7 1.0 O A:HOH611 2.1 101.9 1.0 O A:HOH602 2.3 75.3 1.0 PB A:GTP501 3.1 87.9 1.0 PG A:GTP501 3.3 88.9 1.0 O3B A:GTP501 3.5 88.4 1.0 O3A A:GTP501 3.6 87.7 1.0 O3G A:GTP501 3.8 88.7 1.0 OD2 A:ASP69 3.9 93.0 1.0 CB A:GLN11 3.9 80.2 1.0 N A:GLN11 4.1 77.1 1.0 OE2 A:GLU71 4.1 117.2 1.0 NZ B:LYS254 4.2 103.0 1.0 O1A A:GTP501 4.3 87.2 1.0 O2B A:GTP501 4.4 87.7 1.0 O1G A:GTP501 4.4 88.9 1.0 CG A:GLU71 4.4 106.3 1.0 PA A:GTP501 4.5 87.3 1.0 OD1 A:ASP69 4.5 93.9 1.0 CA A:GLN11 4.6 78.4 1.0 CG A:ASP69 4.7 93.5 1.0 CD A:GLU71 4.7 113.4 1.0 CB A:ASP98 4.7 103.0 1.0 OD2 A:ASP98 4.9 107.8 1.0 O2A A:GTP501 4.9 87.3 1.0 OE1 A:GLN11 4.9 94.4 1.0

Magnesium binding site 2 out of 2 in the Cpap:Tubulin:IIH5 Alpharep Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Cpap:Tubulin:IIH5 Alpharep Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg502 b:118.7 occ:1.00 O B:HOH604 1.9 132.5 1.0 O B:HOH601 2.0 116.4 1.0 O1B B:GTP501 2.2 138.3 1.0 CB B:GLN11 3.2 120.0 1.0 PB B:GTP501 3.5 138.0 1.0 O1G B:GTP501 3.6 138.9 1.0 N B:GLN11 3.7 117.7 1.0 O3A B:GTP501 3.7 136.9 1.0 O2G B:GTP501 3.8 138.8 1.0 OD2 B:ASP69 3.8 136.0 1.0 OE1 B:GLN11 4.0 131.3 1.0 CA B:GLN11 4.0 118.5 1.0 PG B:GTP501 4.0 138.9 1.0 O3B B:GTP501 4.1 138.4 1.0 O1A B:GTP501 4.3 135.4 1.0 OD1 B:ASP69 4.3 134.0 1.0 CG B:GLN11 4.3 123.0 1.0 CD B:GLN11 4.4 128.4 1.0 CG B:ASP69 4.5 132.9 1.0 CB B:GLU71 4.5 141.6 1.0 PA B:GTP501 4.5 135.5 1.0 O2B B:GTP501 4.7 137.7 1.0 C B:GLY10 4.8 117.7 1.0 OG1 B:THR74 4.8 153.1 1.0 OE1 B:GLU71 4.9 160.3 1.0 O2A B:GTP501 4.9 135.3 1.0

V.Campanacci, A.Urvoas, L.Ammar Khodja, M.Aumont-Nicaise, M.Noiray, S.Lachkar, P.A.Curmi, P.Minard, B.Gigant. Structural Convergence For Tubulin Binding of Cpap and Vinca Domain Microtubule Inhibitors. Proc.Natl.Acad.Sci.Usa V. 119 98119 2022.
ISSN: ESSN 1091-6490
PubMed: 35507869
DOI: 10.1073/PNAS.2120098119