Magnesium in PDB 8bzd: Deac-Fc Stabilizer of 14-3-3 and Eralpha

The structure of Deac-Fc Stabilizer of 14-3-3 and Eralpha, PDB code: 8bzd was solved by E.J.Visser, C.Ottmann, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.45 / 1.50
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	82.1, 111.813, 62.529, 90, 90, 90
R / Rfree (%)	18.1 / 20.3

The binding sites of Magnesium atom in the Deac-Fc Stabilizer of 14-3-3 and Eralpha (pdb code 8bzd). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Deac-Fc Stabilizer of 14-3-3 and Eralpha, PDB code: 8bzd:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Deac-Fc Stabilizer of 14-3-3 and Eralpha


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Deac-Fc Stabilizer of 14-3-3 and Eralpha within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg301 b:32.6 occ:1.00 O A:HOH444 3.1 18.3 1.0 HZ1 A:LYS159 3.8 47.3 1.0 HZ2 A:LYS159 4.2 47.3 1.0 NZ A:LYS159 4.4 39.4 1.0 O A:HOH696 4.5 28.7 1.0 OE2 A:GLU189 4.6 20.4 1.0 O A:HOH633 4.7 29.0 1.0 HZ3 A:LYS159 4.7 47.3 1.0 O A:HOH592 4.7 36.5 1.0

Magnesium binding site 2 out of 3 in the Deac-Fc Stabilizer of 14-3-3 and Eralpha


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Deac-Fc Stabilizer of 14-3-3 and Eralpha within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:19.2 occ:0.65 O A:HOH491 2.4 27.9 1.0 OE1 A:GLU2 2.6 19.5 1.0 O A:HOH428 2.7 21.1 1.0 CD A:GLU2 3.6 22.0 1.0 OE2 A:GLU2 4.0 20.4 1.0 HA A:GLU2 4.0 19.6 1.0 H A:ARG3 4.2 18.0 1.0 O A:HOH584 4.5 21.7 1.0 O A:HOH688 4.7 23.1 1.0 CG A:GLU2 4.8 16.7 1.0 HB3 A:GLU2 4.9 20.6 1.0 CA A:GLU2 4.9 16.3 1.0 N A:ARG3 5.0 15.0 1.0

Magnesium binding site 3 out of 3 in the Deac-Fc Stabilizer of 14-3-3 and Eralpha


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Deac-Fc Stabilizer of 14-3-3 and Eralpha within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg303 b:24.8 occ:1.00 O A:HOH456 2.2 41.4 1.0 O A:HOH643 2.3 30.6 1.0 O A:HOH441 2.3 29.1 1.0 O A:HOH695 2.3 43.2 1.0 OE2 A:GLU89 2.4 23.2 1.0 O A:HOH610 2.4 33.2 1.0 CD A:GLU89 3.4 18.0 1.0 HG2 A:GLU86 3.6 24.1 1.0 HG2 A:GLU89 3.7 22.2 1.0 HH12 A:ARG85 3.9 25.7 1.0 HE22 A:GLN93 4.0 24.8 1.0 O A:HOH524 4.0 38.8 1.0 CG A:GLU89 4.1 18.5 1.0 HH11 A:ARG85 4.2 25.7 1.0 HB3 A:GLU89 4.3 19.1 1.0 OE1 A:GLU89 4.3 17.7 1.0 O A:HOH588 4.4 36.0 1.0 NH1 A:ARG85 4.4 21.4 1.0 HA A:GLU86 4.4 20.5 1.0 HG1 A:THR90 4.4 25.2 1.0 OE1 A:GLN93 4.5 19.6 1.0 CG A:GLU86 4.5 20.0 1.0 O A:HOH614 4.6 34.9 1.0 NE2 A:GLN93 4.7 20.6 1.0 CB A:GLU89 4.8 15.9 1.0 HB3 A:GLU86 4.8 21.0 1.0 OG1 A:THR90 4.8 21.0 1.0 HG3 A:GLU89 4.9 22.2 1.0 O A:HOH409 4.9 36.3 1.0 CD A:GLN93 5.0 20.1 1.0

E.J.Visser, M.Donaldson Collier, J.Siefert, M.Konstantinidou, S.Paul, P.Cossar, G.Miley, O.Meijer, M.R.Arkin, C.Ottmann, W.Zwart, L.Brunsveld. Stabilization of the Estrogen Receptor Alpha - 14-3-3 Interaction As A Potential Intervention Strategy For Endocrine Resistance in Breast Cancer To Be Published.