Magnesium in PDB 7z01: Z-SBTUBA4 Photoswitch Bound to Tubulin-Darpin D1 Complex

The structure of Z-SBTUBA4 Photoswitch Bound to Tubulin-Darpin D1 Complex, PDB code: 7z01 was solved by M.Wranik, T.Weinert, J.Standfuss, M.Steinmetz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.19 / 1.82
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	73.468, 91.891, 82.434, 90, 97.4, 90
R / Rfree (%)	20.5 / 25.5

The structure of Z-SBTUBA4 Photoswitch Bound to Tubulin-Darpin D1 Complex also contains other interesting chemical elements:

Calcium

(Ca)

1 atom

The binding sites of Magnesium atom in the Z-SBTUBA4 Photoswitch Bound to Tubulin-Darpin D1 Complex (pdb code 7z01). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Z-SBTUBA4 Photoswitch Bound to Tubulin-Darpin D1 Complex, PDB code: 7z01:

Magnesium binding site 1 out of 1 in the Z-SBTUBA4 Photoswitch Bound to Tubulin-Darpin D1 Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Z-SBTUBA4 Photoswitch Bound to Tubulin-Darpin D1 Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg502 b:26.6 occ:1.00 O A:HOH663 2.0 32.0 1.0 O2B A:GTP501 2.0 29.0 1.0 O1G A:GTP501 2.3 27.5 1.0 O A:HOH640 2.4 26.0 1.0 PB A:GTP501 3.3 24.8 1.0 PG A:GTP501 3.5 31.8 1.0 OE2 A:GLU71 3.7 42.1 1.0 O3B A:GTP501 3.8 32.7 1.0 O3A A:GTP501 3.9 29.0 1.0 CB A:GLN11 3.9 21.7 1.0 NZ B:LYS254 4.1 46.0 1.0 O2G A:GTP501 4.2 26.9 1.0 OD2 A:ASP69 4.3 25.3 1.0 CG A:GLU71 4.3 31.2 1.0 OD1 A:ASP69 4.3 24.8 1.0 OE1 A:GLN11 4.4 28.4 1.0 N A:GLN11 4.4 16.3 1.0 CD A:GLU71 4.4 46.2 1.0 O1B A:GTP501 4.5 21.7 1.0 O3G A:GTP501 4.7 17.8 1.0 O2A A:GTP501 4.7 20.9 1.0 CG A:ASP69 4.8 25.8 1.0 O A:HOH634 4.8 35.2 1.0 CB A:ASP98 4.8 24.9 1.0 CA A:GLN11 4.8 20.3 1.0 PA A:GTP501 4.8 26.2 1.0 CD A:GLN11 4.9 20.9 1.0

L.Gao, J.C.M.Meiring, A.Varady, I.E.Ruider, C.Heise, M.Wranik, C.D.Velasco, J.A.Taylor, B.Terni, T.Weinert, J.Standfuss, C.C.Cabernard, A.Llobet, M.O.Steinmetz, A.R.Bausch, M.Distel, J.Thorn-Seshold, A.Akhmanova, O.Thorn-Seshold. In Vivo Photocontrol of Microtubule Dynamics and Integrity, Migration and Mitosis, By the Potent Gfp-Imaging-Compatible Photoswitchable Reagents SBTUBA4P and SBTUB2M. J.Am.Chem.Soc. V. 144 5614 2022.
ISSN: ESSN 1520-5126
PubMed: 35290733
DOI: 10.1021/JACS.2C01020