Magnesium in PDB 7xug: Cryo-Em Structure of HK022 Putrna-Less E.Coli Rna Polymerase Elongation Complex

All present enzymatic activity of Cryo-Em Structure of HK022 Putrna-Less E.Coli Rna Polymerase Elongation Complex:
2.7.7.6;

The structure of Cryo-Em Structure of HK022 Putrna-Less E.Coli Rna Polymerase Elongation Complex also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Magnesium atom in the Cryo-Em Structure of HK022 Putrna-Less E.Coli Rna Polymerase Elongation Complex (pdb code 7xug). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Cryo-Em Structure of HK022 Putrna-Less E.Coli Rna Polymerase Elongation Complex, PDB code: 7xug:

Magnesium binding site 1 out of 1 in the Cryo-Em Structure of HK022 Putrna-Less E.Coli Rna Polymerase Elongation Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of HK022 Putrna-Less E.Coli Rna Polymerase Elongation Complex within 5.0Å range: probe atom residue distance (Å) B Occ J:Mg1503 b:23.4 occ:1.00 OD1 J:ASP462 1.9 30.5 1.0 O3' R:C94 2.2 51.4 1.0 OD1 J:ASP460 2.4 32.2 1.0 OD1 J:ASP464 2.5 40.6 1.0 CG J:ASP462 2.7 30.5 1.0 OD2 J:ASP462 2.8 30.5 1.0 OD2 J:ASP464 3.1 40.6 1.0 CG J:ASP464 3.2 40.6 1.0 CG J:ASP460 3.5 32.2 1.0 C3' R:C94 3.6 51.4 1.0 OD2 J:ASP460 3.8 32.2 1.0 CB J:ASP462 4.2 30.5 1.0 C4' R:C94 4.2 51.4 1.0 O J:ASP460 4.4 32.2 1.0 O2' R:C94 4.5 51.4 1.0 C5' R:C94 4.5 51.4 1.0 N J:ASP462 4.5 30.5 1.0 C2' R:C94 4.6 51.4 1.0 CB J:ASP464 4.6 40.6 1.0 N J:ASP460 4.6 32.2 1.0 C J:ASP460 4.7 32.2 1.0 CA J:ASP462 4.8 30.5 1.0 CB J:ASP460 4.8 32.2 1.0 CA J:ASP460 5.0 32.2 1.0 C J:ASP462 5.0 30.5 1.0

S.Hwang, P.D.B.Olinares, J.Lee, J.Kim, B.T.Chait, R.A.King, J.Y.Kang. Structural Basis of Transcriptional Regulation By A Nascent Rna Element, HK022 Putrna. Nat Commun V. 13 4668 2022.
ISSN: ESSN 2041-1723
PubMed: 35970830
DOI: 10.1038/S41467-022-32315-Y