Magnesium in PDB 7vw5: Crystal Structures of Alphavirus Nonstructural Protein 4 (NSP4) Reveal An Intrinsically Dynamic Rna-Dependent Rna Polymerase Fold

All present enzymatic activity of Crystal Structures of Alphavirus Nonstructural Protein 4 (NSP4) Reveal An Intrinsically Dynamic Rna-Dependent Rna Polymerase Fold:
2.7.7.48;

The structure of Crystal Structures of Alphavirus Nonstructural Protein 4 (NSP4) Reveal An Intrinsically Dynamic Rna-Dependent Rna Polymerase Fold, PDB code: 7vw5 was solved by Y.B.Tan, D.Luo, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.27 / 2.30
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	67.45, 68.94, 71.42, 116.31, 107.05, 95.1
R / Rfree (%)	19.7 / 24.1

The binding sites of Magnesium atom in the Crystal Structures of Alphavirus Nonstructural Protein 4 (NSP4) Reveal An Intrinsically Dynamic Rna-Dependent Rna Polymerase Fold (pdb code 7vw5). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structures of Alphavirus Nonstructural Protein 4 (NSP4) Reveal An Intrinsically Dynamic Rna-Dependent Rna Polymerase Fold, PDB code: 7vw5:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structures of Alphavirus Nonstructural Protein 4 (NSP4) Reveal An Intrinsically Dynamic Rna-Dependent Rna Polymerase Fold


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structures of Alphavirus Nonstructural Protein 4 (NSP4) Reveal An Intrinsically Dynamic Rna-Dependent Rna Polymerase Fold within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg701 b:30.5 occ:0.00 O A:HOH842 2.0 32.6 1.0 O A:HOH885 2.0 37.1 1.0 O A:HOH829 2.0 30.8 1.0 O A:HOH867 2.0 33.3 1.0 O A:HOH955 2.1 31.7 1.0 OD1 A:ASP392 2.1 26.1 1.0 CG A:ASP392 3.0 28.9 1.0 OD2 A:ASP392 3.3 31.6 1.0 O A:MET388 4.1 23.8 1.0 OE1 A:GLU391 4.2 38.2 1.0 NH2 A:ARG228 4.4 29.4 1.0 CB A:ASP392 4.4 21.9 1.0 O A:HOH891 4.5 26.7 1.0 CA A:ASP392 4.7 26.3 1.0 N A:ASP392 4.8 25.4 1.0 CB A:MET388 4.8 21.6 1.0 CD A:GLU391 4.8 41.7 1.0 C A:MET388 4.8 27.8 1.0 CG A:GLU391 5.0 38.8 1.0

Magnesium binding site 2 out of 2 in the Crystal Structures of Alphavirus Nonstructural Protein 4 (NSP4) Reveal An Intrinsically Dynamic Rna-Dependent Rna Polymerase Fold


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structures of Alphavirus Nonstructural Protein 4 (NSP4) Reveal An Intrinsically Dynamic Rna-Dependent Rna Polymerase Fold within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg701 b:27.0 occ:0.00 OD1 B:ASP392 1.9 22.9 1.0 O B:HOH922 2.0 31.5 1.0 O B:HOH942 2.0 27.0 1.0 O B:HOH960 2.0 24.9 1.0 O B:HOH810 2.0 25.2 1.0 O B:HOH826 2.0 28.2 1.0 CG B:ASP392 2.8 24.9 1.0 OD2 B:ASP392 3.1 25.9 1.0 NH2 B:ARG228 4.0 29.4 1.0 O B:HOH865 4.1 21.3 1.0 O B:HOH962 4.1 45.6 1.0 O B:MET388 4.1 22.6 1.0 CB B:ASP392 4.2 21.1 1.0 OE1 B:GLU391 4.4 39.8 1.0 CA B:ASP392 4.6 24.9 1.0 O B:HOH955 4.8 31.4 1.0 N B:ASP392 4.8 20.7 1.0 CD B:GLU391 4.9 44.3 1.0 CG B:GLU391 5.0 34.3 1.0 C B:MET388 5.0 24.3 1.0

Y.B.Tan, L.S.Lello, X.Liu, Y.S.Law, C.Kang, J.Lescar, J.Zheng, A.Merits, D.Luo. Crystal Structures of Alphavirus Nonstructural Protein 4 (NSP4) Reveal An Intrinsically Dynamic Rna-Dependent Rna Polymerase Fold. Nucleic Acids Res. V. 50 1000 2022.
ISSN: ESSN 1362-4962
PubMed: 35037043
DOI: 10.1093/NAR/GKAB1302