Magnesium in PDB 7sj9: 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3

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>>> Page 1 <<< Page 2, Binding sites: 11 - 12;

Binding sites:

The binding sites of Magnesium atom in the 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3 (pdb code 7sj9). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 12 binding sites of Magnesium where determined in the 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3, PDB code: 7sj9:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Magnesium binding site 1 out of 12 in the 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg502 b:98.4 occ:1.00 O1B A:GTP501 2.1 105.1 1.0 O3B A:GTP501 2.1 105.1 1.0 O3G A:GTP501 2.2 105.1 1.0 PG A:GTP501 2.5 105.1 1.0 PB A:GTP501 2.5 105.1 1.0 OE2 A:GLU71 3.1 113.5 1.0 O2G A:GTP501 3.2 105.1 1.0 CD A:GLU71 3.2 113.5 1.0 O2B A:GTP501 3.4 105.1 1.0 CG A:GLU71 3.6 113.5 1.0 OG1 A:THR145 3.6 106.3 1.0 OE1 A:GLU71 3.7 113.5 1.0 O3A A:GTP501 3.8 105.1 1.0 O1G A:GTP501 3.9 105.1 1.0 CB A:ASP98 4.0 118.8 1.0 OD1 A:ASP69 4.1 106.1 1.0 CB A:THR145 4.4 106.3 1.0 OD2 A:ASP69 4.4 106.1 1.0 CB A:GLU71 4.5 113.5 1.0 CG A:ASP69 4.7 106.1 1.0 CG A:ASP98 4.7 118.8 1.0 NZ H:LYS252 4.7 110.7 1.0 OD2 A:ASP98 4.8 118.8 1.0 CB A:GLN11 4.9 97.6 1.0 OE1 A:GLN11 4.9 97.6 1.0 N A:GLN11 5.0 97.6 1.0

Magnesium binding site 2 out of 12 in the 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3 within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg502 b:99.8 occ:1.00 O3B B:GTP501 2.1 99.3 1.0 O3G B:GTP501 2.2 99.3 1.0 O1B B:GTP501 2.3 99.3 1.0 PB B:GTP501 2.5 99.3 1.0 PG B:GTP501 2.6 99.3 1.0 OE2 B:GLU69 3.1 102.0 1.0 CD B:GLU69 3.1 102.0 1.0 O2B B:GTP501 3.2 99.3 1.0 O2G B:GTP501 3.5 99.3 1.0 OE1 B:GLU69 3.5 102.0 1.0 CG B:GLU69 3.7 102.0 1.0 O1G B:GTP501 3.8 99.3 1.0 OG1 B:THR143 3.8 100.4 1.0 O3A B:GTP501 4.0 99.3 1.0 OD1 B:ASP67 4.1 99.5 1.0 CB B:THR143 4.4 100.4 1.0 CB B:GLU69 4.6 102.0 1.0 OD2 B:ASP67 4.7 99.5 1.0 CG B:ASP67 4.9 99.5 1.0

Magnesium binding site 3 out of 12 in the 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3 within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg502 b:122.2 occ:1.00 O1B C:GTP501 2.1 122.4 1.0 O3B C:GTP501 2.1 122.4 1.0 O3G C:GTP501 2.1 122.4 1.0 PG C:GTP501 2.5 122.4 1.0 PB C:GTP501 2.5 122.4 1.0 OE2 C:GLU71 3.0 122.4 1.0 CD C:GLU71 3.1 122.4 1.0 O2G C:GTP501 3.1 122.4 1.0 O2B C:GTP501 3.4 122.4 1.0 OE1 C:GLU71 3.4 122.4 1.0 CG C:GLU71 3.6 122.4 1.0 OG1 C:THR145 3.6 119.4 1.0 O3A C:GTP501 3.8 122.4 1.0 OD1 C:ASP69 3.8 115.8 1.0 O1G C:GTP501 3.9 122.4 1.0 CB C:ASP98 3.9 135.1 1.0 OD2 C:ASP69 4.3 115.8 1.0 CB C:THR145 4.4 119.4 1.0 CB C:GLU71 4.4 122.4 1.0 CG C:ASP69 4.5 115.8 1.0 OD2 C:ASP98 4.5 135.1 1.0 NZ I:LYS252 4.6 128.0 1.0 CG C:ASP98 4.8 135.1 1.0 CB C:GLN11 4.9 115.9 1.0 OE1 C:GLN11 5.0 115.9 1.0

Magnesium binding site 4 out of 12 in the 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3 within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg502 b:117.8 occ:1.00 O3B D:GTP501 2.1 118.1 1.0 O1B D:GTP501 2.2 118.1 1.0 O3G D:GTP501 2.3 118.1 1.0 PB D:GTP501 2.4 118.1 1.0 PG D:GTP501 2.6 118.1 1.0 O2B D:GTP501 3.1 118.1 1.0 O2G D:GTP501 3.5 118.1 1.0 OG1 D:THR143 3.5 115.4 1.0 OE1 D:GLU69 3.9 110.8 1.0 O1G D:GTP501 3.9 118.1 1.0 O3A D:GTP501 3.9 118.1 1.0 CB D:THR143 4.2 115.4 1.0 OD1 D:ASP67 4.3 108.3 1.0 CB D:GLU69 4.8 110.8 1.0 CG2 D:THR143 4.8 115.4 1.0 OD2 D:ASP67 4.9 108.3 1.0

Magnesium binding site 5 out of 12 in the 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3 within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg502 b:117.8 occ:1.00 O1B E:GTP501 2.1 119.3 1.0 O3B E:GTP501 2.1 119.3 1.0 O3G E:GTP501 2.2 119.3 1.0 PB E:GTP501 2.5 119.3 1.0 PG E:GTP501 2.5 119.3 1.0 OE2 E:GLU71 3.0 127.0 1.0 CD E:GLU71 3.1 127.0 1.0 O2G E:GTP501 3.2 119.3 1.0 O2B E:GTP501 3.4 119.3 1.0 OE1 E:GLU71 3.4 127.0 1.0 OG1 E:THR145 3.6 122.9 1.0 CG E:GLU71 3.7 127.0 1.0 O3A E:GTP501 3.8 119.3 1.0 OD1 E:ASP69 3.9 122.7 1.0 O1G E:GTP501 3.9 119.3 1.0 CB E:ASP98 3.9 132.5 1.0 CB E:THR145 4.4 122.9 1.0 OD2 E:ASP69 4.4 122.7 1.0 CB E:GLU71 4.5 127.0 1.0 CG E:ASP69 4.6 122.7 1.0 CG E:ASP98 4.6 132.5 1.0 OD2 E:ASP98 4.7 132.5 1.0 NZ G:LYS252 4.8 126.9 1.0 CB E:GLN11 4.9 115.8 1.0 OE1 E:GLN11 4.9 115.8 1.0

Magnesium binding site 6 out of 12 in the 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3 within 5.0Å range: probe atom residue distance (Å) B Occ F:Mg502 b:109.4 occ:1.00 O3B F:GTP501 2.1 112.5 1.0 O3G F:GTP501 2.1 112.5 1.0 O1B F:GTP501 2.3 112.5 1.0 PB F:GTP501 2.5 112.5 1.0 PG F:GTP501 2.6 112.5 1.0 O2B F:GTP501 3.2 112.5 1.0 OE2 F:GLU69 3.2 117.6 1.0 CD F:GLU69 3.3 117.6 1.0 O2G F:GTP501 3.5 112.5 1.0 OE1 F:GLU69 3.7 117.6 1.0 O1G F:GTP501 3.8 112.5 1.0 OG1 F:THR143 3.9 114.7 1.0 CG F:GLU69 3.9 117.6 1.0 O3A F:GTP501 4.0 112.5 1.0 OD1 F:ASP67 4.1 119.6 1.0 CB F:THR143 4.5 114.7 1.0 OD2 F:ASP67 4.7 119.6 1.0 CB F:GLU69 4.7 117.6 1.0 CG F:ASP67 4.8 119.6 1.0

Magnesium binding site 7 out of 12 in the 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 7 of 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3 within 5.0Å range: probe atom residue distance (Å) B Occ G:Mg502 b:173.2 occ:1.00 O3B G:GTP501 2.1 165.7 1.0 O1B G:GTP501 2.1 165.7 1.0 O3G G:GTP501 2.3 165.7 1.0 PB G:GTP501 2.4 165.7 1.0 PG G:GTP501 2.6 165.7 1.0 O2B G:GTP501 3.1 165.7 1.0 O2G G:GTP501 3.5 165.7 1.0 OG1 G:THR143 3.6 167.5 1.0 O1G G:GTP501 3.9 165.7 1.0 O3A G:GTP501 3.9 165.7 1.0 CB G:THR143 4.2 167.5 1.0 OD1 G:ASP67 4.3 159.9 1.0 OD2 G:ASP67 4.8 159.9 1.0 CG2 G:THR143 4.9 167.5 1.0 CB G:GLU69 4.9 161.9 1.0

Magnesium binding site 8 out of 12 in the 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 8 of 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3 within 5.0Å range: probe atom residue distance (Å) B Occ H:Mg502 b:157.4 occ:1.00 O1B H:GTP501 2.1 147.6 1.0 O3B H:GTP501 2.1 147.6 1.0 O3G H:GTP501 2.3 147.6 1.0 PB H:GTP501 2.4 147.6 1.0 PG H:GTP501 2.7 147.6 1.0 O2B H:GTP501 3.1 147.6 1.0 OE2 H:GLU69 3.5 145.5 1.0 O2G H:GTP501 3.5 147.6 1.0 OG1 H:THR143 3.6 142.1 1.0 CD H:GLU69 3.6 145.5 1.0 OE1 H:GLU69 3.9 145.5 1.0 O3A H:GTP501 3.9 147.6 1.0 O1G H:GTP501 3.9 147.6 1.0 CB H:THR143 4.2 142.1 1.0 CG H:GLU69 4.2 145.5 1.0 OD1 H:ASP67 4.3 137.6 1.0 CG2 H:THR143 4.9 142.1 1.0 OD2 H:ASP67 4.9 137.6 1.0 CB H:GLU69 5.0 145.5 1.0

Magnesium binding site 9 out of 12 in the 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 9 of 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3 within 5.0Å range: probe atom residue distance (Å) B Occ I:Mg502 b:184.3 occ:1.00 O3B I:GTP501 2.1 172.3 1.0 O1B I:GTP501 2.1 172.3 1.0 O3G I:GTP501 2.3 172.3 1.0 PB I:GTP501 2.4 172.3 1.0 PG I:GTP501 2.6 172.3 1.0 O2B I:GTP501 3.1 172.3 1.0 O2G I:GTP501 3.5 172.3 1.0 OG1 I:THR143 3.6 160.1 1.0 O1G I:GTP501 3.9 172.3 1.0 O3A I:GTP501 3.9 172.3 1.0 CB I:THR143 4.2 160.1 1.0 OD1 I:ASP67 4.3 148.5 1.0 OD2 I:ASP67 4.9 148.5 1.0 CG2 I:THR143 4.9 160.1 1.0 CB I:GLU69 4.9 159.3 1.0

Magnesium binding site 10 out of 12 in the 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 10 of 13PF E254A Microtubule From Recombinant Human Tubulin Decorated with EB3 within 5.0Å range: probe atom residue distance (Å) B Occ J:Mg502 b:150.1 occ:1.00 O3B J:GTP501 2.1 151.8 1.0 OE2 J:GLU71 2.2 152.5 1.0 O1B J:GTP501 2.2 151.8 1.0 O3G J:GTP501 2.3 151.8 1.0 PB J:GTP501 2.5 151.8 1.0 PG J:GTP501 2.5 151.8 1.0 CD J:GLU71 2.8 152.5 1.0 O2G J:GTP501 3.2 151.8 1.0 CG J:GLU71 3.3 152.5 1.0 O2B J:GTP501 3.3 151.8 1.0 OE1 J:GLU71 3.7 152.5 1.0 OG1 J:THR145 3.7 157.6 1.0 CG2 J:THR145 3.8 157.6 1.0 CB J:ASP98 3.9 151.1 1.0 O3A J:GTP501 3.9 151.8 1.0 O1G J:GTP501 3.9 151.8 1.0 OD1 J:ASP69 4.0 156.7 1.0 CB J:THR145 4.4 157.6 1.0 OD2 J:ASP69 4.4 156.7 1.0 OD2 J:ASP98 4.5 151.1 1.0 CG J:ASP69 4.6 156.7 1.0 CB J:GLU71 4.6 152.5 1.0 CG J:ASP98 4.8 151.1 1.0 CB J:GLN11 4.8 146.5 1.0 OE1 J:GLN11 4.9 146.5 1.0 N J:GLN11 4.9 146.5 1.0 CA J:ASP98 5.0 151.1 1.0

B.J.Lafrance, J.Roostalu, G.Henkin, B.J.Greber, R.Zhang, D.Normanno, C.O.Mccollum, T.Surrey, E.Nogales. Structural Transitions in the Gtp Cap Visualized By Cryo-Electron Microscopy of Catalytically Inactive Microtubules. Proc.Natl.Acad.Sci.Usa V. 119 2022.
ISSN: ESSN 1091-6490
PubMed: 34996871
DOI: 10.1073/PNAS.2114994119