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Magnesium in PDB 8b6r: X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 Labeled with A Chloroalkane CYANINE3 Fluorophore Substrate

Enzymatic activity of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 Labeled with A Chloroalkane CYANINE3 Fluorophore Substrate

All present enzymatic activity of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 Labeled with A Chloroalkane CYANINE3 Fluorophore Substrate:
3.8.1.5;

Protein crystallography data

The structure of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 Labeled with A Chloroalkane CYANINE3 Fluorophore Substrate, PDB code: 8b6r was solved by M.Tarnawski, L.Hellweg, J.Hiblot, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 41.25 / 1.50
Space group P 42 21 2
Cell size a, b, c (Å), α, β, γ (°) 112.56, 112.56, 44.33, 90, 90, 90
R / Rfree (%) 16.2 / 19.2

Other elements in 8b6r:

The structure of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 Labeled with A Chloroalkane CYANINE3 Fluorophore Substrate also contains other interesting chemical elements:

Chlorine (Cl) 1 atom

Magnesium Binding Sites:

The binding sites of Magnesium atom in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 Labeled with A Chloroalkane CYANINE3 Fluorophore Substrate (pdb code 8b6r). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 Labeled with A Chloroalkane CYANINE3 Fluorophore Substrate, PDB code: 8b6r:

Magnesium binding site 1 out of 1 in 8b6r

Go back to Magnesium Binding Sites List in 8b6r
Magnesium binding site 1 out of 1 in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 Labeled with A Chloroalkane CYANINE3 Fluorophore Substrate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 Labeled with A Chloroalkane CYANINE3 Fluorophore Substrate within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg306

b:18.2
occ:1.00
O A:HOH547 2.0 19.4 1.0
O A:HOH426 2.1 21.1 1.0
OE2 A:GLU20 2.1 14.6 1.0
CD A:GLU20 3.0 16.3 1.0
OE1 A:GLU20 3.3 14.7 1.0
O A:HOH476 3.9 13.6 1.0
O A:GLY19 4.3 15.5 1.0
CG A:GLU20 4.4 12.0 1.0
CB A:PRO75 4.9 12.1 1.0
O A:HOH661 5.0 23.8 1.0

Reference:

L.Hellweg, A.Edenhofer, L.Barck, M.C.Huppertz, M.S.Frei, M.Tarnawski, A.Bergner, B.Koch, K.Johnsson, J.Hiblot. A General Method For the Development of Multicolor Biosensors with Large Dynamic Ranges. Nat.Chem.Biol. 2023.
ISSN: ESSN 1552-4469
PubMed: 37291200
DOI: 10.1038/S41589-023-01350-1
Page generated: Thu Oct 3 19:12:16 2024

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