Magnesium in PDB 8aan: The Nucleoprotein Complex of Rep Protein with Iteron Containing Dsdna and Due Ssdna

The structure of The Nucleoprotein Complex of Rep Protein with Iteron Containing Dsdna and Due Ssdna, PDB code: 8aan was solved by M.Nowacka, K.Wegrzyn, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.94 / 2.19
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	109.313, 81.047, 75.833, 90, 123.52, 90
R / Rfree (%)	18.7 / 23.3

The binding sites of Magnesium atom in the The Nucleoprotein Complex of Rep Protein with Iteron Containing Dsdna and Due Ssdna (pdb code 8aan). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the The Nucleoprotein Complex of Rep Protein with Iteron Containing Dsdna and Due Ssdna, PDB code: 8aan:

Magnesium binding site 1 out of 1 in the The Nucleoprotein Complex of Rep Protein with Iteron Containing Dsdna and Due Ssdna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of The Nucleoprotein Complex of Rep Protein with Iteron Containing Dsdna and Due Ssdna within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg301 b:26.4 occ:0.00 O A:HOH422 1.8 26.5 1.0 O A:HOH504 1.8 22.6 1.0 OD1 A:ASP81 1.9 20.7 1.0 OE1 A:GLU77 2.0 29.2 1.0 O A:HOH442 2.1 28.3 1.0 O A:HOH491 2.1 29.1 1.0 CG A:ASP81 3.0 25.2 1.0 CD A:GLU77 3.2 29.7 1.0 OD2 A:ASP81 3.4 28.1 1.0 O A:HOH462 3.5 27.3 1.0 CG A:GLU77 3.8 27.7 1.0 O A:HOH427 4.0 23.4 1.0 OD2 A:ASP34 4.2 24.2 1.0 OE2 A:GLU77 4.2 35.2 1.0 CB A:ASP81 4.3 22.9 1.0 OP2 B:DG14 4.5 30.5 1.0 CA A:ASP81 4.5 23.4 1.0 NH2 A:ARG33 4.5 26.2 1.0 OP2 B:DT13 4.6 28.3 1.0 N A:ASP81 4.6 22.5 1.0 O A:GLU77 4.6 32.2 1.0 CD A:LYS80 4.7 30.0 1.0 N7 B:DG14 4.9 30.5 1.0

K.Wegrzyn, M.Oliwa, M.Nowacka, E.Zabrocka, K.Bury, P.Purzycki, P.Czaplewska, J.Pipka, R.Giraldo, I.Konieczny. Rep Protein Accommodates Together Dsdna and Ssdna Which Enables A Loop-Back Mechanism to Plasmid Dna Replication Initiation. Nucleic Acids Res. 2023.
ISSN: ESSN 1362-4962
PubMed: 37713613
DOI: 10.1093/NAR/GKAD740