Magnesium in PDB 8asn: Crystal Structure of the Apo Human Ttl in Complex with Tubulin- Stathmin

All present enzymatic activity of Crystal Structure of the Apo Human Ttl in Complex with Tubulin- Stathmin:
6.3.2.25;

The structure of Crystal Structure of the Apo Human Ttl in Complex with Tubulin- Stathmin, PDB code: 8asn was solved by L.Vuillard, L.Miallau, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	133.66 / 2.57
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	82.553, 199.636, 134.669, 90, 97.02, 90
R / Rfree (%)	24.3 / 27.3

The binding sites of Magnesium atom in the Crystal Structure of the Apo Human Ttl in Complex with Tubulin- Stathmin (pdb code 8asn). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Crystal Structure of the Apo Human Ttl in Complex with Tubulin- Stathmin, PDB code: 8asn:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Crystal Structure of the Apo Human Ttl in Complex with Tubulin- Stathmin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Apo Human Ttl in Complex with Tubulin- Stathmin within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg501 b:42.8 occ:1.00 O2B A:GTP502 2.3 38.5 1.0 O1G A:GTP502 2.6 37.3 1.0 PB A:GTP502 3.5 38.2 1.0 OE2 A:GLU71 3.5 70.9 1.0 PG A:GTP502 3.6 36.9 1.0 O3B A:GTP502 3.8 37.5 1.0 O2G A:GTP502 3.8 37.2 1.0 CG A:GLU71 3.9 60.0 1.0 NZ B:LYS254 3.9 49.1 1.0 OD1 A:ASP69 4.0 53.6 1.0 O3A A:GTP502 4.1 38.9 1.0 CD A:GLU71 4.2 68.0 1.0 OD2 A:ASP69 4.2 54.3 1.0 CB A:GLN11 4.3 38.3 1.0 OD2 A:ASP98 4.4 53.3 1.0 CB A:ASP98 4.4 44.0 1.0 OE1 A:GLN11 4.5 51.1 1.0 CG A:ASP69 4.6 52.3 1.0 N A:GLN11 4.6 35.0 1.0 CG A:ASP98 4.7 50.4 1.0 O1B A:GTP502 4.8 37.9 1.0 O2A A:GTP502 4.9 39.9 1.0 O3G A:GTP502 4.9 36.5 1.0

Magnesium binding site 2 out of 4 in the Crystal Structure of the Apo Human Ttl in Complex with Tubulin- Stathmin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Apo Human Ttl in Complex with Tubulin- Stathmin within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg502 b:32.3 occ:1.00 O B:ALA9 2.5 37.8 1.0 N B:GLY13 2.9 38.3 1.0 N B:SER140 3.1 30.7 1.0 O2A B:GDP501 3.2 38.5 1.0 O2B B:GDP501 3.3 38.5 1.0 OG B:SER140 3.3 34.3 1.0 CA B:GLY146 3.4 33.0 1.0 CA B:HIS139 3.6 31.1 1.0 C B:ALA9 3.6 37.4 1.0 CA B:GLY13 3.6 38.6 1.0 CA B:GLY10 3.6 38.2 1.0 MG B:MG503 3.7 51.6 1.0 N B:CYS12 3.7 38.2 1.0 CB B:CYS12 3.7 37.8 1.0 C B:GLY10 3.8 38.6 1.0 C B:HIS139 3.8 30.9 1.0 C B:CYS12 3.9 38.5 1.0 CB B:HIS139 3.9 31.2 1.0 N B:GLY146 3.9 33.2 1.0 CA B:CYS12 3.9 38.0 1.0 CB B:SER140 4.0 32.2 1.0 N B:GLN11 4.0 38.7 1.0 N B:GLY10 4.1 37.6 1.0 CA B:SER140 4.1 31.1 1.0 O B:GLY10 4.2 38.8 1.0 O B:SER140 4.3 32.0 1.0 C B:GLY146 4.5 32.5 1.0 C B:GLN11 4.5 39.0 1.0 PA B:GDP501 4.5 38.5 1.0 PB B:GDP501 4.6 38.5 1.0 C B:SER140 4.7 31.6 1.0 O3A B:GDP501 4.8 38.4 1.0 N B:HIS139 4.8 31.8 1.0 SG B:CYS12 4.9 38.1 1.0 CA B:GLN11 4.9 39.7 1.0 CA B:ALA9 4.9 36.4 1.0 C B:GLY13 4.9 39.0 1.0 N B:SER147 5.0 31.9 1.0 O5' B:GDP501 5.0 37.9 1.0 O B:HIS139 5.0 30.6 1.0

Magnesium binding site 3 out of 4 in the Crystal Structure of the Apo Human Ttl in Complex with Tubulin- Stathmin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of the Apo Human Ttl in Complex with Tubulin- Stathmin within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg503 b:51.6 occ:1.00 O2B B:GDP501 2.5 38.5 1.0 N B:SER147 2.6 31.9 1.0 N B:GLY146 3.0 33.2 1.0 OG B:SER140 3.0 34.3 1.0 O B:SER140 3.1 32.0 1.0 CA B:GLY146 3.1 33.0 1.0 C B:GLY146 3.2 32.5 1.0 CA B:GLY143 3.3 32.5 1.0 C B:GLY143 3.4 32.2 1.0 N B:GLY144 3.5 31.5 1.0 N B:GLY143 3.5 32.6 1.0 CB B:SER147 3.5 33.2 1.0 PB B:GDP501 3.5 38.5 1.0 CA B:SER147 3.6 32.1 1.0 MG B:MG502 3.7 32.3 1.0 O1B B:GDP501 3.7 39.0 1.0 C B:SER140 3.8 31.6 1.0 O5' B:GDP501 3.9 37.9 1.0 O3A B:GDP501 3.9 38.4 1.0 C B:THR145 4.0 33.6 1.0 N B:THR145 4.0 32.5 1.0 O B:GLY143 4.0 32.3 1.0 O2A B:GDP501 4.1 38.5 1.0 C B:GLY144 4.2 32.3 1.0 CB B:SER140 4.2 32.2 1.0 PA B:GDP501 4.3 38.5 1.0 CA B:GLY144 4.3 31.8 1.0 N B:SER140 4.3 30.7 1.0 CA B:SER140 4.4 31.1 1.0 O B:GLY146 4.4 32.5 1.0 CA B:THR145 4.6 32.9 1.0 N B:LEU141 4.7 31.8 1.0 C B:GLY142 4.7 33.2 1.0 N B:GLY142 4.8 32.5 1.0 O B:THR145 4.8 34.4 1.0 O B:GLY144 4.8 32.4 1.0 OG B:SER147 4.8 35.5 1.0 C B:SER147 4.9 32.7 1.0 O3B B:GDP501 4.9 38.6 1.0 C B:LEU141 4.9 32.3 1.0 CA B:LEU141 5.0 31.7 1.0

Magnesium binding site 4 out of 4 in the Crystal Structure of the Apo Human Ttl in Complex with Tubulin- Stathmin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Crystal Structure of the Apo Human Ttl in Complex with Tubulin- Stathmin within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg501 b:33.2 occ:1.00 O C:HOH615 1.9 43.9 1.0 O2B C:GTP502 2.2 39.0 1.0 O C:HOH607 2.3 49.6 1.0 O2G C:GTP502 2.7 37.8 1.0 PB C:GTP502 3.5 38.8 1.0 OE2 C:GLU71 3.7 65.5 1.0 PG C:GTP502 3.8 37.8 1.0 CB C:GLN11 3.8 43.2 1.0 OD1 C:ASP69 3.9 52.1 1.0 O3B C:GTP502 3.9 38.2 1.0 OD2 C:ASP69 3.9 53.6 1.0 CG C:GLU71 4.0 56.8 1.0 O3A C:GTP502 4.1 39.4 1.0 O3G C:GTP502 4.1 37.4 1.0 N C:GLN11 4.2 41.6 1.0 OE1 C:GLN11 4.3 52.6 1.0 CG C:ASP69 4.3 51.8 1.0 CD C:GLU71 4.3 62.4 1.0 NZ D:LYS254 4.4 54.5 1.0 CA C:GLN11 4.6 41.8 1.0 O2A C:GTP502 4.6 40.0 1.0 OD2 C:ASP98 4.7 52.0 1.0 O1B C:GTP502 4.7 38.7 1.0 CB C:ASP98 4.8 44.9 1.0 PA C:GTP502 4.9 40.1 1.0 CG C:GLN11 4.9 46.3 1.0 CD C:GLN11 4.9 50.9 1.0

L.Vuillard, L.Miallau. Crystal Structure of the Apo Human Ttl in Complex with Tubulin-Stathmin To Be Published.