Magnesium in PDB 8alh: X-Ray Structure of Human Ncs-1 Bound to Ric-8A

The structure of X-Ray Structure of Human Ncs-1 Bound to Ric-8A, PDB code: 8alh was solved by D.Munoz-Reyes, M.J.Sanchez-Barrena, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	52.38 / 1.86
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	56.861, 56.861, 134.612, 90, 90, 90
R / Rfree (%)	19.6 / 23.1

The structure of X-Ray Structure of Human Ncs-1 Bound to Ric-8A also contains other interesting chemical elements:

Calcium	(Ca)	2 atoms
Sodium	(Na)	1 atom
Chlorine	(Cl)	1 atom

The binding sites of Magnesium atom in the X-Ray Structure of Human Ncs-1 Bound to Ric-8A (pdb code 8alh). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the X-Ray Structure of Human Ncs-1 Bound to Ric-8A, PDB code: 8alh:

Magnesium binding site 1 out of 1 in the X-Ray Structure of Human Ncs-1 Bound to Ric-8A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of X-Ray Structure of Human Ncs-1 Bound to Ric-8A within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg204 b:25.9 occ:0.50 OD2 B:ASP111 2.1 22.1 1.0 O B:HOH386 2.1 36.0 0.5 O B:HOH359 2.1 32.8 0.5 O B:HOH310 2.2 30.5 1.0 CG B:ASP111 3.2 20.9 1.0 HB2 B:ASP111 3.5 33.1 1.0 CB B:ASP111 3.7 27.6 1.0 HB3 B:ASP111 3.7 33.1 1.0 OD2 B:ASP113 3.9 28.7 1.0 HB3 B:ASP113 4.0 36.3 1.0 O B:HOH420 4.2 47.9 1.0 OD1 B:ASP111 4.3 25.5 1.0 O B:HOH372 4.3 25.0 1.0 O B:HOH333 4.4 38.0 1.0 O B:HOH357 4.5 33.3 1.0 CG B:ASP113 4.5 28.2 1.0 CB B:ASP113 4.7 30.2 1.0

D.Munoz-Reyes, L.J.Mcclelland, S.Arroyo-Urea, S.Sanchez-Yepes, J.Sabin, S.Perez-Suarez, M.Menendez, A.Mansilla, J.Garcia-Nafria, S.Sprang, M.J.Sanchez-Barrena. The Neuronal Calcium Sensor Ncs-1 Regulates the Phosphorylation State and Activity of the Ga Chaperone and Gef Ric-8A. Elife V. 12 2023.
ISSN: ESSN 2050-084X
PubMed: 38018500
DOI: 10.7554/ELIFE.86151