Magnesium in PDB 7sj8: 13PF Wildtype Microtubule From Recombinant Human Tubulin Decorated with Kinesin

The binding sites of Magnesium atom in the 13PF Wildtype Microtubule From Recombinant Human Tubulin Decorated with Kinesin (pdb code 7sj8). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 6 binding sites of Magnesium where determined in the 13PF Wildtype Microtubule From Recombinant Human Tubulin Decorated with Kinesin, PDB code: 7sj8:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6;

Magnesium binding site 1 out of 6 in the 13PF Wildtype Microtubule From Recombinant Human Tubulin Decorated with Kinesin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of 13PF Wildtype Microtubule From Recombinant Human Tubulin Decorated with Kinesin within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg502 b:116.2 occ:1.00 O1B A:GTP501 1.9 109.5 1.0 O3G A:GTP501 2.0 109.5 1.0 O3B A:GTP501 2.0 109.5 1.0 OE2 A:GLU71 2.1 121.0 1.0 PB A:GTP501 2.3 109.5 1.0 PG A:GTP501 2.3 109.5 1.0 O2G A:GTP501 3.1 109.5 1.0 CD A:GLU71 3.2 121.0 1.0 O2B A:GTP501 3.3 109.5 1.0 O3A A:GTP501 3.6 109.5 1.0 O1G A:GTP501 3.7 109.5 1.0 CG A:GLU71 3.8 121.0 1.0 OG1 A:THR145 4.1 109.7 1.0 CB A:ASP98 4.1 127.5 1.0 OD1 A:ASP69 4.1 115.7 1.0 CB A:THR145 4.2 109.7 1.0 OE1 A:GLU71 4.3 121.0 1.0 N A:GLN11 4.6 103.8 1.0 OD2 A:ASP98 4.6 127.5 1.0 OD2 A:ASP69 4.8 115.7 1.0 CB A:GLN11 4.8 103.8 1.0 CG A:ASP69 4.9 115.7 1.0 CG A:ASP98 4.9 127.5 1.0 PA A:GTP501 4.9 109.5 1.0 O1A A:GTP501 5.0 109.5 1.0 N A:THR145 5.0 109.7 1.0

Magnesium binding site 2 out of 6 in the 13PF Wildtype Microtubule From Recombinant Human Tubulin Decorated with Kinesin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of 13PF Wildtype Microtubule From Recombinant Human Tubulin Decorated with Kinesin within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg502 b:131.2 occ:1.00 O1B C:GTP501 1.9 122.7 1.0 O3G C:GTP501 2.0 122.7 1.0 O3B C:GTP501 2.0 122.7 1.0 OE2 C:GLU71 2.1 125.2 1.0 PB C:GTP501 2.3 122.7 1.0 PG C:GTP501 2.3 122.7 1.0 O2G C:GTP501 3.1 122.7 1.0 CD C:GLU71 3.2 125.2 1.0 O2B C:GTP501 3.3 122.7 1.0 O3A C:GTP501 3.6 122.7 1.0 O1G C:GTP501 3.7 122.7 1.0 CG C:GLU71 3.8 125.2 1.0 OG1 C:THR145 4.1 119.8 1.0 CB C:ASP98 4.1 130.4 1.0 OD1 C:ASP69 4.1 118.6 1.0 CB C:THR145 4.2 119.8 1.0 OE1 C:GLU71 4.3 125.2 1.0 N C:GLN11 4.6 114.0 1.0 OD2 C:ASP98 4.6 130.4 1.0 CB C:GLN11 4.8 114.0 1.0 OD2 C:ASP69 4.8 118.6 1.0 CG C:ASP69 4.9 118.6 1.0 CG C:ASP98 4.9 130.4 1.0 PA C:GTP501 4.9 122.7 1.0 O1A C:GTP501 5.0 122.7 1.0 N C:THR145 5.0 119.8 1.0

Magnesium binding site 3 out of 6 in the 13PF Wildtype Microtubule From Recombinant Human Tubulin Decorated with Kinesin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of 13PF Wildtype Microtubule From Recombinant Human Tubulin Decorated with Kinesin within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg502 b:127.6 occ:1.00 O1B E:GTP501 1.9 122.8 1.0 O3G E:GTP501 2.0 122.8 1.0 O3B E:GTP501 2.0 122.8 1.0 OE2 E:GLU71 2.1 133.4 1.0 PB E:GTP501 2.3 122.8 1.0 PG E:GTP501 2.3 122.8 1.0 O2G E:GTP501 3.1 122.8 1.0 CD E:GLU71 3.2 133.4 1.0 O2B E:GTP501 3.3 122.8 1.0 O3A E:GTP501 3.6 122.8 1.0 O1G E:GTP501 3.7 122.8 1.0 CG E:GLU71 3.8 133.4 1.0 OG1 E:THR145 4.1 127.9 1.0 CB E:ASP98 4.1 139.0 1.0 OD1 E:ASP69 4.1 131.5 1.0 CB E:THR145 4.2 127.9 1.0 OE1 E:GLU71 4.3 133.4 1.0 OD2 E:ASP98 4.6 139.0 1.0 N E:GLN11 4.6 118.0 1.0 OD2 E:ASP69 4.8 131.5 1.0 CB E:GLN11 4.8 118.0 1.0 CG E:ASP69 4.9 131.5 1.0 CG E:ASP98 4.9 139.0 1.0 PA E:GTP501 4.9 122.8 1.0 O1A E:GTP501 5.0 122.8 1.0 N E:THR145 5.0 127.9 1.0

Magnesium binding site 4 out of 6 in the 13PF Wildtype Microtubule From Recombinant Human Tubulin Decorated with Kinesin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of 13PF Wildtype Microtubule From Recombinant Human Tubulin Decorated with Kinesin within 5.0Å range: probe atom residue distance (Å) B Occ J:Mg502 b:111.5 occ:1.00 O1B J:GTP501 1.9 104.3 1.0 O3G J:GTP501 2.0 104.3 1.0 O3B J:GTP501 2.0 104.3 1.0 OE2 J:GLU71 2.1 111.0 1.0 PB J:GTP501 2.3 104.3 1.0 PG J:GTP501 2.3 104.3 1.0 O2G J:GTP501 3.1 104.3 1.0 CD J:GLU71 3.2 111.0 1.0 O2B J:GTP501 3.3 104.3 1.0 O3A J:GTP501 3.6 104.3 1.0 O1G J:GTP501 3.7 104.3 1.0 CG J:GLU71 3.8 111.0 1.0 OG1 J:THR145 4.1 107.0 1.0 CB J:ASP98 4.1 114.2 1.0 OD1 J:ASP69 4.1 109.3 1.0 CB J:THR145 4.2 107.0 1.0 OE1 J:GLU71 4.3 111.0 1.0 OD2 J:ASP98 4.6 114.2 1.0 N J:GLN11 4.6 100.9 1.0 OD2 J:ASP69 4.8 109.3 1.0 CB J:GLN11 4.8 100.9 1.0 CG J:ASP69 4.9 109.3 1.0 CG J:ASP98 4.9 114.2 1.0 PA J:GTP501 4.9 104.3 1.0 O1A J:GTP501 5.0 104.3 1.0 N J:THR145 5.0 107.0 1.0

Magnesium binding site 5 out of 6 in the 13PF Wildtype Microtubule From Recombinant Human Tubulin Decorated with Kinesin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of 13PF Wildtype Microtubule From Recombinant Human Tubulin Decorated with Kinesin within 5.0Å range: probe atom residue distance (Å) B Occ K:Mg502 b:97.6 occ:1.00 O1B K:GTP501 1.9 89.7 1.0 O3G K:GTP501 2.0 89.7 1.0 O3B K:GTP501 2.0 89.7 1.0 OE2 K:GLU71 2.1 95.0 1.0 PB K:GTP501 2.3 89.7 1.0 PG K:GTP501 2.3 89.7 1.0 O2G K:GTP501 3.1 89.7 1.0 CD K:GLU71 3.2 95.0 1.0 O2B K:GTP501 3.3 89.7 1.0 O3A K:GTP501 3.6 89.7 1.0 O1G K:GTP501 3.7 89.7 1.0 CG K:GLU71 3.8 95.0 1.0 OG1 K:THR145 4.1 92.0 1.0 CB K:ASP98 4.1 101.0 1.0 OD1 K:ASP69 4.1 91.4 1.0 CB K:THR145 4.2 92.0 1.0 OE1 K:GLU71 4.3 95.0 1.0 OD2 K:ASP98 4.6 101.0 1.0 N K:GLN11 4.6 85.7 1.0 OD2 K:ASP69 4.8 91.4 1.0 CB K:GLN11 4.8 85.7 1.0 CG K:ASP69 4.9 91.4 1.0 CG K:ASP98 4.9 101.0 1.0 PA K:GTP501 4.9 89.7 1.0 O1A K:GTP501 5.0 89.7 1.0 N K:THR145 5.0 92.0 1.0

Magnesium binding site 6 out of 6 in the 13PF Wildtype Microtubule From Recombinant Human Tubulin Decorated with Kinesin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of 13PF Wildtype Microtubule From Recombinant Human Tubulin Decorated with Kinesin within 5.0Å range: probe atom residue distance (Å) B Occ L:Mg502 b:120.8 occ:1.00 O1B L:GTP501 1.9 108.0 1.0 O3G L:GTP501 2.0 108.0 1.0 O3B L:GTP501 2.0 108.0 1.0 OE2 L:GLU71 2.1 109.7 1.0 PB L:GTP501 2.3 108.0 1.0 PG L:GTP501 2.3 108.0 1.0 O2G L:GTP501 3.1 108.0 1.0 CD L:GLU71 3.2 109.7 1.0 O2B L:GTP501 3.3 108.0 1.0 O3A L:GTP501 3.6 108.0 1.0 O1G L:GTP501 3.7 108.0 1.0 CG L:GLU71 3.8 109.7 1.0 OG1 L:THR145 4.1 107.5 1.0 CB L:ASP98 4.1 112.7 1.0 OD1 L:ASP69 4.1 104.4 1.0 CB L:THR145 4.2 107.5 1.0 OE1 L:GLU71 4.3 109.7 1.0 OD2 L:ASP98 4.6 112.7 1.0 N L:GLN11 4.6 98.5 1.0 OD2 L:ASP69 4.8 104.4 1.0 CB L:GLN11 4.8 98.5 1.0 CG L:ASP69 4.9 104.4 1.0 CG L:ASP98 4.9 112.7 1.0 PA L:GTP501 4.9 108.0 1.0 O1A L:GTP501 5.0 108.0 1.0 N L:THR145 5.0 107.5 1.0

B.J.Lafrance, J.Roostalu, G.Henkin, B.J.Greber, R.Zhang, D.Normanno, C.O.Mccollum, T.Surrey, E.Nogales. Structural Transitions in the Gtp Cap Visualized By Cryo-Electron Microscopy of Catalytically Inactive Microtubules. Proc.Natl.Acad.Sci.Usa V. 119 2022.
ISSN: ESSN 1091-6490
PubMed: 34996871
DOI: 10.1073/PNAS.2114994119