Magnesium in PDB 7sja: Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin

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>>> Page 1 <<< Page 2, Binding sites: 11 - 12;

Binding sites:

The binding sites of Magnesium atom in the Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin (pdb code 7sja). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 12 binding sites of Magnesium where determined in the Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin, PDB code: 7sja:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Magnesium binding site 1 out of 12 in the Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg502 b:127.5 occ:1.00 O3B A:GTP501 2.0 116.9 1.0 O1B A:GTP501 2.1 116.9 1.0 PB A:GTP501 2.3 116.9 1.0 OD2 A:ASP98 2.5 131.6 1.0 O3G A:GTP501 2.7 116.9 1.0 PG A:GTP501 2.7 116.9 1.0 CB A:ASP98 2.8 131.6 1.0 OE2 A:GLU71 2.8 128.7 1.0 O2B A:GTP501 2.9 116.9 1.0 CG A:ASP98 3.0 131.6 1.0 CD A:GLU71 3.3 128.7 1.0 O2G A:GTP501 3.3 116.9 1.0 NZ H:LYS252 3.7 125.8 1.0 OG1 A:THR145 3.7 123.1 1.0 O3A A:GTP501 3.8 116.9 1.0 OE1 A:GLU71 3.8 128.7 1.0 CG A:GLU71 4.0 128.7 1.0 O1G A:GTP501 4.0 116.9 1.0 CA A:ASP98 4.1 131.6 1.0 OD1 A:ASP98 4.3 131.6 1.0 N A:ALA100 4.4 127.6 1.0 N A:ALA99 4.5 128.6 1.0 C A:ASP98 4.6 131.6 1.0 CB A:GLU71 4.7 128.7 1.0 CE H:LYS252 4.7 125.8 1.0 CD H:LYS252 4.7 125.8 1.0 CB A:THR145 4.8 123.1 1.0 CA A:ALA100 4.9 127.6 1.0 OD1 A:ASP69 4.9 122.6 1.0

Magnesium binding site 2 out of 12 in the Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg502 b:78.6 occ:1.00 O1B B:GTP501 2.0 73.8 1.0 O3B B:GTP501 2.0 73.8 1.0 PB B:GTP501 2.2 73.8 1.0 O2B B:GTP501 2.8 73.8 1.0 O3G B:GTP501 2.9 73.8 1.0 PG B:GTP501 2.9 73.8 1.0 OE1 B:GLU69 3.1 85.8 1.0 OE2 B:GLU69 3.2 85.8 1.0 CD B:GLU69 3.4 85.8 1.0 O2G B:GTP501 3.6 73.8 1.0 OG1 B:THR143 3.7 81.0 1.0 O3A B:GTP501 3.8 73.8 1.0 O1G B:GTP501 4.2 73.8 1.0 CG B:GLU69 4.6 85.8 1.0 CA B:GLY96 4.6 83.6 1.0 CB B:THR143 4.7 81.0 1.0 N B:GLY98 4.7 79.0 1.0 N B:ALA97 4.7 77.2 1.0 OD1 B:ASP67 4.8 84.4 1.0 C B:GLY96 4.9 83.6 1.0 O1A B:GTP501 4.9 73.8 1.0 PA B:GTP501 5.0 73.8 1.0

Magnesium binding site 3 out of 12 in the Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg502 b:145.0 occ:1.00 O3B C:GTP501 2.0 137.2 1.0 O1B C:GTP501 2.1 137.2 1.0 PB C:GTP501 2.3 137.2 1.0 OD2 C:ASP98 2.5 145.1 1.0 O3G C:GTP501 2.7 137.2 1.0 PG C:GTP501 2.7 137.2 1.0 CB C:ASP98 2.8 145.1 1.0 OE2 C:GLU71 2.8 140.2 1.0 O2B C:GTP501 2.9 137.2 1.0 CG C:ASP98 3.0 145.1 1.0 CD C:GLU71 3.3 140.2 1.0 O2G C:GTP501 3.3 137.2 1.0 OG1 C:THR145 3.7 141.2 1.0 O3A C:GTP501 3.8 137.2 1.0 OE1 C:GLU71 3.8 140.2 1.0 NZ I:LYS252 3.8 142.9 1.0 CG C:GLU71 4.0 140.2 1.0 O1G C:GTP501 4.0 137.2 1.0 CA C:ASP98 4.1 145.1 1.0 OD1 C:ASP98 4.3 145.1 1.0 N C:ALA100 4.4 143.2 1.0 N C:ALA99 4.5 143.7 1.0 C C:ASP98 4.6 145.1 1.0 CB C:GLU71 4.7 140.2 1.0 CB C:THR145 4.8 141.2 1.0 CA C:ALA100 4.9 143.2 1.0 CE I:LYS252 4.9 142.9 1.0 OD1 C:ASP69 4.9 136.4 1.0 CD I:LYS252 5.0 142.9 1.0

Magnesium binding site 4 out of 12 in the Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg502 b:98.0 occ:1.00 O1B D:GTP501 2.0 90.3 1.0 O3B D:GTP501 2.0 90.3 1.0 PB D:GTP501 2.2 90.3 1.0 O2B D:GTP501 2.8 90.3 1.0 O3G D:GTP501 2.9 90.3 1.0 PG D:GTP501 2.9 90.3 1.0 OE1 D:GLU69 3.1 96.9 1.0 OE2 D:GLU69 3.2 96.9 1.0 CD D:GLU69 3.4 96.9 1.0 O2G D:GTP501 3.6 90.3 1.0 OG1 D:THR143 3.7 94.9 1.0 O3A D:GTP501 3.8 90.3 1.0 O1G D:GTP501 4.2 90.3 1.0 CG D:GLU69 4.6 96.9 1.0 CA D:GLY96 4.6 92.1 1.0 CB D:THR143 4.7 94.9 1.0 N D:GLY98 4.7 94.5 1.0 N D:ALA97 4.7 90.6 1.0 OD1 D:ASP67 4.8 95.8 1.0 OD2 C:ASP251 4.8 105.7 1.0 C D:GLY96 4.9 92.1 1.0 O1A D:GTP501 4.9 90.3 1.0 PA D:GTP501 5.0 90.3 1.0

Magnesium binding site 5 out of 12 in the Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg502 b:141.4 occ:1.00 O3B E:GTP501 2.0 131.4 1.0 O1B E:GTP501 2.1 131.4 1.0 PB E:GTP501 2.3 131.4 1.0 OD2 E:ASP98 2.5 145.3 1.0 O3G E:GTP501 2.7 131.4 1.0 PG E:GTP501 2.7 131.4 1.0 CB E:ASP98 2.8 145.3 1.0 OE2 E:GLU71 2.8 144.7 1.0 O2B E:GTP501 2.9 131.4 1.0 CG E:ASP98 3.0 145.3 1.0 CD E:GLU71 3.3 144.7 1.0 O2G E:GTP501 3.3 131.4 1.0 OG1 E:THR145 3.7 138.3 1.0 NZ G:LYS252 3.7 138.1 1.0 O3A E:GTP501 3.8 131.4 1.0 OE1 E:GLU71 3.8 144.7 1.0 CG E:GLU71 4.0 144.7 1.0 O1G E:GTP501 4.0 131.4 1.0 CA E:ASP98 4.1 145.3 1.0 OD1 E:ASP98 4.3 145.3 1.0 N E:ALA100 4.4 138.2 1.0 N E:ALA99 4.5 140.0 1.0 C E:ASP98 4.6 145.3 1.0 CB E:GLU71 4.7 144.7 1.0 CE G:LYS252 4.7 138.1 1.0 CD G:LYS252 4.8 138.1 1.0 CB E:THR145 4.8 138.3 1.0 CA E:ALA100 4.9 138.2 1.0 OD1 E:ASP69 4.9 142.6 1.0

Magnesium binding site 6 out of 12 in the Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin within 5.0Å range: probe atom residue distance (Å) B Occ F:Mg502 b:103.8 occ:1.00 O1B F:GTP501 2.0 94.4 1.0 O3B F:GTP501 2.0 94.4 1.0 PB F:GTP501 2.2 94.4 1.0 O2B F:GTP501 2.8 94.4 1.0 O3G F:GTP501 2.9 94.4 1.0 PG F:GTP501 2.9 94.4 1.0 OE1 F:GLU69 3.1 102.9 1.0 OE2 F:GLU69 3.2 102.9 1.0 CD F:GLU69 3.4 102.9 1.0 O2G F:GTP501 3.6 94.4 1.0 OG1 F:THR143 3.7 101.7 1.0 O3A F:GTP501 3.8 94.4 1.0 O1G F:GTP501 4.2 94.4 1.0 CG F:GLU69 4.6 102.9 1.0 CA F:GLY96 4.6 104.3 1.0 CB F:THR143 4.7 101.7 1.0 N F:GLY98 4.7 96.2 1.0 N F:ALA97 4.7 94.5 1.0 OD1 F:ASP67 4.8 102.7 1.0 C F:GLY96 4.9 104.3 1.0 O1A F:GTP501 4.9 94.4 1.0 PA F:GTP501 5.0 94.4 1.0 OD2 E:ASP251 5.0 108.3 1.0

Magnesium binding site 7 out of 12 in the Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 7 of Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin within 5.0Å range: probe atom residue distance (Å) B Occ G:Mg502 b:190.9 occ:1.00 O1B G:GTP501 2.0 185.4 1.0 O3B G:GTP501 2.0 185.4 1.0 PB G:GTP501 2.2 185.4 1.0 O2B G:GTP501 2.8 185.4 1.0 O3G G:GTP501 2.9 185.4 1.0 PG G:GTP501 2.9 185.4 1.0 OE1 G:GLU69 3.1 194.4 1.0 OE2 G:GLU69 3.2 194.4 1.0 CD G:GLU69 3.4 194.4 1.0 O2G G:GTP501 3.6 185.4 1.0 OG1 G:THR143 3.7 194.9 1.0 O3A G:GTP501 3.8 185.4 1.0 O1G G:GTP501 4.2 185.4 1.0 CG G:GLU69 4.6 194.4 1.0 CA G:GLY96 4.6 198.8 1.0 CB G:THR143 4.7 194.9 1.0 N G:GLY98 4.7 194.7 1.0 N G:ALA97 4.7 194.8 1.0 OD1 G:ASP67 4.8 196.2 1.0 C G:GLY96 4.9 198.8 1.0 O1A G:GTP501 4.9 185.4 1.0 PA G:GTP501 5.0 185.4 1.0

Magnesium binding site 8 out of 12 in the Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 8 of Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin within 5.0Å range: probe atom residue distance (Å) B Occ H:Mg502 b:161.2 occ:1.00 O1B H:GTP501 2.0 152.7 1.0 O3B H:GTP501 2.0 152.7 1.0 PB H:GTP501 2.2 152.7 1.0 O2B H:GTP501 2.8 152.7 1.0 O3G H:GTP501 2.9 152.7 1.0 PG H:GTP501 2.9 152.7 1.0 OE1 H:GLU69 3.1 170.1 1.0 OE2 H:GLU69 3.2 170.1 1.0 CD H:GLU69 3.4 170.1 1.0 O2G H:GTP501 3.6 152.7 1.0 OG1 H:THR143 3.7 162.6 1.0 O3A H:GTP501 3.8 152.7 1.0 O1G H:GTP501 4.2 152.7 1.0 CG H:GLU69 4.6 170.1 1.0 CA H:GLY96 4.6 174.0 1.0 CB H:THR143 4.7 162.6 1.0 N H:GLY98 4.7 169.8 1.0 N H:ALA97 4.7 170.3 1.0 OD1 H:ASP67 4.8 165.7 1.0 C H:GLY96 4.9 174.0 1.0 O1A H:GTP501 4.9 152.7 1.0 PA H:GTP501 5.0 152.7 1.0

Magnesium binding site 9 out of 12 in the Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 9 of Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin within 5.0Å range: probe atom residue distance (Å) B Occ I:Mg502 b:182.8 occ:1.00 O1B I:GTP501 2.0 178.9 1.0 O3B I:GTP501 2.0 178.9 1.0 PB I:GTP501 2.2 178.9 1.0 O2B I:GTP501 2.8 178.9 1.0 O3G I:GTP501 2.9 178.9 1.0 PG I:GTP501 2.9 178.9 1.0 OE1 I:GLU69 3.1 179.6 1.0 OE2 I:GLU69 3.2 179.6 1.0 CD I:GLU69 3.4 179.6 1.0 O2G I:GTP501 3.6 178.9 1.0 OG1 I:THR143 3.7 179.3 1.0 O3A I:GTP501 3.8 178.9 1.0 O1G I:GTP501 4.2 178.9 1.0 CG I:GLU69 4.6 179.6 1.0 CA I:GLY96 4.6 180.9 1.0 CB I:THR143 4.7 179.3 1.0 N I:GLY98 4.7 187.3 1.0 N I:ALA97 4.7 182.6 1.0 OD1 I:ASP67 4.8 176.9 1.0 C I:GLY96 4.9 180.9 1.0 O1A I:GTP501 4.9 178.9 1.0 PA I:GTP501 5.0 178.9 1.0

Magnesium binding site 10 out of 12 in the Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 10 of Undecorated 13PF E254N Microtubule From Recombinant Human Tubulin within 5.0Å range: probe atom residue distance (Å) B Occ J:Mg502 b:103.4 occ:1.00 O3B J:GTP501 2.0 98.8 1.0 O1B J:GTP501 2.1 98.8 1.0 PB J:GTP501 2.3 98.8 1.0 OD2 J:ASP98 2.5 104.7 1.0 O3G J:GTP501 2.7 98.8 1.0 PG J:GTP501 2.7 98.8 1.0 CB J:ASP98 2.8 104.7 1.0 OE2 J:GLU71 2.8 107.7 1.0 O2B J:GTP501 2.9 98.8 1.0 CG J:ASP98 3.0 104.7 1.0 CD J:GLU71 3.3 107.7 1.0 O2G J:GTP501 3.3 98.8 1.0 NZ F:LYS252 3.6 92.0 1.0 OG1 J:THR145 3.7 107.0 1.0 O3A J:GTP501 3.8 98.8 1.0 OE1 J:GLU71 3.8 107.7 1.0 CG J:GLU71 4.0 107.7 1.0 O1G J:GTP501 4.0 98.8 1.0 CA J:ASP98 4.1 104.7 1.0 OD1 J:ASP98 4.3 104.7 1.0 N J:ALA100 4.4 99.9 1.0 N J:ALA99 4.5 99.6 1.0 C J:ASP98 4.6 104.7 1.0 CE F:LYS252 4.6 92.0 1.0 CD F:LYS252 4.7 92.0 1.0 CB J:GLU71 4.7 107.7 1.0 CB J:THR145 4.8 107.0 1.0 CA J:ALA100 4.9 99.9 1.0 OD1 J:ASP69 4.9 110.5 1.0

B.J.Lafrance, J.Roostalu, G.Henkin, B.J.Greber, R.Zhang, D.Normanno, C.O.Mccollum, T.Surrey, E.Nogales. Structural Transitions in the Gtp Cap Visualized By Cryo-Electron Microscopy of Catalytically Inactive Microtubules. Proc.Natl.Acad.Sci.Usa V. 119 2022.
ISSN: ESSN 1091-6490
PubMed: 34996871
DOI: 10.1073/PNAS.2114994119