Magnesium in PDB 7wz0: Cryo-Em Structure of Na+,K+-Atpase in the E2P State Formed By Inorganic Phosphate with Ouabain

The binding sites of Magnesium atom in the Cryo-Em Structure of Na+,K+-Atpase in the E2P State Formed By Inorganic Phosphate with Ouabain (pdb code 7wz0). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 6 binding sites of Magnesium where determined in the Cryo-Em Structure of Na+,K+-Atpase in the E2P State Formed By Inorganic Phosphate with Ouabain, PDB code: 7wz0:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6;

Magnesium binding site 1 out of 6 in the Cryo-Em Structure of Na+,K+-Atpase in the E2P State Formed By Inorganic Phosphate with Ouabain


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of Na+,K+-Atpase in the E2P State Formed By Inorganic Phosphate with Ouabain within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1101 b:44.8 occ:1.00 OP3 A:PHD376 1.8 44.1 1.0 O A:HOH1204 2.0 44.5 1.0 O A:THR378 2.0 44.3 1.0 OD1 A:ASP717 2.1 47.3 1.0 O A:HOH1205 2.2 49.1 1.0 OD2 A:PHD376 2.2 44.1 1.0 CG A:ASP717 3.0 47.3 1.0 P A:PHD376 3.2 44.1 1.0 C A:THR378 3.2 44.3 1.0 OD2 A:ASP717 3.2 47.3 1.0 CG A:PHD376 3.2 44.1 1.0 OD1 A:PHD376 3.5 44.1 1.0 OD2 A:ASP721 3.9 48.7 1.0 CB A:THR378 3.9 44.3 1.0 OP1 A:PHD376 4.0 44.1 1.0 CA A:THR378 4.0 44.3 1.0 N A:GLY718 4.0 49.5 1.0 N A:GLY379 4.2 41.6 1.0 OP2 A:PHD376 4.2 44.1 1.0 CG2 A:THR378 4.2 44.3 1.0 N A:THR378 4.3 44.3 1.0 CB A:ASP717 4.4 47.3 1.0 CA A:GLY379 4.4 41.6 1.0 OD1 A:ASN720 4.5 51.8 1.0 CB A:PHD376 4.5 44.1 1.0 N A:ASP717 4.6 47.3 1.0 OG1 A:THR380 4.6 41.3 1.0 CA A:GLY718 4.7 49.5 1.0 O A:SER216 4.7 68.0 1.0 CA A:ASP717 4.8 47.3 1.0 ND2 A:ASN720 4.8 51.8 1.0 C A:ASP717 4.9 47.3 1.0 N A:THR380 4.9 41.3 1.0 C A:GLY379 5.0 41.6 1.0 CG A:ASP721 5.0 48.7 1.0

Magnesium binding site 2 out of 6 in the Cryo-Em Structure of Na+,K+-Atpase in the E2P State Formed By Inorganic Phosphate with Ouabain


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Cryo-Em Structure of Na+,K+-Atpase in the E2P State Formed By Inorganic Phosphate with Ouabain within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1102 b:47.5 occ:1.00 OD2 A:ASP747 2.1 38.8 1.0 CG A:ASP747 3.3 38.8 1.0 O A:LYS726 3.6 43.2 1.0 OD1 A:ASP747 3.9 38.8 1.0 O A:ALA728 4.1 39.6 1.0 O A:LEU725 4.3 39.8 1.0 O A:VAL279 4.3 62.8 1.0 CB A:ASP747 4.4 38.8 1.0 C A:LYS726 4.5 43.2 1.0 CA A:LYS726 4.7 43.2 1.0 O A:ASP729 4.8 39.4 1.0 CD A:LYS726 4.9 43.2 1.0 O A:ALA745 5.0 43.5 1.0

Magnesium binding site 3 out of 6 in the Cryo-Em Structure of Na+,K+-Atpase in the E2P State Formed By Inorganic Phosphate with Ouabain


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Cryo-Em Structure of Na+,K+-Atpase in the E2P State Formed By Inorganic Phosphate with Ouabain within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1103 b:32.5 occ:1.00 O A:HOH1203 2.1 33.5 1.0 OD2 A:ASP811 2.2 32.3 1.0 O A:HOH1202 2.3 29.4 1.0 OD1 A:ASP811 2.3 32.3 1.0 O A:HOH1201 2.4 34.8 1.0 CG A:ASP811 2.5 32.3 1.0 OE1 A:GLU786 2.7 25.5 1.0 OE2 A:GLU334 2.8 37.3 1.0 CD A:GLU786 3.7 25.5 1.0 CB A:ASP811 3.9 32.3 1.0 CD A:GLU334 3.9 37.3 1.0 OE2 A:GLU786 4.0 25.5 1.0 OE1 A:GLU334 4.4 37.3 1.0 O A:VAL332 4.4 31.6 1.0 O A:ALA330 4.5 28.6 1.0 OD1 A:ASN783 4.6 27.5 1.0 CG2 A:ILE807 4.7 27.8 1.0

Magnesium binding site 4 out of 6 in the Cryo-Em Structure of Na+,K+-Atpase in the E2P State Formed By Inorganic Phosphate with Ouabain


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Cryo-Em Structure of Na+,K+-Atpase in the E2P State Formed By Inorganic Phosphate with Ouabain within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg1101 b:45.5 occ:1.00 OP3 C:PHD376 1.8 44.4 1.0 O C:HOH1204 2.0 45.1 1.0 O C:THR378 2.0 44.5 1.0 OD1 C:ASP717 2.1 47.8 1.0 O C:HOH1205 2.2 49.2 1.0 OD2 C:PHD376 2.2 44.4 1.0 CG C:ASP717 3.0 47.8 1.0 P C:PHD376 3.2 44.4 1.0 C C:THR378 3.2 44.5 1.0 OD2 C:ASP717 3.2 47.8 1.0 CG C:PHD376 3.2 44.4 1.0 OD1 C:PHD376 3.5 44.4 1.0 OD2 C:ASP721 3.9 48.7 1.0 CB C:THR378 3.9 44.5 1.0 OP1 C:PHD376 4.0 44.4 1.0 CA C:THR378 4.0 44.5 1.0 N C:GLY718 4.0 49.9 1.0 N C:GLY379 4.2 42.2 1.0 OP2 C:PHD376 4.2 44.4 1.0 CG2 C:THR378 4.2 44.5 1.0 N C:THR378 4.3 44.5 1.0 CB C:ASP717 4.4 47.8 1.0 CA C:GLY379 4.4 42.2 1.0 OD1 C:ASN720 4.5 52.0 1.0 CB C:PHD376 4.5 44.4 1.0 N C:ASP717 4.6 47.8 1.0 OG1 C:THR380 4.6 41.5 1.0 CA C:GLY718 4.7 49.9 1.0 O C:SER216 4.7 68.3 1.0 CA C:ASP717 4.8 47.8 1.0 ND2 C:ASN720 4.8 52.0 1.0 C C:ASP717 4.9 47.8 1.0 N C:THR380 4.9 41.5 1.0 C C:GLY379 5.0 42.2 1.0 CG C:ASP721 5.0 48.7 1.0

Magnesium binding site 5 out of 6 in the Cryo-Em Structure of Na+,K+-Atpase in the E2P State Formed By Inorganic Phosphate with Ouabain


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Cryo-Em Structure of Na+,K+-Atpase in the E2P State Formed By Inorganic Phosphate with Ouabain within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg1102 b:48.6 occ:1.00 OD2 C:ASP747 2.1 39.2 1.0 CG C:ASP747 3.3 39.2 1.0 O C:LYS726 3.6 43.6 1.0 OD1 C:ASP747 3.9 39.2 1.0 O C:ALA728 4.1 39.8 1.0 O C:LEU725 4.3 40.3 1.0 O C:VAL279 4.3 62.1 1.0 CB C:ASP747 4.4 39.2 1.0 C C:LYS726 4.5 43.6 1.0 CA C:LYS726 4.7 43.6 1.0 O C:ASP729 4.8 39.5 1.0 CD C:LYS726 4.9 43.6 1.0 O C:ALA745 5.0 43.8 1.0

Magnesium binding site 6 out of 6 in the Cryo-Em Structure of Na+,K+-Atpase in the E2P State Formed By Inorganic Phosphate with Ouabain


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of Cryo-Em Structure of Na+,K+-Atpase in the E2P State Formed By Inorganic Phosphate with Ouabain within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg1103 b:33.4 occ:1.00 O C:HOH1203 2.1 34.7 1.0 OD2 C:ASP811 2.2 32.4 1.0 O C:HOH1202 2.3 29.9 1.0 OD1 C:ASP811 2.3 32.4 1.0 O C:HOH1201 2.4 34.7 1.0 CG C:ASP811 2.5 32.4 1.0 OE1 C:GLU786 2.7 25.4 1.0 OE2 C:GLU334 2.8 37.7 1.0 CD C:GLU786 3.7 25.4 1.0 CB C:ASP811 3.9 32.4 1.0 CD C:GLU334 3.9 37.7 1.0 OE2 C:GLU786 4.0 25.4 1.0 OE1 C:GLU334 4.4 37.7 1.0 O C:VAL332 4.4 31.5 1.0 O C:ALA330 4.5 28.6 1.0 OD1 C:ASN783 4.6 27.6 1.0 CG2 C:ILE807 4.7 28.0 1.0

R.Kanai, F.Cornelius, B.Vilsen, C.Toyoshima. Cryoelectron Microscopy of Na + ,K + -Atpase in the Two E2P States with and Without Cardiotonic Steroids. Proc.Natl.Acad.Sci.Usa V. 119 26119 2022.
ISSN: ESSN 1091-6490
PubMed: 35380894
DOI: 10.1073/PNAS.2123226119