Magnesium in PDB 7tid: Structure of the Yeast Clamp Loader (Replication Factor C Rfc) Bound to the Sliding Clamp (Proliferating Cell Nuclear Antigen Pcna) and Primer-Template Dna

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Structure of the Yeast Clamp Loader (Replication Factor C Rfc) Bound to the Sliding Clamp (Proliferating Cell Nuclear Antigen Pcna) and Primer-Template Dna (pdb code 7tid). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Structure of the Yeast Clamp Loader (Replication Factor C Rfc) Bound to the Sliding Clamp (Proliferating Cell Nuclear Antigen Pcna) and Primer-Template Dna, PDB code: 7tid:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in 7tid

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Magnesium binding site 1 out of 4 in the Structure of the Yeast Clamp Loader (Replication Factor C Rfc) Bound to the Sliding Clamp (Proliferating Cell Nuclear Antigen Pcna) and Primer-Template Dna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of the Yeast Clamp Loader (Replication Factor C Rfc) Bound to the Sliding Clamp (Proliferating Cell Nuclear Antigen Pcna) and Primer-Template Dna within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg902

b:55.1
occ:1.00
O3G A:AGS901 2.1 64.7 1.0
OG1 A:THR360 2.4 66.3 1.0
O1B A:AGS901 2.4 64.7 1.0
S1G A:AGS901 2.7 64.7 1.0
PG A:AGS901 2.8 64.7 1.0
CB A:THR360 3.3 66.3 1.0
PB A:AGS901 3.5 64.7 1.0
O3B A:AGS901 3.6 64.7 1.0
OD2 A:ASP424 3.6 68.3 1.0
O1A A:AGS901 3.8 64.7 1.0
CG2 A:THR360 4.1 66.3 1.0
O2G A:AGS901 4.2 64.7 1.0
O3A A:AGS901 4.2 64.7 1.0
PA A:AGS901 4.4 64.7 1.0
CG A:ASP424 4.5 68.3 1.0
N A:THR360 4.5 66.3 1.0
CA A:THR360 4.5 66.3 1.0
O2A A:AGS901 4.5 64.7 1.0
NH1 B:ARG129 4.6 54.1 1.0
NH2 B:ARG128 4.6 51.2 1.0
NH2 B:ARG157 4.7 51.1 1.0
CG2 A:ILE454 4.8 67.5 1.0
O2B A:AGS901 4.8 64.7 1.0

Magnesium binding site 2 out of 4 in 7tid

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Magnesium binding site 2 out of 4 in the Structure of the Yeast Clamp Loader (Replication Factor C Rfc) Bound to the Sliding Clamp (Proliferating Cell Nuclear Antigen Pcna) and Primer-Template Dna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of the Yeast Clamp Loader (Replication Factor C Rfc) Bound to the Sliding Clamp (Proliferating Cell Nuclear Antigen Pcna) and Primer-Template Dna within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg402

b:35.1
occ:1.00
O3G B:AGS401 2.1 40.3 1.0
OG1 B:THR56 2.1 35.9 1.0
CB B:THR56 3.2 35.9 1.0
O1B B:AGS401 3.2 40.3 1.0
PG B:AGS401 3.6 40.3 1.0
O1A B:AGS401 3.7 40.3 1.0
OE1 C:GLU135 3.8 36.8 1.0
CG2 B:THR56 3.8 35.9 1.0
NH2 C:ARG160 4.0 29.4 1.0
OE1 B:GLU115 4.1 37.4 1.0
S1G B:AGS401 4.1 40.3 1.0
PB B:AGS401 4.3 40.3 1.0
NE C:ARG132 4.4 35.7 1.0
CA B:THR56 4.4 35.9 1.0
NH2 B:ARG203 4.4 40.3 1.0
O3B B:AGS401 4.4 40.3 1.0
N B:THR56 4.5 35.9 1.0
OD1 B:ASP114 4.5 36.1 1.0
O2G B:AGS401 4.5 40.3 1.0
PA B:AGS401 4.6 40.3 1.0
OE2 C:GLU135 4.6 36.8 1.0
CD C:GLU135 4.6 36.8 1.0
O3A B:AGS401 4.7 40.3 1.0
CD C:ARG132 4.7 35.7 1.0
CG B:ASP114 4.8 36.1 1.0
O2A B:AGS401 4.9 40.3 1.0

Magnesium binding site 3 out of 4 in 7tid

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Magnesium binding site 3 out of 4 in the Structure of the Yeast Clamp Loader (Replication Factor C Rfc) Bound to the Sliding Clamp (Proliferating Cell Nuclear Antigen Pcna) and Primer-Template Dna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Structure of the Yeast Clamp Loader (Replication Factor C Rfc) Bound to the Sliding Clamp (Proliferating Cell Nuclear Antigen Pcna) and Primer-Template Dna within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Mg402

b:34.0
occ:1.00
O3G C:AGS401 2.1 48.5 1.0
OG1 C:THR60 2.1 31.2 1.0
O1B C:AGS401 2.8 48.5 1.0
CB C:THR60 3.3 31.2 1.0
PG C:AGS401 3.4 48.5 1.0
OD2 C:ASP117 3.4 28.1 1.0
NH2 D:ARG183 3.6 21.9 1.0
OD1 C:ASP117 3.7 28.1 1.0
S1G C:AGS401 3.7 48.5 1.0
CG C:ASP117 3.9 28.1 1.0
PB C:AGS401 4.0 48.5 1.0
O1A C:AGS401 4.0 48.5 1.0
CG2 C:THR60 4.0 31.2 1.0
O3B C:AGS401 4.1 48.5 1.0
OE2 C:GLU118 4.2 29.8 1.0
NH2 C:ARG206 4.3 29.1 1.0
CA C:THR60 4.5 31.2 1.0
N C:THR60 4.5 31.2 1.0
O2G C:AGS401 4.5 48.5 1.0
O3A C:AGS401 4.6 48.5 1.0
CD2 C:LEU146 4.7 25.0 1.0
PA C:AGS401 4.7 48.5 1.0
CG C:GLU118 4.7 29.8 1.0
NH1 D:ARG155 4.7 29.9 1.0
CZ D:ARG183 4.8 21.9 1.0
CD D:ARG155 4.8 29.9 1.0
O2A C:AGS401 4.9 48.5 1.0
CD C:GLU118 4.9 29.8 1.0

Magnesium binding site 4 out of 4 in 7tid

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Magnesium binding site 4 out of 4 in the Structure of the Yeast Clamp Loader (Replication Factor C Rfc) Bound to the Sliding Clamp (Proliferating Cell Nuclear Antigen Pcna) and Primer-Template Dna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Structure of the Yeast Clamp Loader (Replication Factor C Rfc) Bound to the Sliding Clamp (Proliferating Cell Nuclear Antigen Pcna) and Primer-Template Dna within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Mg402

b:34.1
occ:1.00
O3G D:AGS401 2.2 48.5 1.0
OG1 D:THR72 2.3 29.4 1.0
O2B D:AGS401 2.4 48.5 1.0
OD1 D:ASP140 3.4 29.0 1.0
PG D:AGS401 3.4 48.5 1.0
CB D:THR72 3.5 29.4 1.0
OD2 D:ASP140 3.6 29.0 1.0
S1G D:AGS401 3.8 48.5 1.0
NH2 E:ARG184 3.8 30.1 1.0
PB D:AGS401 3.8 48.5 1.0
CG D:ASP140 3.9 29.0 1.0
OE2 D:GLU141 4.0 30.0 1.0
O2A D:AGS401 4.0 48.5 1.0
O3B D:AGS401 4.1 48.5 1.0
CG2 D:THR72 4.4 29.4 1.0
N D:THR72 4.4 29.4 1.0
CA D:THR72 4.5 29.4 1.0
CG2 D:ILE169 4.6 20.8 1.0
O2G D:AGS401 4.6 48.5 1.0
O1B D:AGS401 4.6 48.5 1.0
OE1 E:GLU159 4.7 39.9 1.0
PA D:AGS401 4.8 48.5 1.0
O3A D:AGS401 4.8 48.5 1.0
O1A D:AGS401 4.8 48.5 1.0
CZ E:ARG184 4.9 30.1 1.0
CD D:GLU141 4.9 30.0 1.0

Reference:

C.Gaubitz, X.Liu, J.Pajak, N.P.Stone, J.A.Hayes, G.Demo, B.A.Kelch Phd. Cryo-Em Structures Reveal High-Resolution Mechanism of A Dna Polymerase Sliding Clamp Loader. Elife V. 11 2022.
ISSN: ESSN 2050-084X
PubMed: 35179493
DOI: 10.7554/ELIFE.74175
Page generated: Thu Apr 6 22:56:44 2023

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