Magnesium in PDB 8a3v: Crystal Structure of the Vibrio Cholerae Replicative Helicase (Vcdnab) in Complex with Its Loader Protein (Vcdcia)

All present enzymatic activity of Crystal Structure of the Vibrio Cholerae Replicative Helicase (Vcdnab) in Complex with Its Loader Protein (Vcdcia):
3.6.4.12;

The structure of Crystal Structure of the Vibrio Cholerae Replicative Helicase (Vcdnab) in Complex with Its Loader Protein (Vcdcia), PDB code: 8a3v was solved by H.Walbott, S.Quevillon-Cheruel, C.Cargemel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.80 / 2.90
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	186.515, 186.515, 252.839, 90, 90, 120
R / Rfree (%)	27.9 / 29

The binding sites of Magnesium atom in the Crystal Structure of the Vibrio Cholerae Replicative Helicase (Vcdnab) in Complex with Its Loader Protein (Vcdcia) (pdb code 8a3v). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Vibrio Cholerae Replicative Helicase (Vcdnab) in Complex with Its Loader Protein (Vcdcia), PDB code: 8a3v:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Vibrio Cholerae Replicative Helicase (Vcdnab) in Complex with Its Loader Protein (Vcdcia)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Vibrio Cholerae Replicative Helicase (Vcdnab) in Complex with Its Loader Protein (Vcdcia) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg502 b:63.7 occ:1.00 OG1 A:THR235 2.1 97.1 1.0 OD1 A:ASP340 2.8 228.1 1.0 CE2 A:TYR341 2.8 225.6 1.0 OE2 A:GLU259 3.1 120.5 1.0 OD2 A:ASP340 3.1 229.7 1.0 CG A:ASP340 3.3 227.6 1.0 CB A:THR235 3.3 96.1 1.0 CG2 A:THR235 3.6 96.5 1.0 CE A:MET260 3.6 113.6 1.0 OH A:TYR341 3.7 228.0 1.0 CZ A:TYR341 3.7 226.5 1.0 CD2 A:TYR341 3.7 224.6 1.0 O1B A:ADP501 4.0 72.2 1.0 CD A:GLU259 4.2 118.8 1.0 SD A:MET260 4.2 115.3 1.0 CA A:THR235 4.5 94.7 1.0 CG A:MET260 4.6 111.4 1.0 CB A:GLU259 4.6 110.0 1.0 CD1 A:LEU379 4.7 179.3 1.0 CB A:ASP340 4.8 223.4 1.0 N A:THR235 4.9 94.7 1.0 O A:ASP340 5.0 222.3 1.0 OE1 A:GLU259 5.0 119.5 1.0 CE1 A:TYR341 5.0 225.5 1.0 CG A:GLU259 5.0 113.9 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Vibrio Cholerae Replicative Helicase (Vcdnab) in Complex with Its Loader Protein (Vcdcia)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Vibrio Cholerae Replicative Helicase (Vcdnab) in Complex with Its Loader Protein (Vcdcia) within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg502 b:73.7 occ:1.00 OG1 B:THR235 2.1 86.5 1.0 OD1 B:ASP340 2.8 131.6 1.0 CE2 B:TYR341 2.8 129.2 1.0 OE2 B:GLU259 3.0 79.9 1.0 OD2 B:ASP340 3.1 132.9 1.0 CB B:THR235 3.3 85.5 1.0 CG B:ASP340 3.3 131.0 1.0 CG2 B:THR235 3.5 86.0 1.0 CE B:MET260 3.6 77.8 1.0 OH B:TYR341 3.7 131.6 1.0 CZ B:TYR341 3.7 130.1 1.0 CD2 B:TYR341 3.7 128.3 1.0 O1B B:ADP501 4.0 194.8 1.0 CD B:GLU259 4.2 78.5 1.0 SD B:MET260 4.2 78.6 1.0 CA B:THR235 4.5 84.1 1.0 CG B:MET260 4.6 72.0 1.0 CB B:GLU259 4.6 71.2 1.0 CD1 B:LEU379 4.7 134.3 1.0 CB B:ASP340 4.8 127.2 1.0 N B:THR235 4.9 84.0 1.0 O B:ASP340 5.0 126.5 1.0 OE1 B:GLU259 5.0 79.5 1.0 CE1 B:TYR341 5.0 129.2 1.0 CG B:GLU259 5.0 74.3 1.0

C.Cargemel, S.Marsin, M.Noiray, P.Legrand, H.Bounoua, I.Li De La Sierra-Gallay, H.Walbott, S.Quevillon-Cheruel. The Lh-Dh Module of Bacterial Replicative Helicases Is the Common Binding Site For Dcia and Other Helicase Loaders Acta Crystallogr.,Sect.D 2023.
ISSN: ESSN 1399-0047
DOI: 10.1107/S2059798323000281