Magnesium in PDB 8bms: Cryo-Em Structure of the Mutant Solitary Ecf Module 2EQ in MSP2N2 Lipid Nanodiscs in the Atpase Closed and Atp-Bound Conformation

The binding sites of Magnesium atom in the Cryo-Em Structure of the Mutant Solitary Ecf Module 2EQ in MSP2N2 Lipid Nanodiscs in the Atpase Closed and Atp-Bound Conformation (pdb code 8bms). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Cryo-Em Structure of the Mutant Solitary Ecf Module 2EQ in MSP2N2 Lipid Nanodiscs in the Atpase Closed and Atp-Bound Conformation, PDB code: 8bms:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Cryo-Em Structure of the Mutant Solitary Ecf Module 2EQ in MSP2N2 Lipid Nanodiscs in the Atpase Closed and Atp-Bound Conformation


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of the Mutant Solitary Ecf Module 2EQ in MSP2N2 Lipid Nanodiscs in the Atpase Closed and Atp-Bound Conformation within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:30.0 occ:1.00 O2B A:ATP301 2.0 36.3 1.0 OE1 A:GLN91 2.0 37.0 1.0 OG A:SER47 2.0 30.9 1.0 O3B A:ATP301 2.9 18.0 1.0 O1G A:ATP301 2.9 29.2 1.0 PB A:ATP301 3.0 18.5 1.0 CD A:GLN91 3.1 16.6 1.0 CB A:SER47 3.2 18.6 1.0 PG A:ATP301 3.5 17.7 1.0 NE2 A:GLN91 3.6 28.3 1.0 O1A A:ATP301 3.8 13.6 1.0 O3A A:ATP301 3.9 16.4 1.0 O1B A:ATP301 4.1 22.7 1.0 N A:SER47 4.2 3.6 1.0 OD1 A:ASP168 4.2 38.9 1.0 NE2 A:GLN169 4.3 30.8 1.0 O3G A:ATP301 4.3 26.1 1.0 CA A:SER47 4.3 8.6 1.0 CG A:GLN91 4.3 19.5 1.0 PA A:ATP301 4.4 11.4 1.0 OD2 A:ASP168 4.4 37.6 1.0 N B:GLY148 4.5 15.1 1.0 CB A:GLN91 4.5 23.6 1.0 CG A:ASP168 4.7 32.4 1.0 O2G A:ATP301 4.7 27.4 1.0 CB B:SER147 4.8 24.7 1.0 O2A A:ATP301 5.0 26.9 1.0

Magnesium binding site 2 out of 2 in the Cryo-Em Structure of the Mutant Solitary Ecf Module 2EQ in MSP2N2 Lipid Nanodiscs in the Atpase Closed and Atp-Bound Conformation


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Cryo-Em Structure of the Mutant Solitary Ecf Module 2EQ in MSP2N2 Lipid Nanodiscs in the Atpase Closed and Atp-Bound Conformation within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg302 b:34.1 occ:1.00 OG B:SER47 2.0 27.4 1.0 OE1 B:GLN92 2.0 39.7 1.0 O2B B:ATP301 2.1 31.9 1.0 O3G B:ATP301 2.2 26.7 1.0 CD B:GLN92 3.1 21.6 1.0 CB B:SER47 3.2 27.1 1.0 PB B:ATP301 3.3 11.2 1.0 PG B:ATP301 3.4 24.1 1.0 O3B B:ATP301 3.4 32.2 1.0 NE2 B:GLN92 3.7 26.8 1.0 O1A B:ATP301 3.7 28.2 1.0 O3A B:ATP301 4.0 12.2 1.0 N A:GLY146 4.2 21.7 1.0 OD2 B:ASP170 4.2 31.5 1.0 OD1 B:ASP170 4.3 41.7 1.0 N B:SER47 4.3 14.5 1.0 CA B:SER47 4.3 15.8 1.0 O2G B:ATP301 4.3 24.9 1.0 CG B:GLN92 4.4 24.2 1.0 PA B:ATP301 4.4 19.2 1.0 CB A:SER145 4.4 18.9 1.0 O1G B:ATP301 4.5 31.6 1.0 O1B B:ATP301 4.5 19.7 1.0 CB B:GLN92 4.5 26.6 1.0 CG B:ASP170 4.6 32.9 1.0 CA A:SER145 4.8 23.9 1.0 CA A:GLY146 5.0 18.8 1.0

C.Thangaratnarajah, M.Nijland, L.Borges-Araujo, A.Jeucken, J.Rheinberger, S.J.Marrink, P.C.T.Souza, C.Paulino, D.J.Slotboom. Expulsion Mechanism of the Substrate-Translocating Subunit in Ecf Transporters Nat Commun 2023.
ISSN: ESSN 2041-1723
DOI: 10.1038/S41467-023-40266-1