Atomistry » Magnesium » PDB 7rsc-7s61 » 7s4x
Atomistry »
  Magnesium »
    PDB 7rsc-7s61 »
      7s4x »

Magnesium in PDB 7s4x: CAS9:Grna in Complex with 18-20MM Dna, 1 Minute Time-Point, Kinked Active Conformation

Magnesium Binding Sites:

The binding sites of Magnesium atom in the CAS9:Grna in Complex with 18-20MM Dna, 1 Minute Time-Point, Kinked Active Conformation (pdb code 7s4x). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 5 binding sites of Magnesium where determined in the CAS9:Grna in Complex with 18-20MM Dna, 1 Minute Time-Point, Kinked Active Conformation, PDB code: 7s4x:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5;

Magnesium binding site 1 out of 5 in 7s4x

Go back to Magnesium Binding Sites List in 7s4x
Magnesium binding site 1 out of 5 in the CAS9:Grna in Complex with 18-20MM Dna, 1 Minute Time-Point, Kinked Active Conformation


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of CAS9:Grna in Complex with 18-20MM Dna, 1 Minute Time-Point, Kinked Active Conformation within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg1401

b:42.7
occ:1.00
OE1 A:GLU762 1.9 35.0 1.0
OD1 A:ASP10 2.0 30.0 1.0
CG A:ASP10 3.1 30.0 1.0
CD A:GLU762 3.1 35.0 1.0
P D:DC34 3.3 44.5 1.0
OD2 A:ASP10 3.6 30.0 1.0
OP1 D:DC34 3.8 44.5 1.0
OE2 A:GLU762 3.8 35.0 1.0
O5' D:DC34 4.0 44.5 1.0
C5' D:DC34 4.0 44.5 1.0
O A:ILE11 4.1 29.9 1.0
CG A:GLU762 4.1 35.0 1.0
MG A:MG1402 4.2 32.8 1.0
CB A:ASP10 4.3 30.0 1.0
OP2 D:DC34 4.3 44.5 1.0
CB A:GLU762 4.4 35.0 1.0
CA A:ASP10 4.5 30.0 1.0
C A:ASP10 4.6 30.0 1.0
N A:ILE11 4.6 29.9 1.0
C A:ILE11 4.9 29.9 1.0
OD2 A:ASP986 4.9 29.2 1.0
OG A:SER960 4.9 38.6 1.0

Magnesium binding site 2 out of 5 in 7s4x

Go back to Magnesium Binding Sites List in 7s4x
Magnesium binding site 2 out of 5 in the CAS9:Grna in Complex with 18-20MM Dna, 1 Minute Time-Point, Kinked Active Conformation


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of CAS9:Grna in Complex with 18-20MM Dna, 1 Minute Time-Point, Kinked Active Conformation within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg1402

b:32.8
occ:1.00
OP1 D:DC34 1.9 44.5 1.0
OD2 A:ASP10 2.0 30.0 1.0
ND1 A:HIS983 2.1 30.4 1.0
CE1 A:HIS983 2.8 30.4 1.0
CG A:ASP10 3.1 30.0 1.0
CG A:HIS983 3.2 30.4 1.0
P D:DC34 3.2 44.5 1.0
OG A:SER15 3.6 33.2 1.0
OD1 A:ASP10 3.6 30.0 1.0
CB A:HIS983 3.7 30.4 1.0
CA A:HIS983 3.9 30.4 1.0
NE2 A:HIS983 4.0 30.4 1.0
O A:ASP10 4.1 30.0 1.0
C5' D:DC34 4.1 44.5 1.0
O5' D:DC34 4.1 44.5 1.0
MG A:MG1401 4.2 42.7 1.0
CD2 A:HIS983 4.2 30.4 1.0
CB A:ASP10 4.3 30.0 1.0
OP2 D:DC34 4.3 44.5 1.0
OP1 D:DG35 4.6 55.5 1.0
C A:ASP10 4.7 30.0 1.0
N A:HIS983 4.7 30.4 1.0
CD2 A:HIS982 4.7 30.8 1.0
OD2 A:ASP986 4.7 29.2 1.0
CB A:SER15 4.8 33.2 1.0
OP2 D:DG35 4.8 55.5 1.0
NE2 A:HIS982 4.9 30.8 1.0
C A:HIS983 5.0 30.4 1.0

Magnesium binding site 3 out of 5 in 7s4x

Go back to Magnesium Binding Sites List in 7s4x
Magnesium binding site 3 out of 5 in the CAS9:Grna in Complex with 18-20MM Dna, 1 Minute Time-Point, Kinked Active Conformation


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of CAS9:Grna in Complex with 18-20MM Dna, 1 Minute Time-Point, Kinked Active Conformation within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg1403

b:49.6
occ:1.00
OD1 A:ASP839 2.4 54.6 1.0
OP2 c:DT23 2.5 51.7 1.0
OD1 A:ASN863 3.1 58.8 1.0
CG A:ASP839 3.6 54.6 1.0
O A:HIS840 3.9 51.3 1.0
P c:DT23 3.9 51.7 1.0
NZ A:LYS866 3.9 64.0 1.0
C3' C:DG22 4.0 49.8 1.0
N A:HIS840 4.0 51.3 1.0
CG A:ASN863 4.1 58.8 1.0
O A:LYS866 4.1 64.0 1.0
O3' C:DG22 4.3 49.8 1.0
ND2 A:ASN863 4.4 58.8 1.0
CB A:ASP839 4.4 54.6 1.0
CA A:ASP839 4.4 54.6 1.0
OP3 c:DT23 4.4 51.7 1.0
OD2 A:ASP839 4.5 54.6 1.0
CD A:LYS866 4.5 64.0 1.0
OP1 C:DG22 4.7 49.8 1.0
C A:ASP839 4.7 54.6 1.0
C A:HIS840 4.7 51.3 1.0
C5' C:DG22 4.7 49.8 1.0
O5' C:DG22 4.7 49.8 1.0
O5' c:DT23 4.7 51.7 1.0
CA A:HIS840 4.8 51.3 1.0
CE A:LYS866 4.8 64.0 1.0
C4' C:DG22 4.9 49.8 1.0
C2' C:DG22 4.9 49.8 1.0
OP1 c:DT23 4.9 51.7 1.0
O A:ASN863 4.9 58.8 1.0
CB A:HIS840 5.0 51.3 1.0

Magnesium binding site 4 out of 5 in 7s4x

Go back to Magnesium Binding Sites List in 7s4x
Magnesium binding site 4 out of 5 in the CAS9:Grna in Complex with 18-20MM Dna, 1 Minute Time-Point, Kinked Active Conformation


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of CAS9:Grna in Complex with 18-20MM Dna, 1 Minute Time-Point, Kinked Active Conformation within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg1404

b:45.9
occ:1.00
OG1 A:THR1102 2.7 34.5 1.0
OP2 B:A65 2.8 46.2 1.0
OP1 B:U64 3.6 40.1 1.0
CB A:THR1102 4.0 34.5 1.0
O A:THR1102 4.1 34.5 1.0
P B:A65 4.1 46.2 1.0
OE1 A:GLU57 4.1 46.2 1.0
N A:THR1102 4.2 34.5 1.0
OP1 B:A65 4.4 46.2 1.0
CA A:THR1102 4.5 34.5 1.0
C A:THR1102 4.5 34.5 1.0
CG2 A:THR1102 4.6 34.5 1.0
P B:U64 4.6 40.1 1.0
OE2 A:GLU57 4.8 46.2 1.0
OP2 B:U64 4.8 40.1 1.0
O A:VAL1100 4.8 35.0 1.0
CD A:GLU57 4.9 46.2 1.0
N4 B:C67 4.9 53.2 1.0

Magnesium binding site 5 out of 5 in 7s4x

Go back to Magnesium Binding Sites List in 7s4x
Magnesium binding site 5 out of 5 in the CAS9:Grna in Complex with 18-20MM Dna, 1 Minute Time-Point, Kinked Active Conformation


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of CAS9:Grna in Complex with 18-20MM Dna, 1 Minute Time-Point, Kinked Active Conformation within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg101

b:42.1
occ:1.00
O6 B:G4 4.2 45.6 1.0
OP2 B:A2 4.2 78.9 1.0
O4 c:DT34 4.2 41.9 1.0
O6 c:DG35 4.5 44.0 1.0
C7 c:DT34 4.5 41.9 1.0
N4 B:C5 4.6 38.8 1.0
OP2 B:C3 4.8 51.8 1.0
O5' B:A2 4.8 78.9 1.0
P B:A2 4.9 78.9 1.0
N4 c:DC36 4.9 46.9 1.0

Reference:

J.P.K.Bravo, M.S.Liu, G.N.Hibshman, T.L.Dangerfield, K.Jung, R.S.Mccool, K.A.Johnson, D.W.Taylor. Structural Basis For Mismatch Surveillance By Crispr-CAS9. Nature V. 603 343 2022.
ISSN: ESSN 1476-4687
PubMed: 35236982
DOI: 10.1038/S41586-022-04470-1
Page generated: Thu Oct 3 08:10:39 2024

Last articles

Cl in 2WZN
Cl in 2WZX
Cl in 2WZD
Cl in 2WZQ
Cl in 2WYW
Cl in 2WYT
Cl in 2WZC
Cl in 2WUV
Cl in 2WZB
Cl in 2WYJ
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy