Magnesium in PDB 7tam: Hras G12V in Complex with Gdp

All present enzymatic activity of Hras G12V in Complex with Gdp:
3.6.5.2;

The structure of Hras G12V in Complex with Gdp, PDB code: 7tam was solved by I.Kouidmi, M.Maddalena, S.Laplante, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.19 / 1.87
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	92.35, 92.35, 120.58, 90, 90, 120
R / Rfree (%)	17.4 / 20.1

The structure of Hras G12V in Complex with Gdp also contains other interesting chemical elements:

Calcium

(Ca)

1 atom

The binding sites of Magnesium atom in the Hras G12V in Complex with Gdp (pdb code 7tam). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Hras G12V in Complex with Gdp, PDB code: 7tam:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Hras G12V in Complex with Gdp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Hras G12V in Complex with Gdp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg202 b:22.1 occ:1.00 O A:HOH341 2.1 27.3 1.0 O2B A:GDP201 2.1 23.0 1.0 O A:HOH322 2.2 26.0 1.0 OG A:SER17 2.2 23.2 1.0 O A:HOH331 2.2 25.7 1.0 O A:HOH351 2.3 34.1 1.0 CB A:SER17 3.2 23.3 1.0 PB A:GDP201 3.4 24.2 1.0 O3B A:GDP201 3.6 26.2 1.0 N A:SER17 4.0 24.2 1.0 CA A:SER17 4.1 24.6 1.0 O2A A:GDP201 4.2 25.4 1.0 OD2 A:ASP57 4.2 28.0 1.0 O A:PRO34 4.2 39.9 1.0 OD1 A:ASP57 4.3 24.7 1.0 CD2 A:TYR32 4.3 35.0 1.0 O A:ASP33 4.4 35.6 1.0 O3A A:GDP201 4.4 24.3 1.0 O1B A:GDP201 4.4 23.4 1.0 CA A:PRO34 4.4 36.5 1.0 O A:ILE36 4.5 31.7 1.0 C A:PRO34 4.6 39.5 1.0 CG A:ASP57 4.6 25.8 1.0 PA A:GDP201 4.6 26.5 1.0 O A:THR58 4.6 28.7 1.0 CB A:ALA59 4.7 34.9 1.0 O1A A:GDP201 4.7 24.8 1.0 CE2 A:TYR32 4.8 37.8 1.0 O A:TYR32 5.0 37.6 1.0

Magnesium binding site 2 out of 2 in the Hras G12V in Complex with Gdp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Hras G12V in Complex with Gdp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg203 b:30.0 occ:0.50 OD1 A:ASP105 1.8 28.3 0.4 O A:ARG102 2.5 32.4 0.6 O A:ARG102 2.5 33.3 0.5 O A:HOH316 2.7 56.7 1.0 CG A:ASP105 2.8 29.9 0.4 OD2 A:ASP105 3.2 29.8 0.4 OD1 A:ASP105 3.6 37.7 0.6 C A:ARG102 3.6 34.4 0.6 C A:ARG102 3.6 34.6 0.5 CG A:ASP105 4.0 37.1 0.6 CB A:ASP105 4.1 31.7 0.4 CB A:ASP105 4.1 35.8 0.6 CA A:ARG102 4.2 33.5 0.5 CG A:ARG102 4.2 40.6 0.5 CA A:ARG102 4.2 32.7 0.6 CG A:ARG102 4.2 38.9 0.6 CA A:ASP105 4.4 36.3 0.6 CA A:ASP105 4.5 33.5 0.4 N A:ASP105 4.6 35.4 0.6 N A:ASP105 4.6 34.4 0.4 N A:VAL103 4.6 32.8 1.0 CD A:ARG102 4.7 41.8 0.5 CB A:ARG102 4.7 34.0 0.5 CB A:ARG102 4.8 32.7 0.6 CA A:VAL103 4.9 32.8 1.0 OD2 A:ASP105 4.9 36.1 0.6

I.Kouidmi, M.Maddalena, S.Laplante. Structure of Hras G12V in Complex with Gdp To Be Published.