Magnesium in PDB 7z1j: Escherichia Coli Periplasmic Phytase Appa, Complex with Phosphate

All present enzymatic activity of Escherichia Coli Periplasmic Phytase Appa, Complex with Phosphate:
3.1.3.2; 3.1.3.26;

The structure of Escherichia Coli Periplasmic Phytase Appa, Complex with Phosphate, PDB code: 7z1j was solved by I.M.Acquistapace, C.A.Brearley, A.M.Hemmings, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.85 / 1.85
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	63.57, 47.63, 65.7, 90, 101, 90
R / Rfree (%)	14.8 / 22.7

The structure of Escherichia Coli Periplasmic Phytase Appa, Complex with Phosphate also contains other interesting chemical elements:

Nickel

(Ni)

1 atom

The binding sites of Magnesium atom in the Escherichia Coli Periplasmic Phytase Appa, Complex with Phosphate (pdb code 7z1j). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Escherichia Coli Periplasmic Phytase Appa, Complex with Phosphate, PDB code: 7z1j:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Escherichia Coli Periplasmic Phytase Appa, Complex with Phosphate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Escherichia Coli Periplasmic Phytase Appa, Complex with Phosphate within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg504 b:29.0 occ:1.00 O A:HOH989 2.0 55.3 1.0 NE2 A:HIS113 2.1 19.2 1.0 O A:HOH760 2.3 39.6 1.0 O A:HOH758 2.3 43.4 1.0 O A:HOH958 2.5 41.8 1.0 O A:HOH884 2.7 49.7 1.0 CD2 A:HIS113 2.9 16.3 1.0 CE1 A:HIS113 3.3 19.0 1.0 CG A:HIS113 4.1 16.0 1.0 O A:GLY290 4.1 26.4 1.0 ND1 A:HIS113 4.3 16.8 1.0 O A:HOH1026 4.4 23.7 1.0 O A:TYR289 4.4 25.0 1.0 C A:GLY290 4.7 24.8 1.0 CG2 A:THR111 4.9 16.1 1.0

Magnesium binding site 2 out of 2 in the Escherichia Coli Periplasmic Phytase Appa, Complex with Phosphate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Escherichia Coli Periplasmic Phytase Appa, Complex with Phosphate within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg505 b:24.5 occ:0.80 O A:HOH759 2.0 37.0 1.0 O A:HOH1063 2.0 32.7 1.0 OD1 A:ASP69 2.0 20.2 1.0 O A:HOH624 2.2 28.7 1.0 O A:HOH934 2.3 34.6 1.0 O A:HOH982 2.5 52.8 1.0 CG A:ASP69 2.9 20.0 1.0 OD2 A:ASP69 3.2 21.9 1.0 O A:HOH815 3.9 31.7 1.0 CB A:ASP69 4.3 18.1 1.0 O A:HOH1051 4.4 32.9 1.0 O A:ARG65 4.6 10.8 1.0 CA A:ASP69 4.7 15.8 1.0 N A:ASP69 4.7 15.1 1.0

I.M.Acquistapace, E.J.Thompson, I.Kuhn, M.R.Bedford, C.A.Brearley, A.M.Hemmings. Insights to the Structural Basis For the Stereospecificity of the Escherichia Coli Phytase, Appa. Int J Mol Sci V. 23 2022.
ISSN: ESSN 1422-0067
PubMed: 35683026
DOI: 10.3390/IJMS23116346